Producing native seed of Stipa lagascae R. and Sch. inPresaharian Tunisia: high yields, high genetic variation orboth?

Genetic shifts are predicted to be greatest when highly diversematerial originating from an arid environment is bulk multiplied inirrigated and fertilised conditions. This is a key problem if largequantities of quality native seed are needed for restorationpurposes. In Presaharian Tunisia, we investigated two intermingledyield aspects of irrigated seed multiplication of Stipalagascae R. and Sch.: (1) nitrogen fertilisationand (2) selection for vegetative vigour. We set up twocontiguous nitrogen fertilisation trials, one withnon-selected seeds (from a nearby locality), and onewith selected seeds (48 clonal lines seeded in replicatedrows), and compared straw yields. Both selected and unselectedmaterial did not respond to more than 50 kg of nitrogenper ha. Unfertilised yields of both trials were similar,but the straw yield of fertilised selected material was more than100% higher than the straw yield of fertilised unselectedmaterial. In the trial with selected material, we collected straw andleaf yields per row, and scored each row for vigour and for phenologyduring three successive growing seasons. Genotypic differences inseed output and in mortality were large and mainly driven byvariation in potential straw for leaf ratios.The compromise between high yields and high genetic variation couldconsist of the following principles. (1) Create driergrowing conditions so that the actual straw forleaf ratio stays below the genotypic potential. (2) Useinstead fertilisation and selection to enhance seed yields.(3) Avoid bulk multiplication.     

Occurrence of 2n gametes in the F1 hybrids of Oriental × Asiatic lilies (Lilium): Relevance to intergenomic recombination and backcrossing

Cytological modes of the origin of 2n gametes were investigated in six different genotypes of F₁ hybrids between Oriental and Asiatic (OA) lilies (Lilium, 2n = 2x = 24). Chromosome pairing between the parental genomes was very low, the average frequency range from 0.3 to 1.2 bivalents per cell among the genotypes. Within a genotype the frequency of bivalents varied from 0 to 6 in some cases. The normally occurring haploid pollen grains were totally sterile. In contrast, in different genotypes, variable percentages of 2n pollen were found and shown to be fertile as estimated from pollen germination. A cytological analysis of Metaphase I and subsequent stages of meiosis using genomic in situ hybridization (GISH) revealed that there was intergenomic recombination between the alien genomes. Following Metaphase I stage, three different types of abnormal cytological events led to the formation of 2n pollen: (i) Post-Metaphase I division (PMI), (ii) Post-Metaphase II division (PMII) and (iii) Asymmetric Cytokinesis of the pollen mother cell followed by chromosome division. All three cytological events led to first division restitution (FDR) gametes. Based on in vitro pollen germination it was proved for two genotypes that 2n pollen was viable only during the first day of anthesis. It was possible to use 2n pollen successfully for backcrossing. Implications of 2n pollen for intergenomic recombination in BC₁ progenies are discussed.     

Diagnosis of regional evaporation by remote sensing to support irrigation performance assessment

The success of water management in large irrigation schemes with composites of soil, crop, wetness and micro-meteorological conditions is difficult to quantify. Performance assessment indicators, being among others a function of evaporation, are useful tools to evaluate the actual functioning of an irrigation system. The inevitable spatial variability of evaporation in large irrigation schemes makes its determination with conventional point measurements almost impossible. A new remote sensing evaporation parameterization algorithm has been tested with high resolution Landsat Thematic Mapper data for the Eastern Nile Delta, Egypt. Although the implementation of such an algorithm requires assumptions to be made, the current case study shows that these assumptions do not hamper the estimation of actual and potential evaporation at regional scale. The actual evaporation has been used to express the uniformity of crop water use which is related to the equity of irrigation water distribution. The coefficient of variance in actual evaporation between 53 differenent irrigation districts is 10% on average. The relative evaporation was considered to determine whether the crop was adequately irrigated. The relative evaporation was more than 75% for 48 out of 53 irrigation districts. It is concluded, that improved information on actual crop growth conditions through remote sensing provides an essential insight into the planning of real-time and seasonal irrigation water deliveries.     

Genetic association between different criteria to define sexual precocious heifers with growth,carcass, reproductive and feed efficiency indicator traits in Nellore cattle using genomic information

The aim of this study was to estimate genetic parameters for different precocious calving criteria and their relationship with reproductive, growth, carcass and feed efficiency in Nellore cattle using the single‐step genomic BLUP. The reproductive traits used were probability of precocious calving (PPC) at 24 (PPC24), 26 (PPC26), 28 (PPC28) and 30 (PPC30) months of age, stayability (STAY) and scrotal circumference at 455 days of age (SC455). Growth traits such as weights at 240 (W240) and 455 (W455) days of age and adult weight (AW) were used. Rib eye area (REA), subcutaneous fat thickness (SFT), rump fat thickness (RFT) and residual feed intake (RFI) were included in the analyses. The estimation of genetic parameters was performed using a bi‐trait threshold model including genomic information in a single‐step approach. Heritability for PPC traits was moderate to high (0.29–0.56) with highest estimates for PPC24 (0.56) and PPC26 (0.50). Genetic correlation estimates between PPC and STAY weakened as a function of calving age. Correlation with SC455, growth and carcass traits were low (0.25–0.31; ?0.22 to 0.04; ?0.09 to 0.18, respectively), the same occurs with RFI (?0.09 to 0.08), this suggests independence between female sexual precocity and feed efficiency traits. The results of this study encourage the use of PPC traits in Nellore cattle because the selection for such trait would not have a negative impact on reproductive, growth, carcass and feed efficiency indicator traits. Stayability for sexual precocious heifers (PPC24 and PPC26) must be redefined to avoid incorrectly phenotype assignment.     

High throughput phenotyping for aphid resistance in large plant collections

ABSTRACT: BACKGROUND: Phloem-feeding insects are among the most devastating pests worldwide. They not only cause damage by feeding from the phloem, thereby depleting the plant from photo-assimilates, but also by vectoring viruses. Until now, the main way to prevent such problems is the frequent use of insecticides. Applying resistant varieties would be a more environmental friendly and sustainable solution. For this, resistant sources need to be identified first. Up to now there were no methods suitable for high throughput phenotyping of plant germplasm to identify sources of resistance towards phloem-feeding insects. RESULTS: In this paper we present a high throughput screening system to identify plants with an increased resistance against aphids. Its versatility is demonstrated using an Arabidopsis thaliana activation tag mutant line collection. This system consists of the green peach aphid Myzus persicae (Sulzer) and the circulative virus Turnip yellows virus (TuYV). In an initial screening, with one plant representing one mutant line, 13 virus-free mutant lines were identified by ELISA. Using seeds produced from these lines, the putative candidates were re-evaluated and characterized, resulting in nine lines with increased resistance towards the aphid. CONCLUSIONS: This M. persicae-TuYV screening system is an efficient, reliable and quick procedure to identify among thousands of mutated lines those resistant to aphids. In our study, nine mutant lines with increased resistance against the aphid were selected among 5160 mutant lines in just 5 months by one person. The system can be extended to other phloem-feeding insects and circulative viruses to identify insect resistant sources from several collections, including for example genebanks and artificially prepared mutant collections.     

Persistent anthelmintic activity of topically administered ivermectin in red deer (Cervus elaphus L.) against lungworms (Dictyocaulus viviparus)

A controlled trial was conducted to evaluate the persistent anthelmintic effect of ivermectin as a topical treatment at 500 μg/kg against induced infection with lungworm (Dicryocaulus viviparus) in red deer (Cervus elaphus). The results showed a highly significant (p < 0.01) anthelmintic activity for at least 28 days against a newly acquired infection with Dictyocauus viviparus (> 99% efficacy).