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1.
Fortes Maira Salomão Lopes-Mori Fabiana Maria Ruiz Caldart Eloiza Teles Constantino Caroline Evers Fernanda Pagliari Sthefany de Almeida Jonatas Campos Barros Luiz Daniel Freire Roberta Lemos Garcia João Luis Headley Selwyn Arlington Navarro Italmar Teodorico 《Tropical animal health and production》2018,50(2):413-419
Tropical Animal Health and Production - This study investigated the prevalence of caprine toxoplasmosis in goat herds from Southern Brazil by the indirect immunofluorescence assay (IFA), and... 相似文献
2.
C.?Silvar J.?M.?Duncan D.?E.?L.?Cooke N.?A.?Williams J.?Díaz F.?MerinoEmail author 《European journal of plant pathology / European Foundation for Plant Pathology》2005,112(1):43-52
A PCR-based method was developed for the identification and detection of Phytophthora capsici in pepper plants. Three PCR primers (CAPFW, CAPRV1 and CAPRV2) specific for P. capsiciwere designed based on the sequence of its internal transcribed spacer regions. CAPFW/CAPRV1 amplify a 452 bp product from P. capsici DNA whereas CAPFW/CAPRV2 a 595 bp fragment; neither set amplifies DNA from pepper or several fungi pathogenic to pepper. In conventional (single-round) PCR, the limit of detection was 5 pg DNA for both primer sets, whereas in nested PCR the detection limit for both was of 0.5 fg. However, when the dilution series of target DNA were spiked with plant DNA, amplification declined two-fold in both conventional and nested PCR. The CAPFW/CAPRV2 set in conventional PCR was used to detect P. capsici DNA in inoculated plants. Detection occurred as soon as 8h post-inoculation in stem samples from infected but still symptomless plants. The method was also tested to detect fungal DNA in infected soils. 相似文献
3.
Balaro Mario Felipe A. de Mello Samuel Guaraná Valverde da Silva Santos Alex Cavalcanti Luiza Mattos Almosny Nádia Regina Pereira Fonseca Jeferson F. Brandão Felipe Z. 《Tropical animal health and production》2019,51(2):345-353
Tropical Animal Health and Production - This study aimed to verify the reproductive seasonality in Saanen goats from distinct parity orders (nulliparous, primiparous, and pluriparous) throughout an... 相似文献
4.
Rosangela Simeão Adriane Silva Cacilda Valle Marcos Deon Resende Sérgio Medeiros 《Plant Breeding》2016,135(2):246-253
Half‐sib progenies of congo signal grass (Brachiaria ruziziensis Germain & Evrard, syn.: B. eminii, Urochloa ruziziensis) were analysed using a mixed model methodology. The objective was to estimate genetic and phenotypic parameters, predict individual genetic values, estimate correlations between characters associated with biomass production and forage nutritive value and use this information to determine the economic weights to compare three multiplicative selection indices. Individual narrow‐sense heritabilities corrected for inbreeding varied from 0.14 to 0.91 for characters associated with biomass production and from 0.04 a 0.24 for nutritional value characters. The correlations among characters associated with annual biomass production were of high magnitude. In contrast, biomass production characters were negatively correlated with crude protein, acid detergent fibre and lignin. Total dry matter yield, crude protein and regrowth ability were weighted separately in evaluations during wet and dry season or annually in selection indices. Index that considered characters annually resulted in better distribution of forage production along the year than indices that separated wet and dry season production. 相似文献
5.
Luciana Linhares de Azevedo Bittencourt Kelly Alencar Silva Valéria Pereira de Sousa Gizele Cardoso Fontes-Sant’Ana Maria Helena Rocha-Leão 《Plant foods for human nutrition (Dordrecht, Netherlands)》2018,73(4):278-286
In the processing of fruits such as blueberry (Vaccinium sp), that has high levels of phenolic acid, the food industry produces tons of organic waste that causes harm to the environment. Encapsulation is a technique used to take advantage of these wastes. Several methods are used to encapsulate substances, among them ionotropic gelation proves to be a simple, precise, efficient and economical method for obtaining particles with encapsulated bioactives. In this manner, the aim of this study was to test sodium alginate as wall material to encapsulate blueberry residue by ionotropic gelation. The microbeads were characterized by scanning electron microscopy (SEM), x-ray diffraction (XRD), total phenolic compounds, antioxidant capacity and in vitro dissolution. The results showed that the microbeads had surface invagination; retention of 67.01% of the phenolic compounds after encapsulation and 68.2%, phenolic release 120 min after in vitro dissolution. The results suggest that the tested matrix was suitable for encapsulation. The produced microbeads are promising for applications in food products, once the phenolic compounds present in the blueberry residues were maintained after encapsulation. 相似文献
6.
Tatiana da Rosa Guimar?es Carlos Guillermo Quiroz Caroline Rigotto Simone Quintana de Oliveira Maria Tereza Rojo de Almeida éverson Miguel Bianco Maria Izabel Goulart Moritz Jo?o Luís Carraro Jorge Alejandro Palermo Gabriela Cabrera Eloir Paulo Schenkel Flávio Henrique Reginatto Cláudia Maria Oliveira Sim?es 《Marine drugs》2013,11(11):4176-4192
The n-butanol fraction (BF) obtained from the crude extract of the marine sponge Petromica citrina, the halistanol-enriched fraction (TSH fraction), and the isolated compounds halistanol sulfate (1) and halistanol sulfate C (2), were evaluated for their inhibitory effects on the replication of the Herpes Simplex Virus type 1 (HSV-1, KOS strain) by the viral plaque number reduction assay. The TSH fraction was the most effective against HSV-1 replication (SI = 15.33), whereas compounds 1 (SI = 2.46) and 2 (SI = 1.95) were less active. The most active fraction and these compounds were also assayed to determine the viral multiplication step(s) upon which they act as well as their potential synergistic effects. The anti-HSV-1 activity detected was mediated by the inhibition of virus attachment and by the penetration into Vero cells, the virucidal effect on virus particles, and by the impairment in levels of ICP27 and gD proteins of HSV-1. In summary, these results suggest that the anti-HSV-1 activity of TSH fraction detected is possibly related to the synergic effects of compounds 1 and 2. 相似文献
7.
B Costas P C N P Rêgo I Simões J F Marques M Castro‐Cunha A Afonso 《Journal of fish diseases》2013,36(6):543-553
The present study aimed to investigate leucocyte responses to inflammation as well as some innate immune parameters of Senegalese sole, Solea senegalensis, following challenge with two strains of Photobacterium damselae subsp. piscicida belonging to the European and Japanese clones described for this bacterium. Pathogenicity assays were performed to assess the virulence of each Photobacterium damselae subsp. piscicida strain for sole. Subsequently, fish were intraperitoneally injected with phosphate‐buffered saline (control) or two concentrations (2 × 102 and 2 × 106 CFU mL?1) of each bacterial strain and sampled after 6 and 24 h. Results showed that the European isolate induces a higher degree of response than the Japanese strain. While blood neutrophilia and monocytosis correlated well with the increase in neutrophil and macrophage numbers in the peritoneal cavity, fish infected with the European isolate presented higher peritoneal cell numbers than fish challenged with the Japanese strain. In addition, alternative complement pathway activity and respiratory burst of head kidney leucocytes increased significantly in fish infected with the European isolate. The enhanced innate immune response displayed by Senegalese sole challenged with the European isolate is probably due to the higher degree of virulence presented by this Photobacterium damselae subsp. piscicida strain. 相似文献
8.
Castro Liliane Araújo Andrade-Porto Sanny Maria Oliveira Rebeca Guimarães Batista Yasmin Luzeiro Silva Felipe Moura Araújo Oliveira Christiane Patrícia Feitosa Affonso Elizabeth Gusmão Cruz Claudinei Tavares-Dias Marcos 《Aquaculture International》2021,29(6):2477-2488
Aquaculture International - This study investigated the efficacy of trichlorfon against Neoechinorhynchus buttnerae, as well as its palatability, residue analysis, and the blood parameters of... 相似文献
9.
Portugal's forests in the 12th century were dominated by the Fagaceae represented by Quercus and Castanea, and several pine species.From the 12th century onwards, forests underwent changes in their management starting with protection and ultimately leading to intense exploitation. The massive naval construction during the maritime expansion(mainly in the 15th–16th centuries) involved felling of approximately 5 million trees mainly Quercus suber, Pinus pinea and other Quercus species. Cumulative fuel-wood consumption of 959 Mm3 during 1300–1854 was attributed to demographic expansion while the deforestation rate during 1636–1854 accounted for a minimum of 72.6% and a maximum of 96% of total forest cover. The volume of timber used in railway sleepers from 1856 onwards might have reached 0.5 Mm3. The last quarter of the 20th century increased the forest cover of Portugal through the World Bank program of Eucalyptus globulus reforestation. 相似文献
10.
L.?JiménezEmail author I.?Pérez J.?C.?García F.?López J.?Ariza 《Wood Science and Technology》2004,38(2):127-137
This paper reports on the influence of independent variables in the pulping of wheat straw (viz. cooking temperature and time, and ethanol concentration), and of the number of PFI beating revolutions to which the pulp is subjected, on the yield and Shopper-Riegler index of the pulp, and on the breaking length, stretch, burst index and tear index of the resulting paper sheets. By using a central composite factor design and the BMDP software suite, equations that relate each dependent variable to the different independent variables were obtained that reproduce the experimental results for the dependent variables with errors less than 25% at temperatures, times, ethanol concentrations and numbers of PFI beating revolutions in the ranges 140–180°C, 60–120 min, 40–80% and 0–1750, respectively. Ensuring the optimal Shopper-Riegler index for the pulp (73.18°SR), and also the optimal breaking length (6276 m), stretch (2.57%) and burst index (3.72 kN/g) for the papers sheets, entails using a high temperature (180°C), a large number of PFI beating revolutions (1750) and medium-to-high ethanol concentration and time values; these conditions, however, decrease the tear index for the paper sheets below the optimum level (4.60 mNm2/g). A high temperature and a large number of PFI beating revolutions, in conjunction with a low ethanol concentration (60%) and a short time (60 min), can be used to save solvent and increase the production; the values thus obtained for the dependent variables differ by less than 30% from their optimum levels in the worst case. 相似文献