In many human cancers, the expression of the prostaglandin receptor EP4 (EP4R) is associated with the development of malignancy and a poor prognosis. The expression of EP4R has not yet been evaluated in canine tumors. The objective of this study was to characterize the messenger RNA (mRNA) expression of EP4R in canine osteosarcoma (OSA). Gene expression of EP4R was evaluated using RNA in-situ hybridization (RNAscope). In all canine OSA samples evaluated, strong universal positive expression of EP4R was identified. Gene expression was significantly higher in OSA tissue samples than in normal nasal turbinate bone, possibly implicating EP4R in the pathogenesis of canine OSA. 相似文献
Dilution rates for pooled faecal culture (PFC) and direct IS900 polymerase chain reaction (D-PCR) tests were evaluated on faecal samples from infected cows mixed with uninfected faeces in dilutions from 1 in 5 to 1 in 50. PFC was performed by radiometric culture, with confirmation by IS900 PCR and restriction endonuclease analysis (PCR/REA) on growth, and by mycobactin dependency testing on solid medium. Using 37 culture positive faecal samples from 12 subclinical cows, 83.8% and 94.6% of samples were confirmed positive in the PFC assay at dilutions of 1 in 50 and 1 in 30, respectively. Lower dilutions (1 in 5 to 1 in 20) provided only marginally better sensitivity, and confirmation of PFC growth by PCR/REA was significantly more sensitive than mycobactin dependency. D-PCR had significantly lower sensitivity than PFC confirmed by PCR/REA, with pools of 1 in 50, 30, 10 and 5 yielding positive results in 64.9%, 70.3%, 78.4% and 83.8% of samples, respectively. Cattle considered to be shedding 1.5 x 10(6) viable M. avium subsp. paratuberculosis (Map)/g faeces (on the basis of estimated losses in processing and growth rates in radiometric broth) were positive at dilutions up to 1 in 50 in the PFC and D-PCR. Those shedding 5 x 10(5) viable Map/g were positive in the PFC at dilutions up to 1 in 40, but required a 1 in 10 dilution or less for D-PCR. The results suggest that for cattle shedding relatively high concentrations of Map in faeces (>2 x 10(5) viable Map/g), maximal dilutions of 1 in 30 for PFC and 1 in 10 for D-PCR would be applicable. 相似文献
We examine the relationship between the number of bird species and environment within 500 000 ha of eucalypt forests in south-eastern New South Wales, Australia. Birds were surveyed at 39 sites within 31 eucalypt communities, which were, in turn, scored by altitude, temperature, rainfall, basal areas of trees and levels of the nutrients nitrogen, phosphorus, potassium and magnesium in the eucalypt foliage.
Ninety bird species were recorded. Numbers per site, averaged over the whole sample period, ranged between 20 and 38 over all surveys, 24 and 42 in ‘summer’ (October to March) when migrant species were present, and 13 and 38 in ‘winter’ (April to September). A forward stepwise Poisson regression model was used to fit bird species richness to the environmental variables. Over all surveys and in ‘summer’, foliar magnesium and tree basal area (including the basal area of dead trees) were significantly (positive) correlated with the number of bird species. In ‘winter’ the correlations were with altitude and temperature (both negative), presumably the result of emigration of species that avoid cold weather, and tree basal area (positive).
The positive association of number of species over all surveys and in ‘summer’ with tree basal area, including dead trees, and foliar magnesium may index the level of forest maturity and productivity.
Forest management directed solely to timber production reduces the basal area and the number of dead trees and may thereby reduce bird species richness permanently.
A methodology is offered, based on the relationship between the physical environment and the more productive eucalypt communities, for determining forest areas containing the most species of birds. 相似文献
Amounts of DDT and its breakdown products were determined in soil in an apple orchard in Herefordshire. Samples were taken for a number of years (1972–79) after use of the insecticide in the orchard had ceased in 1969. The results were compared with those obtained in an investigation of the same orchard in 1968. From 1968 to 1979, soil residues of pp′-DDT, p′--DDT and pp′--TDE decreased gradually whereas those of pp′--DDE increased, and there were linear relationships between log (concentration) and time. The calculated time for 50% decrease in concentration (Dt50) was 11.7 years for pp′--DDT, 3.3 years for pp′--TDE and 7.1 years for op′--DDT; the time for doubling the concentration for pp′--DDE was 9.1 years. Regression analysis on the two major components (pp′--DDT+pp′--DDE) indicated that the total amount (2.7 mg kg?1) was not decreasing with time. It was concluded that during a post-spray era, the breakdown of pp′--DDT to pp′--DDE was a significant feature of the persistence of DDT, and that, in contrast to the findings of other workers who sampled when DDT was being used, there were no losses by volatilisation. There was an exponential decrease in the amount of DDT residues with increasing soil depth and approximately 90% was found in the top 10 cm of the undisturbed soil profile. 相似文献
Benzimidazole and its 2-amino analogue were non-toxic to Lumbricus terrestris when administered orally. The fungicidal 2-substituted benzimidazoles (benomyl, carben-dazim, fuberidazole and thiabendazole) and l-(benzimidazol-2-yl)-3-butylurea were highly and equally toxic. The equitoxicity of compounds with widely differing substituent groups suggests a common mode of action—the benzimidazole moiety being the active part of the molecule. It is likely that there is a common mode of action for benzimidazole analogues for both fungicidal activity and earthworm toxicity. There was no detectable effect on the cholinesterase levels of earthworms poisoned by benomyl. But it is confirmed that the cholinesterase inhibitory activity of benomyl in vitro is due to formation of butyl isocyanate. 相似文献
The final stages of ovarian follicle growth in cattle are typically characterized by the ultrasound-detectable emergence of a cohort of small (3-5mm in diameter) antral follicles, followed by a selection process during which the number of follicles continuing to grow decreases. Finally, only one follicle (the dominant follicle; DF) shows an enhanced growth rate and estradiol synthesis when it attains 8.5mm compared to its closest competitor (the largest subordinate follicle; SF). Cohort emergence is caused by a transient FSH rise, while DF selection occurs during declining FSH indicating differential FSH dependence of DF and SF. In order to elucidate the mechanisms underlying DF survival or SF atresia, this review aims to (i) describe follicular changes in the local production and regulation of members of the inhibin family of proteins and the insulin-like growth factor (IGF) system in relation to FSH deprivation leading to DF selection, and (ii) develop a model for DF selection outlining the putative involvement of inhibins, activin and follistatin on the one hand, and bioavailable IGFs regulated by IGF binding proteins (IGFBPs) and IGFBP proteases on the other hand. It is concluded, that the first indications of differential FSH dependence are seen within 33h of the FSH peak, and high amounts of precursor forms of inhibin and free activin, and low amounts of the lower molecular weight (MW) IGFBPs are related to follicle survival in terms of enhanced growth and estradiol synthesis, and suppression of granulosa cell apoptosis. In addition, maintenance of low amounts of intrafollicular IGFBP4 may constitute an important mechanism in the future DF to attain FSH independence. 相似文献