Type-I hypersensitivity as a component of eosinophilic myositis (muscular sarcocystosis) in cattle

Eight bovine hearts with lesions of eosinophilic myositis (EM) and 2 bovine hearts without EM lesions were collected at slaughter. Blood samples from these 10 hearts, and the heart of a newborn calf also were collected. Histologically, Sarcocystis cruzi was identified in the 8 hearts with EM lesions and the 2 hearts without EM lesions, but not in the heart of the newborn calf. Serum was harvested from the 10 blood samples and was used in homologous, modified, passive cutaneous anaphylaxis tests. Antigen was prepared from S cruzi bradyzoites isolated from the 2 hearts without EM lesions. Serum samples from the 8 cattle with EM lesions reacted positively to S cruzi antigen. When heat-inactivated IgE in serum (56 C for 4 hours) was used, all passive cutaneous anaphylaxis responses were considered negative. Using ELISA, serum IgE concentrations from the 10 cattle with and without EM lesions were 2.2 to 9 U/ml. As determined by radial immunodiffusion, IgM concentrations were 80 to 215 mg/dl. Immunoglobulin G concentrations were 420 to 2,050 mg/dl, but most were less than or equal to 1,700 mg/dl. Immunoglobulin A concentrations were 0 to 62 mg/dl; 1 steer with EM lesions had 0 mg/dl. Double-gel immunodiffusion confirmed the presence of Sarcocystis-specific precipitating antibodies. Sera from the 10 cattle with and without EM lesions formed at least 1 precipitin band.     

Studies on the potential use of Pasteuria penetrans as a biocontrol agent of root-knot nematodes (Meloidogyne spp.)

Some aspects of the interaction of the bacterial parasite Pasteuria penetrans and the root-knot nematode ( Meloidogyne spp.) were investigated in laboratory and pot experiments. The variable spore attachment on juveniles exposed to water suspensions of the bacterium is probably attributed to differential susceptibility of biotypes within a heterogeneous Meloidogyne population. The relationship between spore concentration and attachment level is not linear over a range of spore dosages, indicating that even at very high spore concentrations the number of spores capable of attachment may not be present in excess and it is difficult to ensure sufficient numbers of spores to ensure infection will attach to all nematodes. Attempts to apply the bacterium in conditions such as might occur in seedbeds did not suppress nematode multiplication after transplanting in nematode-infested soil, indicating that the only effective application method is a thorough spore distribution in the planting sites. Two major constraints were revealed: high levels of spore attachment to juveniles does not always guarantee a significant reduction of egg laying and this is greatly influenced by the Meloidogyne biotype. Furthermore, the cumulative effect of the parasite in reducing Meloidogyne populations over several crop cycles was less than expected as the bacterium reduced intra-specific competition for the food supply and the less damaged root enabled many nematodes to survive.     

Feeding fodder beet (Beta vulgaris L.) with either barley straw or pasture silage to non-lactating dairy cows

AIMS: To determine the suitability of diets containing either approximately 85% fodder beet (Beta vulgaris L.) with barley straw or 65% fodder beet with pasture silage when fed to non-lactating dairy cows, by measuring intakes, digestibility, rumen function including microbial growth, and N excretion.

METHODS: Holstein-Friesian cows fitted with permanent rumen fistulae were fed either 65% fodder beet with pasture silage (Silage; n=8) or 85% fodder beet with barley (Hordeum vulgare L.) straw (Straw; n=8) in an indoor facility over a 9-day period, for measurement of intakes, digestibility, rumen function and urine production. The cows were adapted to the diets over 2 weeks before the indoor measurements. Feed was available for about 6 hours/day, as practiced commercially for wintering non-lactating cows.

RESULTS: Five cows fed the Straw diet had to be removed from the trial because of acute acidosis; four on Day 1 of the measurement period and one on Day 7. One cow allocated to the Silage diet refused to eat fodder beet bulbs and was also removed from the trial. Two cows fed the Silage diet were also treated for acidosis. DM intakes were lower with the Straw than Silage diets (6.4 (SE 0.4) vs. 8.3 (SE 0.5) kg/day) and organic matter (OM) digestibility was lower with the Straw than Silage diets (77 (SE 1) vs. 83 (SE 1) g/100g). The N content of the two diets was 1.14 and 1.75?g/100?g DM and there was a net loss of N by cows fed the Straw diet (?22.7 (SE 7) g/day). Rumen microbial N production was much lower in cows fed the Straw than the Silage diet (6.6 (SE 1.3) vs. 15.8 (SE 0.7) g microbial N/kg digestible OM intake). Concentrations of ammonia in rumen liquid collected on Days 5–6 were below detection limits (<0.1?mmol/L) in 36/48 (75%) samples collected from cows fed the Straw diet and in 27/48 (56%) cows fed the Silage diet. Mean urinary N excretion was lower in cows fed the Straw than the Silage diet (52.0 (SE 5.8) vs. 87.7 (SE 5.9) g/day).

CONCLUSION AND CLINICAL RELEVENCE: An over-wintering diet for dry cows comprising about 65% fodder beet with 35% pasture silage provided adequate nutrition, although there was some risk of acidosis. In contrast, the diet containing about 85% fodder beet with barley straw resulted in lower DM intakes, poor rumen function, negative N balance so that both nutrition and welfare were compromised.     

豪猪源福氏志贺菌的分离鉴定及喹诺酮类耐药基因检测

为调查四川省某规模化豪猪养殖场中豪猪大面积腹泻死亡的原因,本研究采集病死豪猪的肠道内容物和肺脏等病料样品进行细菌的分离纯化,通过培养特性观察、革兰染色镜检、生化鉴定、16S rRNA基因的克隆测序进行鉴定,并对分离菌株进行致病性、耐药性分析,对其喹诺酮类耐药基因进行PCR检测并测序分析。结果显示,分离得到1株革兰阴性菌,该分离株符合福氏志贺菌的培养特性和生化特性,并且其16S rRNA基因序列与福氏志贺菌该基因序列的同源性为99%;对小鼠具有较强致病性;药敏试验结果显示分离株具有多重耐药性,仅对丁胺卡那敏感,对喹诺酮类、四环素类、头孢类、氨基糖苷类、青霉素类和氯霉素类药物均表现为耐药;分离株的DNA促旋酶Ⅱ和拓扑异构酶Ⅳ的喹诺酮耐药决定区均有突变,且携带有质粒介导的喹诺酮耐药基因qnr A和qnr S。本研究是国内首次关于豪猪源福氏志贺菌病的报道,为豪猪养殖中该细菌性疾病的诊断防治等相关研究提供参考。     

Solidworks在机床夹具设计中的应用

有别与传统的CAD二维绘图,利用Solidworks绘制三维机床夹具可以非常直观、形象的显示设计结构与结果.本文介绍如何利用Solidworks软件绘制机床夹具三维图形.     

In vitro susceptibility of Brachyspira hyodysenteriae to organic acids and essential oil components

The antibacterial potential of organic acids and essential oil components against Brachyspirahyodysenteriae, the causative pathogen of swine dysentery, was evaluated. Minimum inhibitoryconcentrations (MIC) of 15 compounds were determined at pH 7.2 and pH 6.0, using a broth microdilution assay.In addition, possible synergism was determined. MIC values for the three tested strains were similar. Fororganic acids, MIC values at pH 6.0 were lower than at pH 7.2. B. hyodysenteriae was mostsensitive to cinnamaldehyde and lauric acid, with MIC values <1.5 mM. Most antibacterial effects of binarycombinations were additive, however, for thymol and carvacrol, synergism could be observed. Invitro results demonstrate the antibacterial action of certain essential oil components and organicacids against B. hyodysenteriae.     

Acute oral toxicity of zinc phosphide: an assessment for wild house mice (Mus musculus)

Irregular plagues of house mice, Mus musculus, incur major economic impacts on agricultural production in Australia. The efficacy of zinc phosphide (ZnP), the only registered broadacre control agent for mice, is reported as increasingly variable. Have mice become less sensitive over time or are they taking a sub-lethal dose and developing aversion? In this laboratory study, the sensitivity of mice (wild caught; outbred laboratory strain) was assessed using oral gavage of a range of ZnP concentrations. The estimated LD₅₀ values (72–79 mg ZnP/kg body weight) were similar for each mouse group but are significantly higher than previously reported. The willingness of mice to consume ZnP-coated grains was determined. ZnP-coated grains (50 g ZnP/kg grain) presented in the absence of alternative food were consumed and 94% of wild mice died. Mice provided with alternative food and ZnP-coated wheat grains (either 25 or 50 g ZnP/kg grain) consumed toxic and non-toxic grains, and mortality was lower (33–55%). If a sublethal amount of ZnP-coated grain was consumed, aversion occurred, mostly when alternative food was present. The sensitivity of wild house mice to ZnP in Australia is significantly lower than previously assumed. Under laboratory conditions, ZnP-coated grains coated with a new higher dose (50 g ZnP/kg grain) were readily consumed. Consumption of toxic grain occurred when alternative food was available but was decreased. Our unambiguous findings for house mice indicate a re-assessment of the ZnP loading for baits used for control of many rodents around the world may be warranted.     

我国野生披碱草属牧草居群的穗部形态多样性研究

对采集自我国12个不同分布区的105个野生披碱草属(Elymus)牧草居群的14项穗部形态指标进行研究,结果表明,我国披碱草属牧草种质资源穗部遗传多样性丰富,居群内多样性指数(H′)在0.784～1.652,平均多样性指数为1.396;居群总平均多样性指数为1.936,居群间表型分化系数为27.89%。14项穗部形态性状在居群内多样性、居群总多样性以及变异系数变化不完全一致,居群内H′最高的为旗叶与穗基部长度(1.648),居群总H′最高的为小穗长(2.054),变异系数最大的为第一颖芒长(58.932%),平均变异系数为31.22%,表明穗部形态性状均存在较大的变异,F检验差异极显著。引起居群形态差异的性状与种子产量密切相关,主要包括穗长、穗宽、第一颖芒长、第一外稃长、第一内稃长、第一外稃芒长、每穗轴小花数、第一颖长和第一颖宽。我国野生披碱草属牧草的多样性和分布格局是相关的,我国西北部是野生披碱草属牧草分布中心,也是披碱草属牧草的多样性中心。此外,提出了我国披碱草属牧草保护的策略。     

噬菌体复合制剂对肉鸡生长性能、免疫功能、肠道形态及肠道菌群的影响

本试验旨在研究噬菌体复合制剂对肉鸡生长性能、免疫功能、肠道形态及肠道菌群的影响。选取14 000只体重（850±45） g的37日龄青脚麻鸡,随机分为2组,分别是对照组（基础饲粮）和试验组（基础饲粮+109PFU/mL的噬菌体复合制剂）,每组7个重复,每个重复1 000只,试验期27 d。结果表明：1）与对照组相比,试验组的料重比下降2.81%(P>0.05)。2）与对照组相比,43日龄时,试验组肉鸡胸腺指数和脾脏指数分别增加16.80%(P<0.05)和7.14%(P>0.05);52日龄时,肉鸡法氏囊指数和胸腺指数分别增加8.55%(P>0.05)和7.37%(P>0.05);61日龄时,肉鸡法氏囊指数增加39.66%(P>0.05);43、52及61日龄时,试验组肉鸡血清免疫球蛋白G(IgG)含量分别增加15.85%(P>0.05)、29.54%(P>0.05)和1.45%(P>0.05)。3）与对照组相比,43、52及61日龄时,试验组肉鸡十二指肠、空肠、回肠绒毛高度/隐窝深度显著提高（P<0.05）。4）在门水平上,...     

Co-inoculation of selected nodule endophytic rhizobacterial strains with Rhizobium tropici promotes plant growth and controls damping off in common bean

Production of common bean(Phaseolus vulgaris)is limited by the occurrence of damping off(rhizoctoniosis),which is caused by the fungus Rhizoctonia solani.However,the co-inoculation of plant growth-promoting rhizobacteria(PGPR)involved in biological control along with diatomic nitrogen(N2)-fixing rhizobia can enhance N nutrition and increase production.In this context,finding microorganisms with synergistic effects that perform these two roles is of fundamental importance to ensure adequate yield levels.The aim of this study was to evaluate the effects of co-inoculation of nodule endophytic strains of the genera Bacillus,Paenibacillus,Burkholderia,and Pseudomonas with Rhizobium tropici CIAT 899,an N2-fixing rhizobial strain,on the biocontrol of damping off and growth promotion in common bean plants.Greenhouse experiments were conducted under axenic conditions using the common bean cultivar Pérola.The first experiment evaluated the potential of the 14 rhizobacterial strains,which were inoculated alone or in combination with CIAT 899,for the control of R.solani.The second experiment evaluated the ability of these 14 rhizobacterial strains to promote plant growth with three manners of N supply:co-inoculation with CIAT 899 at low mineral N supply(5.25 mg N mL^-1),low mineral N supply(5.25 mg N mL^-1),and high mineral N supply(52.5 mg N mL^-1).The use of rhizobacteria combined with rhizobia contributed in a synergistic manner to the promotion of growth and the control of damping off in the common bean.Co-inoculation of the strains UFLA 02-281/03-18(Pseudomonas sp.),UFLA 02-286(Bacillus sp.),and UFLA 04-227(Burkholderia fungorum)together with CIAT 899 effectively controlled damping off.For the common bean,mineral N supply can be replaced by the co-inoculation of CIAT 899 with plant growth-promoting strains UFLA 02-281/02-286/02-290/02-293.Nodule endophytes UFLA02-281/02-286 are promising for co-inoculation with CIAT 899 in the common bean,promoting synergy with rhizobial inoculation and protection against disease.