眉县两次气象站迁址对温度资料序列的影响分析

选取经历两次迁址的眉县气象站和周边2个未迁过站址的参照站1974～2017年年平均气温、年平均最高气温、年平均最低气温资料,对比分析了迁址对眉县站温度序列的影响。结果表明:两次迁址和城市热岛效应的共同影响使得眉县站温度变化趋势与参照站产生较大差异。眉县站44年来年平均气温和最高气温升幅比参照站明显,最低气温升幅小于参照站;近44年两站气温差值随时间波动位相基本一致,且与眉县站两次迁址时间刚好对应;年平均气温差值序列有3个不连续点,年平均最高气温差值序列有4个不连续点,年平均最低气温差值序列有5个不连续点,每个时段平均值差异显著;热岛效应和迁址使得眉县站温度年代变化趋势不一致;造成气温差异的主要原因是测站周围环境的变化和下垫面性质的变化。     

今年起城乡居民将免费享受基本公共卫生服务——解读中央财政下拔基本公共卫生服务补助资金104亿元

作为我国深化医药卫生体制改革的一项重要内容,近日,中央财政下拨2009年基本公共卫生服务补助资金104亿元,支持按规定项目免费为全体城乡居民提供基本公共卫生服务.此举标志着我国基本公共卫生服务逐步均等化工作的全面推开.     

A perspective on ovine footrot control

A method for the control of ovine footrot is described which utilises the most appropriate measures to suit the circumstances for individual properties. This approach was used to eradicate the disease from three Central Otago fine-wool properties wintering in excess of 35,000 sheep on widely differing classes of land. This programme incorporates the use of proven.techniques and suitable planning together with input from the rural veterinarian, who plays an important role in demonstration and advising on control measures.     

Blood parasites of sheep in the Netherlands. I. Anaplasma mesaeterum sp.n. (Rickettsiales, Anaplasmataceae)

Summary On two occasions an anaplasm was isolated from sheep on the Dutch island of Ameland. The organism proved to be highly pathogenic for splenectomised sheep; a non-splenectomised animal recovered spontaneously after the packed cell volume had decreased by 40%. Treatment with oxytetracycline was effective. Its pathogenicity for goats appeared to be low, and the organism was apparently not infective to splenectomised cattle. This anaplasm differs from Anaplasma ovis in that less than 30% of the organisms are marginally situated in the red cell, as against over 70% in A. ovis; cross-immunity with A. ovis was incomplete and the latter appeared to be far more pathogenic to goats than the Dutch anaplasm, for which the name Anaplasma mesaeterum sp.n. is proposed. Its ultrastructure is similar to that of A. marginale and A. ovis. The vector is either Ixodes ricinus or Haemaphysalis punctata. Its practical importance remains to be ascertained.     

H3亚型猪流感病毒荧光定量PCR检测方法的建立

通过RT-PCR方法克隆了H3亚型猪流感病毒HA基因一段靶序列,构建重组质粒作为标准阳性模板.根据GenBank中的H3亚型猪流感病毒HA基因保守序列设计了用于FQ-PCR的1对引物和1条TaqMan探针.通过条件优化,以10倍系列稀释的质粒为标准品进行荧光定量PCR扩增,并制作标准曲线,建立了检测H3亚型猪流感的荧光定量PCR方法.结果表明,该方法检测灵敏度可达1.0×100拷贝/μL,线性范围为109～100,达10个数量级;对起始浓度为1.0×109、1.0×108、1.0×107拷贝/μL的标准品的最终实际测得值(Ct)分别为13.68,18.21和20.57;变异系数分别为0.31%、0.17%和0.12%,均小于5%,说明此方法具有良好的准确性和重现性.对阳性组织病料的检测表明,该方法的检测灵敏度高出常规PCR,与套式PCR具有相近的灵敏度.