The fungal pathogenPhytophthora infestans is the causal organism of late blight, one of the most devastating diseases of potato. In the past, various aspects of the potato-P. infestans interaction have been studied extensively. In this paper we briefly review the current knowledge of the molecular events associated with the interaction and in addition we discuss a new approach for analyzing the molecular basis of pathogenicity ofP. infestans. 相似文献
AIM: To investigate a possible interaction between lolitrem B and ergovaline by comparing the incidence and severity of ryegrass staggers in sheep grazing ryegrass (Lolium perenne) containing lolitrem B or ryegrass containing both lolitrem B and ergovaline.
METHODS: Ninety lambs, aged approximately 6 months, were grazed on plots of perennial ryegrass infected with either AR98 endophyte (containing lolitrem B), standard endophyte (containing lolitrem B and ergovaline) or no endophyte, for up to 42 days from 2 February 2010. Ten lambs were grazed on three replicate plots per cultivar. Herbage samples were collected for alkaloid analysis and lambs were scored for ryegrass staggers (scores from 0–5) weekly during the study. Any animal which was scored ≥4 was removed from the study.
RESULTS: Concentrations of lolitrem B did not differ between AR98 and standard endophyte-infected pastures during the study period (p=0.26), and ergovaline was present only in standard endophyte pastures. Ryegrass staggers was observed in sheep grazing both the AR98 and standard endophyte plots, with median scores increasing in the third week of the study. Prior to the end of the 42-day grazing period, 22 and 17 animals were removed from the standard endophyte and AR98 plots, respectively, because their staggers scores were ≥4. The cumulative probability of lambs having scores ≥4 did not differ between animals grazing the two pasture types (p=0.41).
CONCLUSIONS AND CLINICAL RELEVANCE: There was no evidence for ergovaline increasing the severity of ryegrass staggers induced by lolitrem B. In situations where the severity of ryegrass staggers appears to be greater than that predicted on the basis of concentrations of lolitrem B, the presence of other tremorgenic alkaloids should be investigated. 相似文献
Precision agriculture is a farming management concept based on observing, measuring and responding to inter- and intra-field variability in crops. In this paper, we focus on responding to intra-field variability in potato crops and analyse variable rate applications (VRAs). We made an overview of potential VRAs in potato crop management in The Netherlands. We identified 13 potential VRAs in potato, ranging from soil tillage to planting to crop care to selective harvest. We ranked them on availability of ‘proof of concept’ and on-farm test results. For five VRAs, we found test results allowing to make a cost-benefit assessment. These five VRAs were as follows: planting, soil herbicide weed control, N side dress, late blight control and haulm killing. They use one of two types of spatial data: soil maps or biomass index maps. Data on costs and savings of the VRAs showed that the investments in VRAs will pay off under practical conditions in The Netherlands. Savings on pesticide use and N-fertilizer use with the VRAs were on average about 25%, which benefits the environment too. We foresee a slow but gradual adoption of VRAs in potato production. More VRAs will become available given ongoing R&D. The perspectives of VRAs in potatoes are discussed. 相似文献