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排序方式: 共有109条查询结果,搜索用时 15 毫秒
1.
Vincenzo Montesano Donatella Negro Giulio Sarli Giuseppina Logozzo Pierluigi Spagnoletti Zeuli 《Genetic Resources and Crop Evolution》2012,59(5):701-716
A collecting expedition has been carried out in the inland areas of the Basilicata Region, South Italy. Each of the 14 selected municipalities was subdivided in three strata based on the geographical distribution of population and orography: 1. city boundaries, 2. group of country houses, 3. isolated farm houses. Five sites were selected within each stratum/town for a total of 185 sites. In 101 sites crop species germplasm was collected and the farmer-maintainers were interviewed. Altogether 350 accessions were collected, the most common species were Capsicum annuum, Lycopersicon esculentum and Phaseolus vulgaris. A significant number of landraces are still preserved on farm, mostly in isolated farm houses (average age of farmers: 60?years) adopting traditional farming systems but a number of young farmers were also present. Perspectives for on farm conservation and management are discussed and public economic support for “farmer-maintainers” proposed. 相似文献
2.
Irene Abbattista Gentile Lucia Ferraris Mario Sanguinetti Marinela Tiprigan Giuseppina Fisichella 《Pest management science》1992,34(3):297-301
The effect of some physical factors on the processes (hydrolysis, volatilisation) regulating the persistence of methyl bromide in fresh surface waters was investigated to complement previous research. It has been confirmed that electromagnetic radiation in the visible field has no influence on the hydrolysis kinetics of the fumigant. The removal of methyl bromide by volatilisation from stirred and ventilated solutions appears to be of greater importance in practice. It has been shown that under these conditions the half-life is directly proportional to the liquid depth. Without stirring volatilisation is greatly reduced, due to the low diffusivity of methyl bromide in the aqueous phase. 相似文献
3.
A case of preputial glomangioma in an 8-year-old Springer Spaniel dog is reported. The dog presented a solitary nodular mass, approximately 3.5 x 5.2 cm in diameter, located on the lateral wall of the prepuce at the base of the penis. Histologically, the excised tumour mass consisted of proliferating round epithelioid cells arranged in a cordon-like structure around small blood vessels. The neoplastic cells were positive for alpha smooth muscle actin and negative for cytokeratin, desmin, S-100 protein and neurone-specific enolase. Based on these pathologic features, the tumour was diagnosed as glomangioma, a neoplasm extremely rare in dogs. To our knowledge, this is the first report of preputial glomangioma in the dog. 相似文献
4.
Basile A Sorbo S Giordano S Ricciardi L Ferrara S Montesano D Castaldo Cobianchi R Vuotto ML Ferrara L 《Fitoterapia》2000,71(Z1):S110-S116
Following the extraction of Castanea sativa with an aqueous solution of sulfuric acid (pH 3.0), the ethyl acetate soluble fraction was tested for its antibacterial and allelopathic activity. The extract was shown to have pronounced antibacterial effects against seven of the eight strains of Gram-positive and Gram-negative bacteria used (MIC in the range of 64-256 microg/ml and MBC in the range of 256-512 microg/ml). The active fraction was analyzed by TLC and HPLC showing the presence of rutin, hesperidin, quercetin, apigenin, morin, naringin, galangin and kaempferol. Standards of the identified flavonoids were tested against the same bacterial strains. The highest activity was shown by quercetin, rutin and apigenin. The allelopathic effect was tested against Raphanus sativus seed germination. The extract, quercetin, rutin and apigenin caused a decrease in the percentage of seed germination and root and epicotyl growth. 相似文献
5.
6.
Characterization of dinucleotide and trinucleotide EST-derived microsatellites in the wheat genome 总被引:6,自引:0,他引:6
Over the past decade microsatellites or simple sequence repeats (SSRs) have attracted a considerable amount of attention from
researchers. The aim of the present paper was to analyse expressed sequence tag-derived SSR (EST-SSR) marker variability in
wheat and to investigate the relationships between the number and type of repeat units and the level of microsatellite polymorphism.
Two hundred and forty-one new EST-SSR markers available in a public database () were characterized in eight durum wheat cultivars (Svevo, Ciccio, Primadur, Duilio, Meridiano, Claudio, Latino, Messapia),
two accessions of Triticum turgidum var. dicoccoides (MG4343, MG29896), one accession of T. turgidum var. dicoccum (MG5323) and in the common wheat cv. Chinese Spring. Of these, 201 primer pairs (83.4%) amplified PCR products successfully,
while the remaining 40 (16.6%) failed to amplify any product. Of the EST-SSRs analysed, 45.2% of the primer pairs amplified
one or two PCR products. Multiple discrete PCR products were observed among both di- and trinucleotide EST-SSR markers (31.2
and 40.5%, respectively). Markers based on dinucleotide microsatellites were more polymorphic than those based on trinucleotide
SSRs in the 12 wheat genotypes tested (68.9 and 52.7%, respectively). An average of 2.5 alleles for dinucleotide and 2.0 alleles
for trinucleotide SSRs was observed. The data reported in the present work indicate the presence of a significant relationship
between motif sequence types and polymorphism. The primer set based on the AG repeat motif showed the lowest percentage of
polymorphism (55.0%), while the primer set based on the AC repeat motif showed t he highest percentage (85.0%). Among trinucleotide
SSRs, the AGG microsatellite markers showed the highest percentage of polymorphism (70.0%), and the ACG motif the lowest value
(25.0%). The characterization of these new EST-SSR markers and the results of our studyon the effect of repeat number and
type of motifs could have important applications in the genetic analysis of agronomically important traits, quantitative trait
locus discovery and marker-assisted selection. 相似文献
7.
Ranalli A Pollastri L Contento S Di Loreto G Iannucci E Lucera L Russi F 《Journal of agricultural and food chemistry》2002,50(13):3775-3779
The contents of triacylglycerols and diacylglycerols in three kinds of olive fruit oils (pulp, seed, and whole fruit) were determined. The fatty acid composition and the quality ratios 1,2-diacylglycerols/1,3-diacylglycerols and 1,2-diacylglycerols/total diacylglycerols were also assessed. Seven major Italian olive varieties were considered. Results of univariate statistical analyses indicated that the above analytical parameters (glyceridic ratios excepted) were effective in discriminating between pulp and seed oils. The seed oil fraction did not determine any change in the glyceridic indices and the acylglycerol or fatty acid composition concerning the whole fruit oil (mixture of pulp and seed oil fractions), the weight (%) of seed ( approximately 2%) being by far lower than the weight (%) of pulp ( approximately 85%) (fruit weight basis). Based on the data of triacylglycerol or fatty acid composition, and using appropriate parametric or nonparametric multivariate statistics, the genetic origins (olive variety) of the three fruit oil kinds were characterized. 相似文献
8.
Caruso C Candore G Colonna-Romano G Lio D Franceschi C 《Science (New York, N.Y.)》2005,307(5707):208-9; author reply 208-9
9.
Giuseppe Ferrara Ivana Cavoski Andrea Pacifico Luigi Tedone Donato Mondelli 《Scientia Horticulturae》2011
A recent increasing demand in Western countries for pomegranate products by consumers is especially supported for the nutritional and medicinal characteristics, due to the antioxidant properties of this fruit. Some studies have been published on the morphological and biochemical characteristics of pomegranate fruits in some Mediterranean countries, but little information is available about the genotypes present in Italy and in particular in Apulia (Southeastern region of Italy). This study (2008–2009) evaluated morpho-pomological and chemical parameters of eight pomegranate genotypes localized in private small orchards. Significant differences were observed among the pomegranate genotypes for many of the parameters investigated. In particular, fruit weight ranged from 168.9 g (SouMol) to 574.9 g (SouOst), °Brix from 14.7 (ComTri) to 18.0 (SouMol), titratable acidity from 5.4 (ComMol) to 25.0 (SouTri) g/L. SouMol showed the highest polyphenols (97.1 mg/L) and vitamin C (236.3 mg/L) contents. Oil content of the seeds was between 5.90% and 10.30%, no differences have been observed for the fatty acid composition with conjugated linoleic acid (CLA) isomers as the most abundant fraction (81.23%). Considering all the evaluated parameters, and especially those referring to the organoleptic characteristics and antioxidants content, it must be stated that the best genotypes worthy to be considered from agricultural and industrial points of view were AdeSgi for fresh market and SouOst for the juice industry. 相似文献
10.
Data both from the literature and from our own research are reported on the Hg levels in the soil, waters, sediment , atmosphere and some plants of the mineralized Monte Amiata region (Italy) with the aim of evidencing the interactions between the different environmental compartments. The presence of cinnabar in the soil affects the whole area, particularly near the mines, roasted cinnabar deposits and at the steam jets used for the geothermal power plants. Soil degassing represents the main source of atmospheric Hg which shows a concentration range of 5 to 200 ng m?3. Vegetables display high Hg levels (0.06 to 9.80 µg g?1) especially in the leaves. The aqueous transport of dissolved mercury is of no importance; suspended particulate matter, however, is able to carry a significant load of Hg. 相似文献