Seroprevalence of porcine and human influenza A virus antibodies in pigs between 1986 and 1988 in Hassia

1,268 sera collected from slaughtered pigs in Hassia (FRG) from 1986 to 1988 were tested for antibodies against porcine and human influenza A virus strains using the single radial haemolysis test (SRHT). Antibodies against the porcine strains (subtype H1N1) A/Swine/Arnsberg/1/81, A/Swine/Iowa/15/30 and A/New Jersey/7/76 were detected in 411 (32.4%), 318 (25.1%) and 304 (24.0%) of sera, respectively. Up to 1988 a slight increase (10%) in the seroprevalence to A/Swine/Arnsberg/1/81 was noticed, whereas the results obtained with the other strains showed little variation. Antibodies against the human H1N1 strain A/Singapore/6/86 were only found in sera collected 1987 and 1988 in rates of 1.6% and 3.0%. Serological indication of infections with the human H3N2 strains A/Victoria/1/75, A/Hong Kong/1/68 and A/Philippines/2/82 could be shown in 286 (22.6%), 178 (14.4%) and 135 (10.6%) of the serum samples. Within the three year period the rate of sera positive for antibodies against A/Philippines/2/82 increased from 6.5% to 23.0%, whereas no variation in the rates were found using the other H3N2 strains. Antibodies simultaneously against porcine (H1N1) and human (H3N2) virus strains were detected in 9.9% of all sera tested.     

Recovery of brodifacoum in vomitus following induction of emesis in dogs that had ingested rodenticide bait

AIM: To assess the benefit of inducing emesis in dogs that have ingested rodenticide bait containing brodifacoum (BDF), by determining the amount of BDF in bait recovered from the vomitus relative to the estimated amount consumed.

METHODS: Between 2014 and 2015 samples of vomitus from seven dogs that ingested rodenticide baits containing BDF were submitted by veterinarians in New Zealand. All seven dogs had been given apomorphine by the veterinarian and vomited within 1 hour of ingesting the bait. Some or all of the bait particles were retrieved from each sample and were analysed for concentrations of BDF using HPLC. Based on estimations of the mass of bait consumed, the concentration of BDF stated on the product label, and the estimated mass of bait in the vomitus of each dog, the amount of BDF in the vomited bait was calculated as a percentage of the amount ingested.

RESULTS: For five dogs an estimation of the mass of bait ingested was provided by the submitting veterinarian. For these dogs the estimated percentage of BDF in the bait retrieved from the vomitus was between 10–77%. All dogs were well after discharge but only one dog returned for further testing. This dog had a normal prothrombin time 3 days after ingestion.

CONCLUSIONS AND CLINICAL RELEVANCE: The induction of emesis within 1 hour of ingestion can be a useful tool in reducing the exposure of dogs to a toxic dose of BDF. The BDF was not fully absorbed within 1 hour of ingestion suggesting that the early induction of emesis can remove bait containing BDF before it can be fully absorbed.     

植保无人机低空低量施药雾滴沉积飘移分布立体测试方法

随着植保无人机在中国的广泛使用,植保无人机的沉积分布均匀性与雾滴飘移流失也引起各方面的重视。目前,针对植保无人机施药雾滴沉积飘移的测试方法较少,且着重于从沉积或飘移中某一方面分析植保无人机雾滴沉积飘移规律,未对作业中全方位的雾滴的沉积飘失规律进行系统测试。该文基于国际标准ISO22866和ISO24253建了1套针对低空低量植保无人机的立体测试方法,分别在地面布置沉积和飘移收集器,在空中架设立体沉积和空中飘移收集器,结合航拍影像所获取的植保无人机准确作业参数,对4个型号植保无人机分别搭载德国Lechler公司的IDK120-015和TR80-0067喷头进行了测试,系统分析了无人机周边的总沉积以验证方法准确性,计算了总地面沉降以表征可利用部分和空中耗散以评估环境风险。结果表明,各植保无人机地面沉积率在53.6%~76.6%,地面飘移率最高17.4%,空中飘移率可高达14.7%;该测试系统可收集62.4%~101.7%无人机喷洒出的雾滴。测试的4种植保无人机在搭载IDK喷头后均明显降低了雾滴飘移,但也同时降低地面沉积率;各植保无人机在搭载2种喷头时沉积规律不同,不同植保无人机设计需要选择不同喷头。该测试方法能够有效的收集并分析植保无人机在作业区域的雾滴立体分布状态,可为植保无人机综合评估提供新的参考依据。     

Comparative pathology and pathogenesis of spontaneous and experimentally induced fibropapillomas of green turtles (Chelonia mydas)

Tumor biopsy samples from 25 Floridian and 15 Hawaiian green turtles (Chelonia mydas) with spontaneous green turtle fibropapillomatosis (GTFP) and from 27 captive-reared green turtles with experimentally induced GTFP were examined microscopically to differentiate the histologic features that result from GTFP pathogenesis and those that result from incidental factors that may vary according to geographic region. Common histologic features for spontaneous and experimentally induced tumors included fibroblast proliferation in the superficial dermis, epidermal acanthosis and hyperkeratosis, epidermal basal cell degeneration with dermal-epidermal cleft formation, spinous layer degeneration with intraepidermal vesicle and pustule formation, and ulceration. Visceral tumors, found in eight of 10 (80%) free-ranging turtles with cutaneous disease that were examined after death, had extensive interstitial fibrous proliferation. The presence of spirorchid trematode eggs and associated foreign body granulomas, common secondary findings within spontaneous tumors, varied by geographic location, and these findings were not observed in experimentally induced tumors. Eosinophilic intranuclear inclusions and intranuclear herpesvirus-associated antigen immunoreactivity were found in 18 of 38 (47%) experimentally induced cutaneous tumors and nine of 119 (7.5%) spontaneous tumors from Floridian but not Hawaiian turtles. The possible involvement of GTFP-associated herpesvirus in the pathogenesis of epidermal degenerative changes and GTFP pathogenesis is discussed.     

The persistence of bovine enterovirus and pseudorabies virus in liquid cattle manure at different storage temperatures

The tenacity of viruses in liquid manure of cattle was examined in a total of five samples inoculated with ECBO-virus (strain LCR-4) representing viruses without envelope and Aujeszky virus (field isolate) representing enveloped viruses. The titers were examined at regular intervals over a period of 26 weeks. On the day of inoculation each sample had a titer of 10(5) ID50/ml. After 16 weeks complete inactivation was observed in the Aujeszky virus sample stored at 20 degrees C. The Aujeszky virus sample wich was kept at 4 degrees C at 26 weeks had a titer of 10(1,75) ID50/ml. In the samples inoculated with ECBO virus after 26 weeks of inoculation a titer of 10(3) ID50/ml was found in the manure stored at 20 degrees C. No influence on the virus titers in the liquid manure samples was observed either from pH or the number of bacteria (3,4 x 10(7)-1.16 x 10(8)/ml during the examination period.     

TELANG LIVER AND VITAMIN A TOXICITY

(1) About 20 per cent. of fresh telang livers were toxic to weanling rats receiving the calcium supplemented livers as their sole dietary. (2) The symptoms observed in rats were growth failure and multiple spontaneous fractures of the bones of the extremities. (3) The toxicity of certain telang livers was due to their abnormally high stores of vitamin A. When these livers, supplemented only with calcium carbonate, were fed to weanling rats, the daily intake of vitamin A was in excess of the toxic dosage of 15,000 I.U. per day. (4) Identical symptoms of toxicity were observed in weanling rats fed 20,000 I.U. of crystalline vitamin A per day in conjunction with a standard stock ration.     

Electron microscopic detection of herpesviruses in a mass death of Greek tortoises (Testudo hermanni) and four-toed turtles (Agrionemys horsfieldii)

In a population of about 130 tortoises, consisting mainly of Hermann's tortoises (Testudo hermanni) and Four-toed tortoises (Agrionemys horsfieldii), 50% of the reptiles died within 3 months in autumn 1987. Post mortem analysis of 5 necropsied turtles revealed thick, caseous coatings in the upper digestive tract, hepatomegaly and enteritis. By microbiological examination a lot of ubiquitous bacteria could be isolated. By electron microscopy of concentrated and negatively stained gut specimens herpes virus particles were found in two Hermann's tortoises and one Four-toed tortoise. Isolation experiments in embryonic turtle tissue cultures showed no evidence of a cytopathogenic agent. The results are discussed in respect of their importance for the high mortality observed in the population of mediterranean land turtles.