Curcumin nanoparticles: preparation, characterization, and antimicrobial study

Curcumin is a highly potent, nontoxic, bioactive agent found in turmeric and has been known for centuries as a household remedy to many ailments. The only disadvantage that it suffers is of low aqueous solubility and poor bioavailability. The aim of the present study was to develop a method for the preparation of nanoparticles of curcumin with a view to improve its aqueous-phase solubility and examine the effect on its antimicrobial properties. Nanoparticles of curcumin (nanocurcumin) were prepared by a process based on a wet-milling technique and were found to have a narrow particle size distribution in the range of 2-40 nm. Unlike curcumin, nanocurcumin was found to be freely dispersible in water in the absence of any surfactants. The chemical structure of nanocurcumin was the same as that of curcumin, and there was no modification during nanoparticle preparation. A minimum inhibitory concentration of nanocurcumin was determined for a variety of bacterial and fungal strains and was compared to that of curcumin. It was found that the aqueous dispersion of nanocurcumin was much more effective than curcumin against Staphylococcus aureus , Bacillus subtilis , Escherichia coli , Pseudomonas aeruginosa , Penicillium notatum , and Aspergillus niger . The results demonstrated that the water solubility and antimicrobial activity of curcumin markedly improved by particle size reduction up to the nano range. For the selected microorganisms, the activity of nanocurcumin was more pronounced against Gram-positive bacteria than Gram-negative bacteria. Furthermore, its antibacterial activity was much better than antifungal activity. The mechanism of antibacterial action of curcumin nanoparticles was investigated by transmission electron micrograph (TEM) analysis, which revealed that these particles entered inside the bacterial cell by completely breaking the cell wall, leading to cell death.     

Growth,symbiosis, productivity,and profitability of soybean at varying planting methods and nitrogen levels

A field experiment was conducted to study the effect of different planting methods (Happy Seeder sowing, Straw Chopper + Zero Tillage sowing, and conventional sowing) and nitrogen (N) levels (0%, 75%, 100%, and 125% of recommended N) on the emergence, growth, symbiotic parameters, productivity, and profitability of soybean sown after wheat harvest. Growth and symbiotic parameters were significantly better under Happy Seeder sowing and Straw Chopper + Zero Tillage sowing than conventional sowing. The seed yield, nutrient uptake, and economic returns were significantly higher under Happy Seeder sowing than other methods. The growth parameters, symbiotic parameters, biological, straw, and seed yields were increased significantly up to 100% recommended N level. The nutrient uptake increased significantly up to 100% recommended N level. Therefore, sowing with Happy Seeder after combine harvested wheat along with 100% recommended N would best optimize soybean yield and profitability.     

Subclinical endometritis in estrual buffaloes: diagnosis,prevalence and impact on reproductive performance

Tropical Animal Health and Production - The study evaluated the reliability of polymorphonuclear (PMN) cell count in endometrial cytology by cytobrush technique as a diagnostic for subclinical...     

Rice yield variability in Punjab: an overview of five decades

Rice is a major kharif cereal crop cultivated in Punjab. Over the past five decades the rice yield has increased from as low as 1800 kg/ha during 1970–4815 kg/ha during 2017, i.e., an overall increase of 167.5% or increase @ 3.5% per year. One of the major reasons for this increase has been the introduction of improved and high-yielding varieties (PR 106, PR 111, PR 116, PR 118, PR 121, PR 122, PR 126, PR 127, etc.). Other important factors are the improved crop production and protection practices coupled with mechanization of farm operations. However, weather factors have always been a major bottleneck shattering the steady plateau of high rice yield (3443 kg/ha during 1979, 3231 kg/ha during 1995, 3273 kg/ha during 1998, 4257 kg/ha during 2011 and recent low yield of 4387 kg/ha during 2018). The actual weather data of the recent two decades at Ludhiana (2000–2018) were correlated with the historical rice yield. The results revealed a combination of high temperature coupled with clear sunny (total seasonal sunshine?~?900–1000 h) and dry (low relative humidity) days is conducive for proper growth, development and yield of rice in Punjab. The rainfall and rainy days were found to be negatively correlated with yield since heavy rainfall events and more number of rainy days create a humid weather which is conducive for insect-pest and disease incidence in rice and also reduces sunshine duration. Hence, a combination of ample sunshine hours and well-distributed rainfall during the vegetative stage of rice leads to high productivity of cultivars grown under optimized crop management techniques.

     

Effect of Tillage,Crop Residues of Preceding Wheat Crop and Nitrogen Levels on Biological and Chemical Properties of Soil in the Soybean–Wheat Cropping System

A 2-year field experiment was conducted to study the influence of three planting methods (Happy Seeder, Straw Chopper + Zero Tillage, and Conventional tillage) and four nitrogen rates (control, 75, 100, and 125% of recommended nitrogen) on the chemical and biological properties of soil. The results revealed that after soybean harvest, dehydrogenase activity and population of Bradyrhizobium sp. (LSBR 3) and plant growth promoting rhizobacteria (PGPR) were increased significantly in Happy Seeder sowing and Straw Chopper + Zero Tillage sowing than conventional sowing and population of Bradyrhizobium sp. significantly increased up to the 100% nitrogen level. There was no significant effect of different planting methods on soil chemical properties. Organic carbon and available nitrogen were increased significantly with an increase in the nitrogen level up to 100% nitrogen (N).     

Ecofriendly management of stem rot of berseem caused by Sclerotinia trifoliorum

Stem rot, incited by Sclerotinia trifoliorum Eriksson is one of the most devastating soil borne fungal pathogens of berseem (Trifolium alexandrinum L.) inflicting significant fodder and seed yield losses. Eleven plant extracts, six organic inputs, one elicitor and five organic and inorganic salts were assayed for their effectiveness against S. trifoliorum under in vitro conditions. Extracts of Aegle marmelos and Cymbopogan citrates showed complete mycelial inhibition of S. trifoliorum at 5% concentration. Among organic inputs, panchgavya and fermented cow urine recorded 100% inhibition to mycelial growth of stem rot pathogen at 10% concentration. Elicitor (chitosan) and salts namely copper sulphate and potassium carbonate provided complete mycelial inhibition of test pathogen under in vitro conditions at 0.1, 2.0 and 5.0% concentrations, respectively. The plant extracts, organic inputs and salts that exhibited highest mycelial inhibition to S. trifoliorum under in vitro conditions were evaluated for their antifungal efficacy under greenhouse and field experiments during rabi 2017–18 and 2018–19. The extracts of chitosan (78.58; 77.15%), panchgavya (75.99; 67.14%), copper sulphate 71.38; 61.18%) and A. marmelos (71.75; 56.24%) have recorded highest percent disease control as compared to untreated control in greenhouse and field trials in rabi 2017–18 and 2018–19, respectively. Significant increase in green fodder yield of berseem from 9.87 to 33.38% was also recorded after treatment with plant extracts, organic inputs and some salts. The current study showed that plant extracts, organic inputs and salts have potential for the management of stem rot of berseem.

     

Crop establishment method and irrigation schedule effect on water productivity,quality, economics and energetics of aerobic direct-seeded rice (<Emphasis Type="Italic">Oryza sativa</Emphasis> L.)

Rice is cultivated through transplanting of seedling in submerged field which is a cumbersome, labour intensive and water-guzzling practice. A field experiment was conducted to study the effect of crop establishment methods and irrigation schedules on water productivity, economics and energetics of aerobic direct-seeded rice at Punjab Agricultural University, Ludhiana, India, during Kharif 2012–2013. The experiment was laid out in split plot design, keeping combinations of two tillage system (no-tillage and conventional tillage) and two methods of sowing (uni-directional and bi-directional) in main plots and four irrigation schedule [(30, 45, 60 and 75 mm CPE (cumulative pan evaporation)] in sub plots. Aerobic direct-seeded rice sown after conventional tillage gave significantly higher grain yield than no-till with 15.4 % higher water expense efficiency. The energy gain and net monetary returns were 13.2 and 21.2 % higher in conventional sown crop than no-till, respectively. Bi-directional sowing resulted in 26.5 % higher grain yield than uni-directional with no effect on quality traits of grains. The net energy gain and net monetary returns were 26.5 thousands MJ/ha and 125.3 $/ha higher from bi-directional sown crop than uni-directional sown crop. Crop irrigated at 30 mm CPE schedule resulted in significantly higher grain yield than that irrigated at 45, 60 and 75 mm CPE. The energy gain, energy use efficiency and net returns were also maximum at 30 CPE schedule than at 45, 60 or 75 CPE. However, brown, milled and head rice recoveries were statistically at par between irrigation scheduling at 30 and 45 mm CPE but significantly better than 60 and 75 mm CPE. Bi-directional sowing with conventional tillage and irrigation at 30 CPE is an energy efficient and economical viable technique for direct-seeded rice.