Commercial cultivation of Bt cotton produced higher boll load which led to stiff inter-original competition for photosynthates, resulting in early cessation of growth (premature senescence) due to more availability of sink and less sources. To overcome this problem, field experiment was conducted during 2011 and 2012 using five treatments of plant growth manipulation viz. no fruiting branch removal (F1), removal of first fruiting branch (F2), removal of first and second fruiting branch (F3), removal of all squares from first fruiting branch (F4), removal of all squares from first and second fruiting branches (F5), and three potassium (K) application rates viz. 50 kg ha?1 (K1), 100 kg ha?1 (K2), and 150 kg ha?1 (K3). More nodes above white flower were recorded in F5, followed by F3, while minimum were recorded in F1. Among potassium levels, maximum nodes above white flower were recorded in K3 followed by K2 and K1 during both years of study. Plant height recorded at physiological cutout stage or at maturity stage showed that plants gained more height with removal of all squares from first tosecond fruiting branches with higher potassium dose. Leaf K increased with increasing applied potassium and also with square/branch removal. So early removal of squares/fruiting branches along with higher potassium dose helped in delaying canopy senescence in Bt cotton. 相似文献
Peach fruit is enriched with natural antioxidants but oxidation caused by biotic and abiotic stresses, reduce these antioxidants and consequently effect the quality and yield of the fruit. Therefore, an experiment was conducted to investigate the role of ascorbic acid to sustain the natural antioxidant activity, improve the fruit quality and yield of peach cultivars (Early Grand and Florida King). The peach cultivars, Early Grand and Florida King were tested against four levels of ascorbic acid (200, 400, 600 and 800?ppm) and compared with control as water spray. The foliar application of ascorbic acid significantly influenced anti-oxidant activity and physico-chemical attributes of peach cultivars. However, the foliar application of ascorbic acid @ 800?ppm significantly increased the fruit weight, fruit yield, fruit firmness, total soluble solids, titratable acidity, antioxidant activity of fruit peel, with least percent infected fruits and fruit juice pH as compared to the rest of the treatments. Similarly, significant variation was recorded for peach cultivars regarding anti-oxidant activity and various physico-chemical attributes. The maximum fruit weight, fruit yield, fruit firmness, total soluble solids, titratable acidity and TSS/TA ratio were noted in fruit of cultivar Florida King. On other side the foliar treated fruit of peach cultivar Early Grand had the highest percent infected fruits, fruit juice pH and antioxidant activity of fruit peel. It is concluded from the significant findings of present research that the peach cultivar Florida King performed better in terms of maximum fruit yield, whereas the cultivar Early Grand produced better quality fruits with high anti-oxidant activity when treated with 800?ppm of ascorbic acid as foliar spray. 相似文献
Cold arid trans-Himalayan Ladakh region of India bordering with Pakistan and China is one of most elevated inhabited regions of the world. This region is highly vulnerable to climate change and serious environmental threats resulting in reduction in crop productivity due to risk of agricultural pest and plant pathogens. The present work describes the various diseases attacking barley from this region where the status of barley diseases has not yet been explored. Extensive field surveys were conducted for the first time during 2004-2005 and 475 fields were inspected at the 95 locations. Yellow rust, powdery mildew, leaf spot blotch/blight, covered smut, loose smut, foot/root rot and cereal cyst nematode causing molya disease were encountered during the survey. However, yellow rust, molya and foot/root rot were found as the most destructive diseases. Excellent hotspots of the encountered diseases were identified and marked which may be used for the screening of barley genotypes and determining the impact of climate change on plant diseases. 相似文献
Reasons for performing study: Mesenchymal stromal cells (MSCs) represent an attractive source for regenerative medicine. However, prior to their application, fundamental questions regarding molecular characterisation, growth and differentiation of MSCs must be resolved. Objectives: To compare and better understand the behaviour of equine MSCs obtained from bone marrow (BM) and adipose tissue (AT) in culture. Methods: Five horses were included in this study. Proliferation rate was measured using MTT assay and cell viability; apoptosis, necrosis and late apoptosis and necrosis were evaluated by flow cytometry. The mRNA expression levels of 7 surface marker genes were quantified using RT‐qPCR and CD90 was also analysed by flow cytometry. Differentiation was evaluated using specific staining, measurement of alkaline phosphatase activity and analysis of the mRNA expression. Results: High interindividual differences were observed in proliferation in both cell types, particularly during the final days. Statistically significant differences in viability and early apoptosis of cultured AT‐ and BM‐MSCs were found. The highest values of early apoptosis were observed during the first days of culture, while the highest percentage of necrosis and late apoptosis and lowest viability was observed in the last days. Surface marker expression pattern observed is in accordance to other studies in horse and other species. Osteogenic differentiation was evident after 7 days, with an increasing of ALP activity and mRNA expression of osteogenic markers. Adipogenic differentiation was achieved in BM‐MSCs from 2 donors with one of the 16 media tested. Chondrogenic differentiation was also observed. Conclusions: Proliferation ability is different in AT‐MSCs and BM‐MSCs. Differences in viability and early apoptosis were observed between both sources and CD34 was only found in AT‐MSCs. Differences in their osteogenic and adipogenic potential were detected by staining and quantification of specific tissue markers. Potential relevance: To provide data to better understand AT‐MSCs and BM‐MSCs behaviour in vitro. 相似文献
Removal of early fruiting branches with greater potassium doses caused more source and no sink at early stages of growth, leading to improved yield, yield components, and fiber quality traits in Bt cotton. The study used manual alteration of plant architecture (F1, no branch removal; F2, removal of first fruiting branch; F3, removal of first and second fruiting branches; F4, removal of all squares from first fruiting branch; F5, removal of all squares from first and second fruiting branches) and potassium rates (50, 100, and 150 kg ha?1) in a randomized complete block design and was repeated for 2 years (2011 and 2012). Increasing potassium application increased total bolls per plant and cotton yield to the greatest levels in F3 and F5, against lowest level in the control. Ginning out turn, fiber length, seed oil, and seed protein content were influenced by fruiting branch or square removal but the difference was less. Increasing potassium improved seed and fiber quality. 相似文献
Rapid industrialization in China accelerated environmental pollution by xenophores and trace metals particularly cadmium. Numerous studies have been conducted to address soil contamination using organic and inorganic amendments under pot or incubation conditions, but few were performed under field conditions, particularly in the wheat-growing areas. This study aims to investigate the effectiveness of pre-applied soil amendments for Cd immobilization, changes in soil pH, and metal uptake by wheat.
Materials and methods
In this study, 12 treatments, i.e., control (CK), lime, DaSan Yuan (DASY), DiKang No. 1 (DEK1), biochar, Fe-biochar, Yirang, CaMgP, and green stabilizing agents (GSA-1, GSA-2, GSA-3, and GSA-4), were evaluated for Cd immobilization in incubation and field experiments. Changes in soil pH and available metal content with amendments were monitored from 30 to 150 days of incubation. Single extraction method and DTPA-extractable and sequential extraction procedures were employed to assess the variations in available Cd contents of field soil. The impact of soil amendments on Cd uptake and bioaccumulation in food part of wheat was measured at harvesting stage in a contaminated site.
Results and discussion
Lime treatment increased soil pH by 32% and reduced available Cd to 0.213 mg kg?1, as compared to CK (control) in the incubation experiment (0.343 mg kg?1). Effects of the amendments on wheat growth, soil pH, and Cd phytoavailability were reported. Wheat biomass was highest in treatment GSA-2 (13,880 kg ha?1) and GAS-4 resulted in an increase in grain yield (5350 kg ha?1). Soil pH of the treated field at harvesting stage increased up to 6.50, 6.50, and 6.47 by application of GSA-4, GSA-2, and lime, respectively. Cadmium contents in wheat grain were declined significantly in the treatments of GSA-2, GSA-4, and lime.
Conclusions
Results of this study revealed a significant decrease in available Cd concentration with the application of amendments. Composite amendments were more effective in terms of metal uptake and bioaccumulation in wheat. In addition, our results indicate the effectiveness of composite amendments in stabilizing Cd in contaminated soil.
Effects of fodder availability and prolificacy on birth weight, weaning weight, weight at 6 and 9 months age, and pre- and
post-weaning growth rates of Dera Din Panah goat were determined. A total of 350 records of Dera Din Panah (DDP) goats maintained
at Sheep and Goat Development Centre, Rakh Khairewala, District Layyah, Punjab, Pakistan were analyzed using Harvey’s Mixed
Model Least Squares and Maximum Likelihood computer program. Fodder availability significantly affected (P < 0.05) the birth
weight, weaning weight, and pre-weaning daily gain. The effect of prolificacy on the birth weight, weaning weight, pre-weaning
growth rate was significant (P < 0.05). Effects of fodder availability and prolificacy on average daily weight gain during
different stages, such as from birth to 3 months of age (GR1), 3 to 6 months of age (GR2), 6 to 9 months of age (GR3), 9 to
12 months of age (GR4), and overall growth rate from birth to 12 months of age (GR5) age were determined. 相似文献
The aim of this study was to investigate the effect of various calcium:total phosphorus (Ca:tP) ratios due to addition of microbial phytase and 1,25-dihydroxycholecalciferol [1,25-(OH)2D3] on the absorption levels of minerals. In a 42-day experiment repeated three times, 144 day-old male broiler chicks (ISA 220) were divided into six groups of eight chicks each. Diets containing two different (1:1 and 2:1) Ca levels were prepared. Groups 1, 2 and 3 received a diet of 1:1 Ca:tP ratio while Groups 4, 5 and 6 a diet of 1:2 Ca:tP ratio. These diets contained 1,25-(OH)2D3 and phytase in levels of 5 micrograms/kg and 600 FYT/kg, respectively. The faeces was collected to analyse the absorption of minerals. At the end of the study, the absorption levels of Ca, P, Zn, Mn and Cu were increased by the addition of phytase enzyme (p < 0.05). This effect was obvious in the 3rd week. On the other hand, in the 6th week only Ca and P absorption levels were influenced positively by the addition of phytase enzyme. The results proved the positive effect of phytase, an enzyme which is used for increasing the utilisation rate of phytate P, Ca and other minerals in broilers. 相似文献