岭西电网经济分析

本文对岭西电网的经济运行调整进行了分析、总结。并应用《岭西电网经济运行计算》系统的计算结果，提出了岭西电网的经济运行调整办法。     

黄麻（新、引进）品种在闽南地区的产量（鉴定）试验

通过对中国农科院麻类所新选育的3个黄麻圆果种和12个国外引进的黄麻优异品种进行2002-2005年品种比较试验，结果表明：C90-2、C90-6、C89-6比对照种粤圆五号增产14．97％-19．34％，均达极显著水平。O-3、O-1、O-3（红茎）比对照种宽叶长果增产32．02％-34．21％，均达极显著水平。O-4（Ⅱ）比对照种增产12．28％达显著水平。C-1、C-5比对照种粤圆五号增产11．90％和7．82％。且主要经济性状优良，抗病力强。黄麻品种C90-2、C90-6、C89-6、O-3、O-1、O-3（红茎）、0-4（Ⅱ）、C-1和C-5适合于福建闽南麻区推广种植。     

Influences of chronic hypoxia on the gene expression of Kv1.3,Kv2.1 and Kv3.1 induced by acute hypoxia in PASMC of rats

AIM and METHODS: Total RNA was extracted from 6th rat subcultured pulmonary artery smooth muscle cells(PASMC) exposed to continual chronic hypoxia or normoxia and the effects of chronic hypoxia on the changes of Kv1.3,Kv2.1,Kv3.1 mRNA in cultured PASMC induced by acute hypoxia were studied by semiquantitative RT-PCR in vitro. RESULTS:①Kv1.3,Kv2.1,Kv3.1 genes were found to be expressed in PASMC of rats exposed either to hypoxia or normxia.②The expression of Kv2.1 and Kv3.1 in 6th subcultured of PASMC in normaxia group could be upregulated by exposure to acute hypoxia,the levels of Kv2.1 and Kv3.1 mRNA were significantly increased from 0.646±0.092, 0.782±0.104 to 1.059±0.134, 0.985±0.116,respectively (P<0.01,n=5). ③PASMC cultured continuously in chronic hypoxia for 6 subcultures and then exposed to normoxia for 12 h,thereafter the expression of Kv2.1 and Kv3.1 were downregulated by acute hypoxia for 6 hours.The level of Kv2.1 mRNA was significantly decreased from 1.008±0.117 to 0.649±0.097 (P<0.01,n=5). CONCLUSION:Kv2.1,Kv3.1 genes might be oxygen sensitive genes.Chronic hypoxia might change the response of these Kv genes of PASMC to acute hypoxia and down-regulate its expression,which might probably decrease the role of Kv in HPV.     

Effects of blocking the expression of PKARⅠβ gene with antisense nucleotide on Shuang long-Jie gu Pill (SLJGP) containing serum osteoblast proliferation

AIM: To further investigate the role of PKARⅠβ in the growth-promoting effects of shuang long Jiegu pill (SLJGP), a Chinese medicine, on cultured osteoblasts. METHODS: pcDNA- antiPKARⅠβ, a recombinant expressing the antisense sequence of PKARⅠβ, was constructed and transformed HFOB1.19 by lipofectin. MTT was undertaken to assess the cell growth with the treatment of high dosage of SLJGP containing serum. RESULTS: Antisense gene blocked the growth-promoting effects of SLJGP containing serum on HFOB1.19. CONCLUSION: The function of SLJGP is closely related to cAMP-dependent protein kinase A.     

致麻鸭产蛋下降的副粘病毒的分离和鉴定

从浙江省某麻鸭养殖基地产蛋锐减的病鸭生殖器官分离到1株致产蛋下降、不致鸭死亡的病毒株(YH99V)。该病毒易感蛋鸭，经SPF鸡胚传至第9代时致病性突然增强，第11代出现对鸡红细胞的血凝特性，通常在42～80h致死SPF鸡胚，EF15EID50为10^1.8。经磷钨酸负染电镜观察，YH99V粒子呈现圆形、杆状形、葫芦状形等多形态，直径70～400nm不等，病毒外表有囊膜，囊膜外层有排列整齐的纤突。病毒粒子和包涵体位于细胞浆内。病毒抵抗力由弱到强依次为：0．2%甲醛、24h-56℃、45min→紫外线、1h→乙醚≈氯仿≈37℃、16h—pH9→pH5→1％Try。对人眼结膜易感，鸭眼结膜不易感。接种传代细胞BHK-21、IBRS-2均能引起细胞病变。YH99V株与同样引起鸭产蛋下降的AIV、鸭源EDSV无抗原相关性，而能被禽副粘病毒Ⅰ型阳性血清中和。根据试验结果，初步将YH99V判定为鸭副粘病毒。     

宁夏地区烟粉虱对5种常用杀虫剂的抗药性

采用浸叶法分别测定了宁夏8个地区烟粉虱成虫田间种群对4类5种杀虫剂的抗性.结果 表明,供试烟粉虱种群对高效氯氰菊酯已产生了中等至极高水平抗性,抗性倍数为33.31～227.98倍;对烯啶虫胺产生了中等至高水平抗性,抗性倍数为12.14～69.33倍;对毒死蜱和吡虫啉产生了低至中等水平抗性,抗性倍数分别为7.63～38....     

福建省木材流通领域监督管理的探讨

围绕我省近年来木材运输、经营加工管理和监督检查工作展开探讨,分析了木材流通中的特点和变化,同时针对木材流通中存在的问题,提出了一些建设性的意见。     

基于微课的《养猪学》课程混合式教学探究

针对动物科学专业《养猪学》课程理论知识落后于现代化的生产实践,以及学生对课程的学习缺乏兴趣的问题,将在线的教学模式-微课与面授的教学模式相融合,形成特色的《养猪学》混合教学模式,从而丰富课程内容、提升学生学习积极性,最终提高教学质量。     

都市餐饮企业的净菜需求影响因素分析——以北京市为例

净菜的广泛使用能提高餐饮企业的出餐率,减少其后厨面积、劳动用工和厨余垃圾.基于北京地区708份餐饮企业净菜需求的问卷调查样本,运用垄断竞争市场理论和二元选择模型分析了影响餐饮企业净菜使用行为的具体因素.研究发现,对净菜了解程度不够和净菜价格偏高是制约餐饮企业使用净菜的主要因素,具有不同个体特征和环境特征的餐饮企业选择净...