Production of Lambs After the Transfer of Fresh and Cryopreserved in vitro Produced Embryos from Prepubertal Lamb Oocytes and Unsorted and Sex-sorted Frozen-thawed Spermatozoa

Cumulus-oocyte complexes from hormone-stimulated 3-4-week-old (n=43) and 6-7-week-old (n=12) prepubertal lambs were matured in vitro and incubated with unsorted, or X- or Y-spermatozoa separated with a high-speed cell sorter (SX MoFlo)frozen-thawed. Presumptive zygotes were then cultured to the blastocyst stage, and transferred to recipients fresh or after cryopreservation (frozen). Oocyte cleavage was higher (p <0.05) with unsorted (515/926, 55.6%) than X- or Y-spermatozoa (261/672, 38.8% and 229/651, 35.2%, respectively) and blastocyst formation (% zygotes) by Day 9 of in vitro culture was lower (p <0.05) for X- (102/261, 39.1%) than unsorted spermatozoa (249/515, 48.3%), but did not differ between Y-spermatozoa (103/229, 45.0%) and unsorted spermatozoa, or between X- and Y-spermatozoa (p >0.05). For fresh embryos, survival to term was 50.0% (3/6) for unsorted, 0.0% (0/6) for X- and 16.7% (1/6) for Y-spermatozoa-derived embryos (p >0.05), and for frozen embryos was 4.0% (2/50) for unsorted, 9.1% (2/22) for X- and 2.9% (1/34) Y-spermatozoa-derived embryos (p >0.05). Of the two lambs born from X-spermatozoa-derived embryos, one was female (50%), and from the two Y-spermatozoa-derived lambs, both were male (100%), demonstrating that lambs can be produced after the transfer of fresh and cryopreserved IVP embryos derived from prepubertal lamb oocytes and frozen-thawed sex-sorted sperm.     

Biology of the tench, Tinca tinca (L.), in an Irish lake

Abstract. During the summer of 1986 a fyke-netting programme in Lough Leane, Killarney, County Kerry, for tench, Tinca tinca (L.), yielded 142 tench of which 107 were retained for analysis. Age, growth, feeding and fecundity studies were carried out on these fish.
The trophic status of Ross Bay in Lough Leane, which is known to have been eutrophic since 1971, is thought to be responsible for the improved growth of the tench when compared with two other Irish lakes, Coosan Lough, County Westmeath and College Lake, County Cork.
The meristic data recorded for Lough Leane tench show variations from those listed by Lelek (1987) for tench from other European waters. These variations may be related to the low water temperatures which curtail tench spawning in Ireland, particularly in Lough Leane.     

A seasonal analysis of the diet and feeding dynamics of brown trout, Salmo trutta L., in a small nursery stream

Abstract. The diet of brown trout, Salmo trutta L., in the Owendoher Stream, a tributary of the River Dodder, was examined at monthly intervals from June 1981 to May 1982. The diets of the three main age groups, 0+, 1 + and 2+/3+ fish, have been separately described and compared. Ephemeropteran nymphs, larval and adult chironomids as well as a variety of other adult insects were the most frequently consumed foods. While 0+ trout largely concentrated on aquatic organisms, adult insects, in particular those of terrestrial origin, became progressively more important in the diet with increasing age of the fish. Daily ration calculations indicated that food intake was highest during the summer and early autumn months when it was well in excess of maintenance requirements. This corresponded to a period of good growth. For the remainder of the year food intake was not much higher than maintenance requirements. In fact, 1+ and older (2+/3+) fish probably experienced an energy deficit on occasions during the winter which was reflected in their poor condition and weight loss. The relationship between growth and production in the system are further discussed.     

百菌清在番茄上的自然消解动态及健康风险研究

试验设喷施百菌清推荐剂量(2 400g/hm2,T1)、加倍剂量(4 800g/hm2,T2)、喷清水(对照)3个处理。探讨蔬菜上百菌清残留量的安全性,开展番茄中百菌清残留的消解规律试验。结果表明,百菌清在番茄上的自然消解速度随施药剂量的增大而减慢。喷药后24h内消解最快,T1处理和T2处理施药后1d的消解率分别为60.22%和27.58%,7d的消解率达91.72%和46.67%,其半衰期分别为4.3d和18.0d。按推荐剂量施药1d后采摘,番茄中百菌清残留量为0.370mg/kg,符合国家相关标准的规定;从人体健康风险角度分析,如果按规范施药,百菌清在番茄上施用安全,产品食用无健康风险。     

Brown trout, Salmo trutta L., production in an Irish coastal stream

Abstract. Brown trout. Salmo trutta L., estimates were obtained for the Owendoher, a tributary of the River Dodder. Data were collected at live stations on 21 occasions from May 1981 to May 1983. Total population densities ranged from 0·07 to 1·29 fish/m² Mean annual densities varied from 0·20 to 0·77 fish/m². Trout in this stream are typically slow-growing fish reaching a maximum length of 7·68, 13·05 and 17·54cm at the end of their first, second and third years respectively. Growth improved in a downstream direction with the highest rates recorded in stretches overlying limestone. There was some evidence of density-dependent growth in the upper reaches Daily instantaneous growth rates were highest for all age groups during the months May to August but became depressed during the winter. There was considerable variation in the total monthly biomass figures with values ranging from 1·32 to 33·46 g/m². Mean annual values ranged from 3·96 to 13·48 g/m². Annual production (somatic) varied from 3·77 to 14·38 g/m². in 1981–1982 and from 6·12 to 12·70 g/m². in 1982–1983. The mean annual production figure for this stream was 11·24 g/m². Production was contributed mainly by 1+ and 2+ trout at all stations. Egg production. accounting for 3–4% of the total estimate, was contributed mainly by resident 1+ and 2+ fish. It ranged from 0·33 to 0·45 g/m² in 1981–1982 and from 0·14 to 0·53 g/m² in the following year. Ratios of annual production to mean biomass (P/B?) were highest for 0+ trout hut decreased with increasing age. The mean P/B? ratios for all age classes varied from 0·95 to 1·17. The mean value for the Owendoher stream was 1·1. The effects on production of changes in age structure, population density and habitat alterations are discussed.