Assessment of genetic relationships among 29 introduced and 49 local sweet cherry accessions in Turkey using AFLP and SSR markers

Summary

The characterisation of sweet cherry (Prunus avium L.) genetic resources in Turkey may help to increase their use in breeding programmes worldwide, as Turkey is the centre of origin of sweet cherry. Amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers were therefore used to analyse genetic diversity among a total of 78 local and introduced sweet cherry cultivars. Four AFLP primer combinations, and six SSR primer pairs for sweet cherry were used for genetic diversity analysis. A genetic similarity matrix was calculated using the combined data from AFLP and SSR analyses with simple matching coefficient. Genetic similarities among the sweet cherry genotypes studied were higher than 42%. No two accessions had an identical AFLP and SSR marker profile, indicating that all 78 genotypes were unique. An UPGMA dendrogram, based on the similarity matrix, revealed 18 separate Groups at or above the 70% similarity level. While some Groups consisted of both introduced and local genotypes, other Groups had only local genotypes. This result suggests that there was broad genetic diversity among the local Turkish sweet cherry genotypes, which was not present in the introduced sweet cherry accessions. The genetic variation present in local Turkish sweet cherry genotypes may be useful for future breeding programmes. We found that the use of both SSR and AFLP marker systems was effective for distinguishing between genetically-close sweet cherry genotypes. These marker systems can be used to complement pomological and morphological markers during the characterisation and identification of sweet cherry genotypes.     

Sprinkling coarse marble powder during the afternoon, toward the end of the laying period, may increase eggshell quality

The aim of this study was to investigate the effects of time, particle size (PS) and level of calcium supplementation above the requirements toward the end of the laying period on egg production and eggshell quality. A total of 675 Isa-Brown layer hens were distributed in a completely randomized factorial arrangement of 3×3×3, using three sprinkling times (08.00, 12.00, 16.00 hours), PC (mash, granular and large size) and amounts (0 g control, 0.75 g/day/h, 1.50 g/day/h) of marble sprinkling as a calcium source. All hens were fed a commercial layer diet from 55 to 67 weeks of age. Sprinkling of 1.50 g marble reduced ( P  < 0.05) egg production compared to 0.75 g marble, and feed efficiency (FE) compared to that of 0 and 0.75 g marble. Sprinkling of 0.75 and 1.50 g marble increased ( P  < 0.01) eggshell weight, shell strength and shell thickness compared to that of 0 g marble. The interaction of sprinkling time-by-PS-by–quantity of marble was significant for egg production ( P  < 0.05), FE and shell strength ( P  < 0.01). Results showed that sprinkling of coarse marble powder to complete diet increased shell strength without reduction in egg production and FE.     

Potential of Gonium spp. in Synthetic Reactive Dye Removal, Possible Role of Laccases and Stimulation by Triacontanol Hormone

In this study, Gonium sp. was investigated for possible usage in dye-containing wastewater treatment. Trials were performed in media including triacontanol hormone, Reactive Orange 14, Reactive Red 120, Reactive Black 5, Remazol Brilliant Blue R (RBBR), and also hormone against the controls. Algae could remove RBBR with the highest dye removal percentage (56%) among the tested dyes. The optimum pH was 9 in removing 50 mg L^?1 RBBR at a dye removal percentage of 47.1%. The role of laccase activity of Gonium sp. was also investigated. This first attempt in the literature showed the involvement of the enzyme in the algal growth and bioremoval process. In the presence of the plant growth hormone in the culture, the activity showed a steady and significant increase up to nearly sixfold between 5th and 14th days of incubation.     

Ecology of tertiary relict endemic Liquidambar orientalis Mill. forests

The oriental sweetgum, Liquidambar orientalis Mill. is a tertiary period relict endemic taxon of the east Mediterranean. The flat deep hydromorphic soils rich in surface waters during summer months are the most productive sites for the dense stands of L. orientalis. It is mainly distributed on sandy soils, with an alkaline pH. The CaCO₃ content in the soils show a medium value and the percentage of total salt content is very low, but organic matter content is higher. The monoecious trees are on an average 15–20 m tall and form dense forests confined to floodplains, valleys and along streams and in a few dry habitats. The plant shows a typical leaf dimorphism with sparsely lobed leaves at the top and many lobed leaves on the lower parts. Fresh seeds germinate well and the percentage varies from 60 to 80. A syntaxonomical synthesis of these forests was carried out. All associations of L. orientalis including the newly defined Querco ilicis-Liquidambaretum orientalis association have been evaluated within the alliance Platanion orientalis Karpati & Karpati 1961. This alliance is included in the order Platanetalia orientalis Knapp 1959, and class Alno-populetea Knapp 1959.     

Bartonella henselae seroprevalence in cattle breeders and veterinarians in the rural areas of Aydin and Denizli, Turkey

Bartonella henselae infections are usually detected among people who have close contact with animals. Veterinarians and cattle breeders, in particular, are considered as the risk groups for B. henselae infections. In this study, the seroprevalence of antibodies to B. henselae was investigated in these two groups of subjects in the two cities of Aydin and Denizli, which are located in the same region in the southwest of Turkey. Total antibodies to B. henselae were evaluated by indirect immunofluorescence assay in serum samples taken from 63 cattle breeders and 27 veterinarians. Twenty samples (22.2%) were found to react on 1/64 titre with B. henselae antigens. Bartonella henselae seroprevalence was found to be significantly related to age (P = 0.033) and higher in those living in Aydin (P = 0.047). Age was the only independent factor in multivariate analysis (P = 0.008). Seroprevalence was found to be 2‐fold higher in those people who had had tick contact (P = 0.093). In conclusion, the physicians in the region should consider B. henselae infection among veterinarians and breeders in their differential diagnosis list of fever of unknown origin.     

Expression and localisation of Bcl-2 and Bax proteins in developing rat ovary

Bcl-2 and Bax proteins localised mainly in granulosa cells. Primordial and primary follicles of new born rat ovary showed an intensive nuclear staining for Bax but faint staining for Bcl-2. In terms of staining intensity, no remarkable difference was observed within the same stage of developing follicle. Compared to new born rats, granulosa cells of adult and one month old rat ovary showed an increased staining both for Bcl-2 and Bax proteins. No staining was observed in primordial follicles of one month old and adult rats. However, granulosa cells of primary follicles, granulosa cells and theca cells in tertiary follicles of adult rat ovary also showed a strong staining for Bcl-2 and Bax proteins. Oocytes of follicles from different developmental stages revealed an apparent staining both for Bcl-2 and Bax proteins. However, in the more mature follicles oocytes stained more intensively. In developing corpus luteum a remarkable staining was observed for Bax. However, the staining was more prominent in regressing corpus luteum. Contrary to this, Bcl-2 stained the luteal cells in developing corpus luteum strongly, while in the fully developed corpus luteum no staining for Bcl-2 was observed. In conclusion, there was an apparent relation between the expression of the apoptosis regulating protein Bcl-2 and Bax and follicular development. Thus, during the follicular development Bcl-2 and Bax may be involved in granulosa cell demise in rat ovary. Furthermore, increased levels of Bax and decreased levels of Bcl-2 in the fully developed corpus luteum suggest that Bax plays a role in apoptosis of luteal cells in rat ovary.     

Cystocele and rectal prolapse in a female dog

A case of cystocele and prolapsed rectum is reported. The urinary bladder and rectum were repositioned and fixed by cystopexy, colposuspension, and colopexy concurrently. There was no recurrence after 3 months. This is the first report to describe cystocele in a young female dog never having been pregnant.     

A new population of very high energy gamma-ray sources in the Milky Way

Very high energy gamma-rays probe the long-standing mystery of the origin of cosmic rays. Produced in the interactions of accelerated particles in astrophysical objects, they can be used to image cosmic particle accelerators. A first sensitive survey of the inner part of the Milky Way with the High Energy Stereoscopic System (HESS) reveals a population of eight previously unknown firmly detected sources of very high energy gamma-rays. At least two have no known radio or x-ray counterpart and may be representative of a new class of "dark" nucleonic cosmic ray sources.