Development and evaluation of a rapid absorbed enzyme immunoassay test for the diagnosis of Johne''s disease in cattle

An absorbed enzyme immunoassay (EIA) test for Johne's disease in cattle was developed in which absorption of cross-reacting antibodies occurred as a rapid reaction in solution rather than overnight with whole organisms and a subsequent centrifugation step. Total test time was reduced to less than 2 h with a minimum of manipulations. The test was evaluated in cattle herds from Johne's disease-endemic and Johne's disease-free regions of Australia. Specificity was 99.8%. Calculations of sensitivity were affected by the history of the herd under test. However, the EIA detected in excess of 80% of animals before onset of clinical disease and 65% of faecal shedders were EIA positive on, or before, first detection of Mycobacterium paratuberculosis in their faeces. The test should aid epidemiological studies and be a useful tool in the management and control of Johne's disease.     

Virus maturation involving large subunit rotations and local refolding

Large-scale conformational changes transform viral precursors into infectious virions. The structure of bacteriophage HK97 capsid, Head-II, was recently solved by crystallography, revealing a catenated cross-linked topology. We have visualized its precursor, Prohead-II, by cryoelectron microscopy and modeled the conformational change by appropriately adapting Head-II. Rigid-body rotations ( approximately 40 degrees) cause switching to an entirely different set of interactions; in addition, two motifs undergo refolding. These changes stabilize the capsid by increasing the surface area buried at interfaces and bringing the cross-link-forming residues, initially approximately 40 angstroms apart, close together. The inner surface of Prohead-II is negatively charged, suggesting that the transition is triggered electrostatically by DNA packaging.     

A High-Temperature Electrical Conduction Mechanism in the Lower Mantle Phase (Mg,Fe)1-xO

Measurements of electrical conductivity at high pressure and temperature were taken on the lower mantle phase magnesiowustite with varying Fe3+ content. Although previous measurements at atmospheric pressure suggest Fe2+-Fe3+ hopping (small polaron) as the dominant conductivity mechanism, the present experiments show a change in charge transport mechanism with temperature. The lower temperature measurements are consistent with small polaron conduction, but at higher temperatures, which are more applicable to the lower mantle, a large polaron mechanism is suggested. Because these mechanisms have different temperature and compositional dependencies, this transition has important implications for extrapolation to mantle conditions.     

Synthesis of Novel Thin-Film Materials by Pulsed Laser Deposition

Pulsed laser deposition (PLD) is a conceptually and experimentally simple yet highly versatile tool for thin-film and multilayer research. Its advantages for the film growth of oxides and other chemically complex materials include stoichiometric transfer, growth from an energetic beam, reactive deposition, and inherent simplicity for the growth of multilayered structures. With the use of PLD, artificially layered materials and metastable phases have been created and their properties varied by control of the layer thicknesses. In situ monitoring techniques have provided information about the role of energetic species in the formation of ultrahard phases and in the doping of semiconductors. Cluster-assembled nanocrystalline and composite films offer opportunities to control and produce new combinations of properties with PLD.     

Global carbon sinks and their variability inferred from atmospheric O2 and delta13C

Recent time-series measurements of atmospheric O2 show that the land biosphere and world oceans annually sequestered 1.4 +/- 0.8 and 2.0 +/- 0.6 gigatons of carbon, respectively, between mid-1991 and mid-1997. The rapid storage of carbon by the land biosphere from 1991 to 1997 contrasts with the 1980s, when the land biosphere was approximately neutral. Comparison with measurements of delta13CO2 implies an isotopic flux of 89 +/- 21 gigatons of carbon per mil per year, in agreement with model- and inventory-based estimates of this flux. Both the delta13C and the O2 data show significant interannual variability in carbon storage over the period of record. The general agreement of the independent estimates from O2 and delta13C is a robust signal of variable carbon uptake by both the land biosphere and the oceans.     

Nitrate leaching losses and their control in a mixed farm system in the Cotswold Hills, England

Abstract. Nitrate leaching was measured for four years at the Royal Agricultural College 's Coates Farm in the Cotswolds, England. Coates is a typical Cotswold mixed farm with thin, well-drained calcareous soils especially prone to leaching. Over the duration of this study there were dairy, sheep and arable enterprises on the farm. A 'Farm Gate' nitrogen (N) budget was constructed. Small 120 m × 20 m 'farmlets' were sited in ten fields across the farm, covering all parts of the rotation, as the sites for detailed measurements. Each farmlet received the same management as the rest of the field in which they were situated. Using ceramic probes inserted to 60 cm, soil water was sampled every two weeks throughout the winter drainage season. The annual drainage varied from 135 mm under grassland in 1996/7 to 600 mm under cereals in 1998/9. Average N losses by leaching were determined mostly by rainfall and were 65 kg N ha^–1 yr^–1, accounting for 25% of the N inputs. Especially leaky parts of the rotation were the ploughing out of a lucerne ley and the grazing of stubble turnips with sheep, both typical Cotswold farm practices. The research highlights some of the difficulties in developing practicable, profitable management practices to decrease nitrate losses.     

Response of organically managed grassland to available phosphorus and potassium in the soil and supplementary fertilization: field trials using grass–clover leys cut for silage

Abstract. Effective use and recycling of manures together with occasional and judicious use of supplementary fertilizing materials forms the basis for management of phosphorus (P) and potassium (K) within organic farming systems. Replicated field trials were established at three sites across the UK to compare the supply of P and K to grass–clover swards cut for silage from a range of fertilizing materials, and to assess the usefulness of routine soil tests for P and K in organic farming systems. None of the fertilizing materials (farmyard manure, rock phosphate, Kali vinasse, volcanic tuff) significantly increased silage yields, nor was P offtake increased. However, farmyard manure and Kali vinasse proved effective sources of K to grass and clover in the short to medium term. Available P (measured as Olsen-P) showed no clear relationship with crop P offtake in these trials. In contrast, available K (measured by ammonium nitrate extraction) proved a useful measurement to predict K availability to crops and support K management decisions.