Host range of Erwinia psidii and genetic resistance of Eucalyptus and Corymbia species to this pathogen

Dieback caused by Erwinia psidii is currently one of the most important emerging diseases in eucalypt plantations in Brazil. However, little is known in terms of the host range of this pathogen or the potential sources of resistance against the disease it causes. In this study, we inoculated plants of species from nine families to gain insight into the host range of E. psidii. Plants of all inoculated species of Myrtaceae except Acca sellowiana exhibited disease symptoms and therefore represent potential hosts for the pathogen under natural conditions. In addition, the response of four Corymbia species, 29 Eucalyptus species and three interspecific Eucalyptus hybrids to inoculation with E. psidii was evaluated. All Corymbia henryi, Corymbia maculata, Eucalyptus thozetiana, Eucalyptus cloeziana, Eucalyptus viminalis, Eucalyptus dalrympleana and Eucalyptus pilularis plants were highly resistant to the pathogen, whereas differential disease resistance was observed in the other species. This study provides important information on sources of resistance to Erwinia psidii with potential use in the development of clones with enhanced resistance in eucalypt species of economic importance.     

Bidirectional colonization and biofilm formation by Erwinia psidii in eucalypt plants

Dieback and wilt caused by Erwinia psidii is an emerging disease that has been causing considerable damage in eucalypt plantations. Because it is a recently emerged disease, several aspects of the bacterial interaction with its host still remain to be elucidated. In this work, we studied the E. psidii colonization and biofilm formation in eucalypt tissues by specific detection using PCR and scanning electron microscopy (SEM). The results indicate that the bacterium is able to translocate in stem tissue mainly acropetally, although movement in the basipetal direction was also observed to a lesser extent, always through the xylem. No colonization of phloem tissues was observed. In addition to colonizing the xylem, E. psidii colonized the parenchymatous tissue. The bacterium formed cell aggregates enveloped by fibrillar material that evolved into complex, well-structured biofilms in stem and leaf tissues. In contrast, no biofilm formation was observed on abiotic surfaces. These observations suggest that biofilm formation plays an important role in the elicitation of dieback and wilt symptoms caused by E. psidii on eucalypt plants. This study not only shows ultrastructural aspects of the E. psidii communities but also tissue damage in eucalypt plants that was associated with the presence of bacterial aggregates and formation of tyloses.     

Effects of surface‐applied and soil‐incorporated lime on some physical attributes of a Dystrudept soil

It is generally accepted that liming ameliorates soil acidity. However, the method of lime application is thought by many to influence its effectiveness in acid soils. In this study, we wanted to assess the degree of effectiveness of surface‐applied lime and lime incorporated into the soil on soil structural attributes and water retention of a Dystrudept soil in the SE region of the State of Paraná, Brazil. Lime was added at the rate of 15 t/ha to soil through: (i) surface broadcasting, (ii) incorporation via ploughing and harrowing, (iii) incorporation via subsoiling and harrowing. A control treatment with zero lime application was included in the experiment. The addition of lime by surface broadcasting resulted in significant reductions in soil bulk density (BD) and macroporosity (Ma) and increases in total porosity (TP) and microporosity (Mi) of the top soil layer (0–0.10 m). The reverse was the case in the 0.10‐ to 0.20‐m soil layer; where lime was incorporated via ploughing and harrowing, increases in BD and reductions in TP and Ma were observed. Addition of lime also significantly increased soil water retention, with maximum retention recorded from soil amended with surface broadcast lime. Changes in soil chemical attributes (increases in pH, Ca²⁺ and Mg²⁺ contents; reductions in potential acidity and Al³⁺ content) were responsible for the changes observed in structural and physical attributes, and water retention. Bearing in mind the lower application costs, improvement in the soil chemical attributes for plant development and soil physical quality, surface broadcast lime can be considered a promising alternative for no‐till farmers.     

Soil organic matter pools and carbon-protection mechanisms in aggregate classes influenced by surface liming in a no-till system

The stabilisation of soil organic matter (SOM) is the result of the simultaneous action of three mechanisms: chemical stabilisation, biochemical stabilisation and physical protection. The objectives of this study were (i) to evaluate carbon-protection mechanisms in different SOM pools in soil aggregates and (ii) to identify the association of Ca^2 + with total organic carbon (TOC) under the influence of surface liming in a medium-textured Oxisol in a long-term experiment under no-till system (NTS) in southern Brazil (25° 10′ S, 50° 05′ W). The treatments consisted of application of zero or 6 tons ha^? 1 of dolomitic lime on the soil surface in 1993 and a reapplication of zero or 3 tons ha^? 1 of dolomitic lime in 2000 to plots with or without previous lime application. Soil samples collected at depths of 0–2.5, 2.5–5, 5–10 and 10–20 cm were separated into seven aggregate classes. In each of these classes, TOC, particulate organic carbon (POC) and mineral-associated organic carbon (MAOC) were analysed. The 8–19 mm sized aggregates from the 0–2.5 cm layer were assessed by energy-dispersive X-ray spectroscopy (EDS) for the elemental analysis of carbon (C) and calcium (Ca). The liming caused an accumulation of TOC in the aggregates, mainly at a depth of 0–2.5 cm. The aggregates from soils treated with lime had a higher mean weight diameter (MWD) that resulted in the accumulation of TOC, especially in the 8–19 mm aggregate class, that was linear and closely related with C input (R² = 0.99). The proportion of large aggregates in the treatments with lime was closely correlated with the TOC content of the whole sample. The largest dose of lime (9 tons ha^? 1) resulted in higher TOC, POC and MAOC values, mainly in the 8–19 mm aggregate class. The elemental analyses for C and Ca revealed similar spectra between them for the surface-liming treatments in the clay fraction found in the centres of the 8–19 mm aggregates. The surface application of lime to NT fields provided greater stability and protection of the intra-aggregate C, presumably due to Ca^2 + acting as a cationic bridge between OC and the kaolinite in the clay fraction.     

Harvesting an invasive bivalve in a large natural lake: species recovery and impacts on native benthic macroinvertebrate community structure in Lake Tahoe,USA

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Mesquite pod meal as an additive increases milk total solids,lactose, fat and protein content in dairy cows

The objective was to evaluate the intake, digestibility, milk yield and composition of cows fed mesquite pod meal (MPM). Five crossbred dairy cows, with an average body weight of 450?±?5 kg and producing 10?±?1 kg of milk/day were distributed in a 5?×?5 Latin square arrangement with five periods of 20 days each, comprised of 15 days for adaptation to the experimental diets (T1?=?0%, T2?=?1.5%, T3?=?3.0%, T4?=?4.5% and T5?=?6.0% MPM) and 5 days for data collection. Variables were assessed by analysis of variance and regression at P?<?0.01 and P?<?0.05 of significance, using the Student’s test. Animals were kept on pastures and the concentrates were provided at 6 kg/animal divided in two meals per day. The inclusion of 3.0% mesquite pod meal did not affect the intake, digestibility of nutrients and milk yield, but it changed milk composition. The dietary inclusion of 3.0% mesquite pod meal as a palatability enhancer increases the percentage of milk solids, lactose, fat and protein. Therefore, mesquite pod meal can be included in diets for dairy cows to improve the nutritive value of milk.

     

Soil acidity and aluminium toxicity as affected by surface liming and cover oat residues under a no-till system

A 3-year field trial examined in a long-term no-till system the effects of surface-applied lime and cover black oat ( Avena strigosa Schreb) residues on soil chemical attributes, root growth and grain yield of corn ( Zea mays L.) and soybean ( Glycine max L. Merrill) on a loamy, kaolinitic, thermic Typic Hapludox in Paraná State, Brazil. The treatments consisted of dolomitic lime broadcast on the soil surface at 0 or 12 t/ha, with and without cover of black oat residues. Corn and soybeans were grown without rainfall limitation. Applying lime on the surface improved soil acidity and decreased aluminium (Al) toxicity to a 10-cm depth 1 year after application. Surface liming increased pH and the content of exchangeable Ca²⁺ to a 20-cm depth, and decreased Al toxicity to a 40- to 60-cm depth, 3 years after application, indicating that the surface-applied lime moved deeper. Cover black oat residues did not favour the mobility of surface-applied lime to alleviate subsoil acidity and an increase in the Al³⁺ saturation level at the soil surface was found in unlimed plots with black oat residues. Root growth and grain yields of corn and soybean were not influenced by surface liming with or without cover black oat residue. Despite the soil acidity level, root length of corn and soybean ranged from 55 to 60% at 0- to 10-cm depth. The results suggest that Al toxicity is low in no-till systems during cropping seasons with adequate and well-distributed rainfall, but this effect is not related to the presence of cover oat residues.     

Long‐term effects of lime and gypsum additions on no‐till corn and soybean yield and soil chemical properties in southern Brazil

A long‐term experiment on a clayey, kaolinitic, thermic Rhodic Hapludox where dolomitic lime was applied to the surface (either at 4.5 t/ha or at 1.5 t/ha per yr for 3 yr), or incorporated into the topsoil (4.5 t/ha), and gypsum applied to the surface (3, 6, and 9 t/ha), was carried out to evaluate their effects on soil profile chemical properties and yields of corn (Zea mays L.) and soybean (Glycine max L. Merrill). Lime applied to the soil surface at either full or split rates, or incorporated and surface‐applied gypsum had long‐lasting effects on soil acidity or calcium and sulphur availability respectively, as measured 8 yr after application. Grain yields of corn and soybean were not influenced by liming. Gypsum at 9 t/ha significantly increased corn grain yields by 7 and 8% respectively 7–10 yr after application, but did not affect soybean grain yields. The differences in response of the corn and soybean crops to gypsum might be related to the Ca²⁺ uptake by plants because of cation exchange properties of roots, being smaller for corn than for soybean. The use of gypsum in no‐till systems becomes more viable when corn is grown with a greater frequency in crop rotation.