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1.
Platelet aggregation in healthy and sick cats after adding various aggregating agents is described. Feline platelets aggregate irreversibly in response to 0.15-1.0 micrograms/ml collagen, 1 microM ADP, 0.3 IU/ml test-thrombin and 0.71 NIH/ml Topostasin. Epinephrine, ristocetin and kaolin failed to cause aggregation. The aggregation function was decreased in a cat with liver damage and icterus; in 2 cats with uremia platelet aggregation was normal. Acetylsalicylic acid (ASA) (10 mg/kg iv) inhibits platelet aggregation in the presence of collagen in low concentrations; high concentrations of collagen succeeded in inducing platelet aggregation. 相似文献
2.
Matthew M. Nelson Ben D. Mooney Peter D. Nichols Charles E Phleger Greg G. Smith Piers Hart Arthur J. Ritar 《Journal of the World Aquaculture Society》2002,33(2):146-157
The lipid class and fatty acid (FA) composition of juvenile Artemia fed continuously on four diets—the microalga Tetraselmis suecica , a mix of oat bran-wheat germ-lecithin (OWL), OwL-eicosapentaenoic acid (EPA), and OWL-EPA-arachidonic acid (AA)—were examined over a 9-d experiment in an attempt to approximate the FA profile of phyllosoma larvae of wild southern rock lobster Jasus edwardrii . The main difference in lipid class composition of Artemia fed the four diets was the relative level of polar lipid (PL) and triacylglycerol (TAG). By day 9, the algal-fed Artemia were highest in PL (95% of total lipid) and lowest in TAG (2%), whereas the remaining diets resulted in Artemia with 16–30% PL and 41–82% TAG. After 2 d, the relative FA composition of all Artemia treatments closely reflected those of the diets, with no marked change after further feeding (to day 9). In terms of the content of essential polyunsaturated fatty acids (PUFA), by day 5 Artemia fed: 1) with the algal diet contained 7 mg/g FA dry mass (0.3% DHA, 6.3% EPA, 3.4% AA of total FA); 2) with the OWL diet contained 3 mg/g (0.3% DHA, 0.9% EPA, 0.7% AA); 3) with the OWL-EPA diet contained 55 mg/g (6.2% DHA, 11.6% EPA, 1.1% AA); and 4) with the OWL-EPA-AA contained 83 mg/g (3.8% DHA, 7.5% EPA, 17.4% AA). The PUFA profiles of Artemia using the OWL-oil diets were similar to wild rock lobster phyllmmata, although levels of doco-sahexaenoic acid (DHA) were lower (10% DHA) than in J. edwardsii larvae. On the basis of PUFA composition data alone, the results suggest the suitability of the OWL-oil mixed diets for consideration for feeding to Artemia used in the culture of southern rock lobster larvae, particularly if the level of DHA can be further enhanced. 相似文献
3.
The phosphorylation, dissociation, and bimolecular reaction constants were determined for several members of a series of substituted O-ethyl O-phenyl S-n-propyl phosphorothioates in the presence of substrate. The data obtained are discussed in relation to electronegativity of substituents and in vivo toxicity. 相似文献
4.
Detection of Colletotrichum coccodes from soil and potato tubers by conventional and quantitative real-time PCR 总被引:4,自引:1,他引:4
Colletotrichum coccodes is the causal agent of the potato blemish disease black dot. Two PCR primer sets were designed to sequences of the ribosomal internal transcribed spacer (ITS1 and ITS2) regions for use in a nested PCR. The genus-specific outer primers (Cc1F1/Cc2R1) were designed to regions common to Colletotrichum spp., and the species-specific nested primers (Cc1NF1/Cc2NR1) were designed to sequences unique to C . coccodes . The primer sets amplified single products of 447 bp (Cc1F1/Cc2R1) and 349 bp (Cc1NF1/Cc2NR1) with DNA extracted from 33 European and North American isolates of C. coccodes. The specificity of primers Cc1NF1/Cc2NR1 was confirmed by the absence of amplified product with DNA of other species representing the six phylogenetic groups of the genus Colletotrichum and 46 other eukaryotic and prokaryotic plant pathogenic species. A rapid procedure for the direct extraction of DNA from soil and potato tubers was used to verify the PCR assay for detecting C. coccodes in environmental samples. The limit of sensitivity of PCR for the specific detection of C. coccodes when inoculum was added to soils was 3·0 spores per g, or the equivalent of 0·06 microsclerotia per g soil, the lowest level of inoculum tested. Colletotrichum coccodes was also detected by PCR in naturally infested soil and from both potato peel and peel extract from infected and apparently healthy tubers. Specific primers and a TaqMan fluorogenic probe were designed to perform quantitative real-time (TaqMan) PCR to obtain the same levels of sensitivity for detection of C. coccodes in soil and tubers during a first-round PCR as with conventional nested PCR and gel electrophoresis. This rapid and quantitative PCR diagnostic assay allows an accurate estimation of tuber and soil contamination by C. coccodes . 相似文献
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Effect of Age,Season, Body Condition,and Endocrine Status on Serum Free Cortisol Fraction and Insulin Concentration in Horses
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9.
An orbital sialocele developed in a dog following enucleation for protracted glaucoma. This eye had historically been treated for keratoconjunctivitis sicca by parotid duct transposition approximately 5 years previously, and the duct was ligated distally at the time of enucleation. One month following enucleation, the dog presented with a fluctuant conical-shaped swelling ventrolateral to the orbital socket. Surgical exploration revealed a dilated, fibrotic distal portion of the previously transposed parotid duct, and saliva, within the enucleated orbit. The distal portion of the duct and saliva-containing tissues from within the orbit were excised. The remaining proximal normal portion of the parotid duct was re-routed into the oral cavity. Clinicopathologic and histologic examination of the excised orbital contents and dilated portion of duct revealed a sterile sample of saliva and moderate chronic periductal fibrosis. At a 6-month re-evaluation there was no evidence of recurrence of the sialocele, and the parotid duct was functional. 相似文献
10.
Growth overfishing in the brown shrimp fishery of Texas, Louisiana, and adjoining Gulf of Mexico EEZ
Growth overfishing in the brown shrimp, Farfantepenaeus aztecus, fishery in inshore (estuarine) and offshore (Gulf of Mexico) territorial waters of Texas and Louisiana, and adjoining waters of the United States’ (U.S.) Exclusive Economic Zone (EEZ), and its potentially detrimental economic consequences to the harvesting sector, have not been among major concerns of Federal and State shrimp management agencies. Three possible reasons include (1) environmentally influenced variations in recruitment that cause wide fluctuations in annual landings, which tend to obscure effects of fishing, (2) competition between inshore and offshore components of the harvesting sector, and (3) partitioning of management jurisdiction among a Federal council and two State agencies. Wide variations in landings led to beliefs that high levels of fishing mortality were tolerable and recruitment overfishing was of no major concern. This encouraged somewhat laissez-faire management approaches that allowed fishing effort to increase over the years.Our objectives were to determine whether growth overfishing occurred in this fishery during 1960–2006, and whether and how decreases in size of shrimp within the landings, in response to increases in fishing effort, affected inflation-adjusted annual (calendar year) ex-vessel value of the landings, i.e., their value to the harvesting sector. Growth overfishing occurred in the early 1990s, and then abated as fishing effort declined due to rising fuel costs and competition from imported shrimp. However, inflation-adjusted annual ex-vessel value of the landings peaked in 1985, prior to growth overfishing.Management actions implemented in 2001 for Texas’ territorial waters, and in the EEZ off Texas and Louisiana in 2006, should limit future fleet expansion and increases in fishing effort, thereby reducing the chances of growth overfishing and its potentially detrimental economic impacts on the harvesting sector. Growth overfishing should be included among the guidelines for future management of this brown shrimp fishery. 相似文献