Gonadotrophin-releasing hormone treatment in cattle: a meta-analysis of the effects on conception at the time of insemination

SUMMARY Data from 40 trials described in 27 published papers were analysed by meta-analysis (pooling of data from numerous trials into a single analysis) to evaluate the effects of injecting gonadotrophin-releasing hormone (GnRH) In cattle at the time of Insemination on the risk of pregnancy. A total of 19 019 cows were involved In these studies. Analyses were stratified by trial and by the effects of dose of GnRH, type of GnRH and Insemination number (first, second or third and greater). Mantel-Haenszel analysis revealed that use of GnRH at Insemination significantly Increased the overall risk of pregnancy by 12.5% In treated cows (P < 0.05). However, Increases In risk of pregnancy were greatest (22.5%) when repeat breeders were treated. Results for use of GnRH or analogue at first service were similar, with Increased risks of pregnancy in treated cows of 5.2 and 8.0%, respectively. The risk of pregnancy tended to be higher (11.1%) when the dose of GnRH was 250 μg and when used at second Insemination (9.9%). The analyses demonstrated that while treatment at Insemination with GnRH and GnRH analogues Increased conception rates In dairy cattle, some variation In study results was attributable to the number of Inseminations after calving at which GnRH Is administered. Further efforts should be made to determine characteristics of populations of cows that have good fertility responses to GnRH and to determine the mode of action of GnRH in Increasing fertility. Meta-analysis proved to be a useful technique for evaluating the apparently conflicting results from trials and in evaluating the effect of factors such as Insemination number, dose and type of GnRH on responses to treatment.     

Estimates of genetic variance and variance of predicted genetic merits using pedigree or genomic relationship matrices in six Brown Swiss cattle populations for different traits

The amount of variance captured in genetic estimations may depend on whether a pedigree‐based or genomic relationship matrix is used. The purpose of this study was to investigate the genetic variance as well as the variance of predicted genetic merits (PGM) using pedigree‐based or genomic relationship matrices in Brown Swiss cattle. We examined a range of traits in six populations amounting to 173 population‐trait combinations. A main aim was to determine how using different relationship matrices affect variance estimation. We calculated ratios between different types of estimates and analysed the impact of trait heritability and population size. The genetic variances estimated by REML using a genomic relationship matrix were always smaller than the variances that were similarly estimated using a pedigree‐based relationship matrix. The variances from the genomic relationship matrix became closer to estimates from a pedigree relationship matrix as heritability and population size increased. In contrast, variances of predicted genetic merits obtained using a genomic relationship matrix were mostly larger than variances of genetic merit predicted using pedigree‐based relationship matrix. The ratio of the genomic to pedigree‐based PGM variances decreased as heritability and population size rose. The increased variance among predicted genetic merits is important for animal breeding because this is one of the factors influencing genetic progress.     

Congenital hypothyroidism in Scottish Deerhound puppies

Two Scottish Deerhound puppies had clinical and pathological features consistent with the diagnosis of congenital non-goitrous hypothyroidism. They were from separate litters, but were the progeny of the same sire and dam. The puppies were smaller, had shorter limbs and shorter, broader heads than their littermates. They also had histories of weakness, difficulty in walking and somnolence. A characteristic radiographic feature was the absence of epiphyseal growth centres. Both had depressed serum thyroxine (T4) levels and one did not respond to exogenous thyroid stimulating hormone. On necropsy, the thyroid glands were small, the follicles varied in size and contained little or no colloid. The adenohypophysis contained many cells with markedly vacuolated cytoplasm. It is suggested that the clinicopathological pattern is the result of a primary thyroid abnormality. Possible mechanisms include either primary thyroid hypoplasia or an unresponsiveness to thyroid stimulating hormone.     

The chemistry of water on alumina surfaces: reaction dynamics from first principles

Aluminas and their surface chemistry play a vital role in many areas of modern technology. The behavior of adsorbed water is particularly important and poorly understood. Simulations of hydrated alpha-alumina (0001) surfaces with ab initio molecular dynamics elucidate many aspects of this problem, especially the complex dynamics of water dissociation and related surface reactions. At low water coverage, free energy profiles established that molecularly adsorbed water is metastable and dissociates readily, even in the absence of defects, by a kinetically preferred pathway. Observations at higher water coverage revealed rapid dissociation and unanticipated collective effects, including water-catalyzed dissociation and proton transfer reactions between adsorbed water and hydroxide. The results provide a consistent interpretation of the measured coverage dependence of water heats of adsorption, hydroxyl vibrational spectra, and other experiments.     

Genetic trends for fertility,udder health and protein yield in Swedish red cattle estimated with different models

The aim of this study was to estimate and compare genetic trends in Swedish Red cattle using a full multiple-trait (MT) model and trait-group-wise models for female fertility, udder health and protein yield. Field data for maiden heifers from 1989 and cows with a first and second lactation between 1990 and 2007 were included. (Co)variance components were estimated prior to prediction of breeding values. The estimated genetic trends were clearly favourable for protein yield and udder conformation, and in most cases neutral to favourable for clinical mastitis and calving to first insemination. In maiden heifers, the trends were neutral for number of inseminations per service period. Unfavourable genetic trends were estimated for number of inseminations in the first two lactations, but the trends seemed less unfavourable from evaluations within trait groups compared with when using the full MT model. Excluding maiden heifer data affected genetic trends less than using trait-group-wise analyses instead of a full MT model. Unfavourable genetic trends in functional traits may be missed unless the traits are evaluated in a MT model including traits under strong selection.     

A survey of bone integrity from cull sows in Ireland

The objective of this study was to examine the differences in sow metacarpal properties through various parities and to compare the incidence of locomotory problems between stalled and loose-housed sows. Metacarpals (n = 110) of sows from six farms were collected at slaughter and stored at -20°C. Bones from one forelimb of sows in stalls (n = 36) and loose-housed (n = 20) were collected and their articular surfaces examined for Osteochondrosis Dissecans (OCD), these sows were also scored for lameness pre-slaughter. Metacarpals were CT scanned for cross sectional area and moment of inertia. Cylindrical sections from the diaphysis were used for mechanical testing and calculating bone strength indices. The results show there was little change in bone mineral status throughout the range of parities examined and overall metacarpal integrity was not compromised by multiple production cycles. There were no differences detected in the incidence of lameness or joint pathology between housing systems.     

Genetic variation and genetic trends in hip and elbow dysplasia in Swedish Rottweiler and Bernese Mountain Dog

The aim of this study was to estimate genetic parameters and genetic trends for hip (HD) and elbow dysplasia (ED) in Swedish Rottweiler (RW) and Bernese Mountain Dog (BMD). Analyses were based on screening results of hip status for 14 693 RW and 8221 BMD and elbow status for 11 891 RW and 7963 BMD, as well as pedigree data for 16 614 RW and 9835 BMD, recorded by the Swedish Kennel Club. Components of (co)variance and breeding values were obtained with a mixed linear animal model. The model included the fixed effects of sex, birth month, age at screening and a combined random effect of clinic and year of examination. The need to include genetic groups for phantom parents in the model was evaluated by comparison of two different models: with and without genetic groups. Estimated heritabilities for HD and ED were between 0.34 and 0.42. The genetic correlation between the traits was weak and positive for RW (r_g = 0.23 ± 0.05) and not different from zero for BMD (r_g = 0.06 ± 0.06). F‐statistics of the genetic group effects were not significant, implying that genetic groups do not need to be included in the model. Genetic trends indicated a genetic improvement in both traits. However, a faster genetic progress is expected if selection is based on predicted breeding values rather than phenotype. Based on the results, a statistical model for routine prediction of breeding values for HD and ED in Swedish dogs was suggested.     

Simultaneous genetic evaluation of simulated mastitis susceptibility and recovery ability using a bivariate threshold sire model

The aim of this study was to develop a new approach for joint genetic evaluation of mastitis and recovery. Two mastitis incidences (0.28 and 0.95) measured via somatic cell count and three between traits genetic correlations (0.0, 0.2, and ?0.2) were simulated for daughter group sizes of 60 and 240. A transition model was applied to model transitions between healthy and disease state. The RJMC package in DMU was used to estimate (co)variances. Heritabilities were consistent with the simulated value (0.039) for susceptibility and a bit upward biased for recovery. Estimates of genetic correlations were ?0.055, 0.205, and ?0.192 for the simulated values of 0.0, 0.2, and ?0.2, respectively. For daughter group size of 60, accuracies of sire EBV ranged from 0.56 to 0.69 for mastitis and from 0.26 to 0.48 for recovery. The study demonstrated that both traits can be modeled jointly and simulated correlations could be correctly reproduced.     

Sperm Morphology Assessment in Captive Neotropical Primates

The main objective of this study was to evaluate sperm morphology in four neotropical primate species to compare the sperm morphological traits and the sperm morphometric parameters as a basis for establishing normative sperm standards for each species. Data from 80 ejaculates collected from four primate species, Callithrix jacchus, Callimico goeldii, Alouatta caraya and Ateles geoffroyi, were analysed for detection of sperm morphological alterations using subjective World Health Organization (WHO‐2010) standards and Sperm Deformity Index (SDI) criteria, objective computer‐assisted sperm morphometry analysis (CASMA) and subpopulation sperm determination (SSD) methods. There were multiple differences (p < 0.01) observed among primate species in values obtained from WHO‐2010, SDI, CASMA and SSD sperm analysis methods. In addition, multiple significant positive and negative correlations were observed between the sperm morphological traits (SDI, Sperm Deformity Index Head Defects, Sperm Deformity Index Midpiece Defects, Sperm Deformity Index Tail Defects, Normal Sperm, Head Defects, Midpiece Defects and Tail Defects) and the sperm morphometric parameters (SSD, Area (A), Perimeter (P), Length (L), Width (W), Ellipticity, Elongation and Rugosity) (p ≤ 0.046). In conclusion, our findings using different evaluation methods indicate that pronounced sperm morphological variation exists among these four neotropical primate species. Because of the strong relationship observed among morphological and morphometric parameters, these results suggest that application of objective analysis methods could substantially improve the reliability of comparative studies and help to establish valid normative sperm values for neotropical primates.