急性感染肝片吸虫水牛血细胞免疫反应的研究

 研究了水牛急性感染肝片吸虫后血细胞免疫反应在抗感染中的作用机制：结果表明．水牛感染后．ＷＢＣ的总体水平在整个感染期高于对照组；ＤＣ中的嗜酸性粒细胞（％）在第 ２周即显著高于对照组直到 １９周．以后下降并接近对照组；嗜中性粒细胞（％）在第５～１６周低于或明显低于对照组．以后两组接近；淋巴细胞（％）在感染后的第２～５周低于或明显低于对照组．以后接近．至第１４周又低于或明显低于对照组直至２１周；单核细胞和嗜碱性粒细胞（％）在整个实验期无显著差异；Ｔ－淋巴细胞（％）从第１周起显著下降，Ｂ－淋巴细胞（％）显著升高．１１周后均与对照组无明显差异；ＲＢＣ－ＣＲ１花环率在２～１６周显著低于对照组．以后逐渐恢复接近对照组，而ＲＢＣ－ＩＣ花环率在２～１８周高于或明显高于对照组。提示水牛在急性感染肝片吸虫后，血细胞的特异性和非特异性免疫反应变化剧烈，共同参与抗肝片吸虫感染机制。     

急性感染肝片吸虫水牛血中几种花生四烯酸代谢物及激素动态变化

选择12头健康、2～3岁、体重300～400kg雄性去势水牛，粪便检查和Dot—ELISA检测确认无肝片吸虫感染，随机分成感染组(n=9)和对照组(n=3)。感染组每头经口一次性感染1600个肝片吸虫囊蚴。研究了水牛急性感染肝片吸虫后血中几种花生四烯酸代谢物及激素动态变化。结果表明：水牛在急性感染肝片吸虫后整个实验期里没有明显的临床症状，具有较大的耐受力和抵抗力；水牛感染后，PGE2在16周前高于或明显高于对照组，以后两组相近；6—keto—PGF1α在整个实验期感染组与对照组无明显差异；TXB2在第1—10周低于或明显低于对照组。血浆Cort在第4—10周高于或明显高于对照组；T4在第4—22周总体水平高于或显著高于对照组；Ins和T3在整个实验期两组比较未见明显差异性变化。结果提示：水牛在急性感染肝片吸虫后，血中一些花生四烯酸代谢物变化明显，参与病理生理发生发展过程。     

Study on Blood Cell Immune Response in Water Buffaloes Infected Acutely with F. hepatica

Action mechanism of blood cell immune response in water buffaloes against acute infection with F. Hepatica was studied. The results showed that after water buffaloes were infected, the total levels of WBC surpassed control group during whole infection period; Eosinophiles (%) of DC were higher than control group at the 2nd week until 19th week, and then dropped and was close to control group; Neutrophiles(%)was low or significantly lower than control group within the 5 - 16th weeks; The total levels of lymphocytes (%) was lower than control group during the whole infection period; T-lymphocytes (%) dropped significantly, but B-lymphocytes(%) had opposite changes from the first week of infection, and they were close to the control group after 11 weeks; RBC-CR1 and RBC-IC rosette rates dropped and rose during 2 - 16 and 2- 18 weeks, respectively, and then approached the same between both groups. It was suggested that the violent changes of specific and nonspecific immune responses in water buffaloes with acute F. hepatica infection are related with the mechanism against infection with F. hepatica together.