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Jergensen, C. B., J. S. Agerholm, J. Pedersen and P. D. Thomsen: Bovine leukocyte adhesion deficiency in Danish Holstein-Friesian Cattle I. PCR screening and allele frequency estimation. Acta vet. Scand. 1993,34,231-236.–A screening program for bovine leukocyte adhesion deficiency (BLAD) in Danish Holstein-Friesian cattle has been initiated. During the first months 1611 animals were tested by a PCR based assay. Of these animals 1256, 346, and 8 were assigned normal, BLAD carriers, and BLAD affected animals, respectively One bull, born as a co-twin, showed weak reaction for the BLAD allele on DNA isolated from leukocytes, but a normal genotype on DNA isolated from semen. Chromosome analysis showed that this bull was a blood chimaera. Estimation of the BLAD allele frequency upon the PCR test results showed that around 450 Danish calves born in 1991 might have been affected with the recessive disorder. 相似文献
3.
The prevalence of E. coli, coliforms and Vibrio-like-organisms has been studied at four different sites along the east coast of Zealand. High values were registered in sediment taken from the water-line and at a depth of one meter. Generally there exist significant differences between the bacterial counts of water and sediment, and it is obvious that the greatest concentration is in the waterline sediment. The epidemiological and hygienic consequences of these facts are discussed. 相似文献
4.
Deanna L. Funnell Christopher B. Lawrence Jeffrey F. Pedersen Christopher L. Schardl 《Physiological and Molecular Plant Pathology》2004,65(6):285-296
Systemic acquired resistance (SAR) is induced following inoculation of Peronospora tabacina sporangia into the stems of Nicotiana tabacum plants highly susceptible to the pathogen. Previous results have shown that accumulation of acidic β-1,3-glucanases (PR-2's) following induction of SAR by P. tabacina may contribute to resistance to P. tabacina. We showed that up-regulation of the PR-2 gene, PR-2d, following stem inoculation with P. tabacina, is associated with SAR. Studies using plants transformed with GUS constructs containing the full length promoter from PR-2d or promoter deletions, provided evidence that a previously characterized regulatory element that is involved in response to salicylic acid (SA), may be involved in regulation of PR-2d following induction of SAR with P. tabacina. This work provides evidence that regulation of PR-2 genes during P. tabacina-induced SAR may be similar to regulation of these genes during infection of N-gene tobacco by TMV or following exogenous application of SA, and provides further support for the role of SA in regulation of genes during P. tabacina-induced SAR. 相似文献
5.
Kaarina Matilainen Ismo Strandén Gert Pedersen Aamand Esa A. Mäntysaari 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2018,135(5):337-348
Joint Nordic (Denmark, Finland, Sweden) genetic evaluation of female fertility is currently based on the multiple trait multilactation animal model (BLUP). Here, single step genomic model (ssGBLUP) was applied for the Nordic Red dairy cattle fertility evaluation. The 11 traits comprised of nonreturn rate and days from first to last insemination in heifers and first three parities, and days from calving to first insemination in the first three parities. Traits had low heritabilities (0.015–0.04), but moderately high genetic correlations between the parities (0.60–0.88). Phenotypic data included 4,226,715 animals with records and pedigree 5,445,392 animals. Unknown parents were assigned into 332 phantom parent groups (PPG). In mixed model equations animals were associated with PPG effects through the pedigree or both the pedigree and genomic information. Genotype information of 46,914 SNPs was available for 33,969 animals in the pedigree. When PPG used pedigree information only, BLUP converged after 2,420 iterations whereas the ssGBLUP evaluation needed over ten thousand iterations. When the PPG effects were solved accounting both the pedigree and the genomic information, the ssGBLUP model converged after 2,406 iterations. Also, with the latter model breeding values by ssGBLUP and BLUP became more consistent and genetic trends followed each other well. Models were validated using forward prediction of the young bulls. Reliabilities and variance inflation of predicted genomic breeding values (values for parent averages in brackets) for the 11 traits ranged 0.22–0.31 (0.10–0.27) and 0.81–0.95 (0.83–1.06), respectively. The ssGBLUP model gave always higher validation reliabilities than BLUP, but largest increases were for the cow fertility traits. 相似文献
6.
赤霉菌含毒素代谢物对小黑麦愈伤组织和胚芽生长的影响 总被引:2,自引:2,他引:2
选用对赤霉病抗性水平不同的品种(系)和6个赤霉病菌株,采用双层培养技术,研究了赤霉菌含毒素代谢物对小黑麦愈伤组织和幼苗胚芽长度的影响。结果表明,和无毒素处理的对照相比,赤霉菌含毒素代谢物对愈伤组织生长有极显著或显著的抑制作用,而对幼苗胚芽生长有极显著或显著的刺激作用,田间对赤霉病(FHB)抗性差的品种分别受抑制程度最重和对刺激反应最迟纯。品种间、菌株间存在着极显著或显著差异,在幼苗胚芽长度上,发现品种和菌株间存在极显著的互作效应。分析后认为,在多菌株接种下,品种平均相对生长量(和对照相比)是一个更加可靠的抗性鉴定指标。 相似文献
7.
Breeding Restrictions Decrease the Prevalence of Myxomatous Mitral Valve Disease in Cavalier King Charles Spaniels over an 8‐ to 10‐Year Period
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8.
This paper describes the development of a systems based model to characterise farmers’ decision-making process in information-intensive practices, and its evaluation in the context of Precision Agriculture (PA). A participative methodology was developed in which farm managers decomposed their process of decision-making into brief decision statements along with associated information requirements. The methodology was first developed on a university research farm in Denmark and further revised during testing on a number of research and commercial farms in Indiana, USA. Twenty-one decision-analysis factors were identified to characterise a farm manager’s decision-making process. Then, a general data flow diagram (DFD) was constructed that describes the information flows “from data to decision”. Illustrative examples of the model in the form of DFDs are presented for a strategic, a tactical and an operational decision. The model was validated for a range of decisions related to operations by three university farm managers and by five commercial farmers practicing PA for cereal, corn and soybean production in Denmark and in Indiana, USA. 相似文献
9.
Atle Foss Albert K. Imsland Erik Vikingstad Sigurd O. Stefansson Birgitta Norberg Skjalg Pedersen Trond Sandvik Bjrn Roth 《Aquaculture (Amsterdam, Netherlands)》2009,290(3-4):304-310
A group of juvenile Atlantic halibut (mean, SD, 199.5 g, 44.7) was split into two experimental groups: one group was fed in excess twice a day and later five days a week (i.e. Control group), and the other group was starved for 5 weeks and then subsequently re-fed for 10 weeks (i.e. 5/10 starvation/re-fed group). This treatment was repeated for three years until the fish were harvested (mean final weight, 4.4 kg). A size +specific compensatory response was seen; partial compensation was observed in halibut weighing less than 500 g whereas full recovery growth was seen in halibut over 2 kg. Food consumption and feed conversion efficiency was measured during two full 5/10 starvation/re-fed cycles. The Control group consumed 86% more food in the measurement period, whereas the FCE 40% higher in the 5/10 starvation/re-fed group (FCE = 0.95) compared to the Control group (FCE = 0.68). In males lower gonadosomatic index (2.2 vs. 3.0) and plasma 11-ketotestosterone (0.22 vs. 1.26 ng ml− 1) levels in the 5/10 starvation/re-fed group were observed during late winter and spring of 2006 (age 3 years) indicating higher age at 1st maturity. Starvation had a significant effect for improving the quality as starved fish had higher muscle pH (6.4 vs. 6.2), less gaping (0.1 vs. 0.3) and harder texture (61.4 vs. 57.6 N). Overall, the rearing on a repeated 5/10 starvation/re-fed regime for 3 years led to full growth compensation, higher feed conversion efficiency, lower male maturation and improved flesh quality. 相似文献
10.
Albert K Imsland Bjørn Roth Atle Foss Erik Vikingstad Sigurd O Stefansson Skjalg Pedersen Trond Sandvik & Birgitta Norberg 《Aquaculture Research》2009,40(11):1260-1269
The aim of this study was to investigate the effect of continuous light at different stages during the production cycle of Atlantic halibut Hippoglossus hippoglossus L. on growth, age at first maturity, endocrine parameters and flesh quality. A group of juvenile halibut [mean (SD), initial weight 191.3 g (44.7)] was reared in indoor tanks under ambient temperature conditions for 38 months until harvesting (mean final weight, 4.6 kg). The entire photoperiod experiment was divided into four phases, where the fish in each phase were exposed to either natural photoperiod (62°33'N) or continuous light (L). Thus, the following five photoperiod combinations were tested: (a) Control group (NNNN), (b) Group 2A (NLNN), (c) Group 2B (NNLN), (d) Group 2C (NNNL) and (e) Production group (LNNN). Exposure to continuous light stimulated growth, and the final mean weights of Groups 2A and 2B were 23% and 11% higher than those of the Control group (NNNN). The final plasma 11-ketotestosteron levels were lower in Groups 2A (2.94 ng mL−1 ) and 2B (2.46 ng mL−1 ) compared with the Control (5.29 ng mL−1 ), Group 2C (5.09 ng mL−1 ) and the Production group (4.78 ng mL−1 ) during spring 2007 (age 4 years), indicating higher age at first maturity in Groups 2A and 2B. Photoperiod regime had only a minor, and transient, effect on flesh-quality traits of the fish, whereas a significant seasonal effect was seen with a tendency towards increased gaping, lower pH, lower hardness and lower shear force in July compared with December and March. 相似文献