Effect of Daily Food Supplementation with Essential Fatty Acids on Canine Semen Quality

Polyunsaturated fatty acids are important membrane components that influence membrane integrity and fluidity. In the present study, the effect of oral supplementation for 60 days with essential fatty acids (omega 3, 6 and 9) and vitamin E on canine semen quality was evaluated. Sixteen dogs were selected for the experiment; eight were used as the control group and eight received the fatty acid supplemented diet for 60 days. Semen samples were taken every 15 days during the entire experimental period and were analyzed for volume (ml), motility (%), vigour (0–5), concentration (×10⁶/ml), morphology of spermatozoa (%), plasma membrane integrity (%; using the hyposmotic swelling test) and thermoresistance (motility and vigour after 4 h at 38°C). We concluded that, daily supplementation with omega 3, omega 6 and omega 9 fatty acids, together with vitamin E, for a period of 60 days, significantly increased the semen volume of the treated group after 15 days of supplementation; the vigour and concentration of spermatozoa were superior after the first month of supplementation, while the percentage of morphologically abnormal spermatozoa decreased and the cells were protected against thermal stress.     

Evaluation of a citrate-based anticoagulant with platelet inhibitory activity for feline blood cell Counts

Abstract: Aggregation of feline platelets in vitro results in difficulty assessing platelet number. A citrate-based anticoagulant containing the platelet inhibitors theophylline, adenosine, and dipyridamole (CTAD; Diatube-H, Becton Dickinson, Oxford, UK) has been developed for use in human platelet studies and heparin assays. To evaluate the efficacy of CTAD in reducing platelet aggregation in feline blood samples, aliquots of blood from 51 cats were anticoagulated with EDTA, CTAD, and for 12 samples, citrate solution. Samples preserved in CTAD had significantly higher (P ≤ .001) platelet counts, as determined by an impedance counter, hemacy-tometer, and smear estimation, than samples preserved in EDTA. In addition, subjective assessment of blood smears showed significantly fewer platelet aggregates (P<.001) in CTAD-treated samples compared with EDTA samples. Although values were similar, automated platelet counts and smear estimates of platelet number were significantly higher (P < .05) and platelet aggregation was significantly less (P < .05) in CTAD samples than in citrate samples. These results suggest that the platelet inhibitory activity of CTAD reduced feline platelet aggregation. Automated total WBC counts in CTAD samples were significantly lower (P<.001) than automated counts in EDTA samples but were similar to manual WBC counts in EDTA samples. Differences in both platelet and WBC counts between CTAD and EDTA or citrate samples were clinically relevant. Mean platelet volume and MCV were significantly lower (P< .05) in CTAD samples than in EDTA samples. No effect was seen on cell morphology or staining characteristics. The anticoagulant CTAD offers an advantage over both EDTA and citrate for feline hematologic analysis, by decreasing pseudothrombocytopenia and pseudoleukocytosis.     

The characterization of cycad palm toxicosis and treatment effects in 130 dogs

The purpose of this retrospective study is to characterize the clinical signs, laboratory values, treatment modalities and mortality outcomes related to cycad palm toxicosis in dogs and to identify medical interventions that potentially correlate with mortality. Dogs with confirmed cycad palm ingestion were identified by reviewing medical records from two private veterinary referral centres for key terms and phrases indicative of cycad palm toxicosis. Of 130 dogs included, 107 (82.3%) presented with clinical signs. A total of 2 (1.5%) died and 14 (10.8%) were euthanized. Diarrhea, lethargy and mortality were all associated with an elevated initial alanine transaminase (ALT) concentration. Overall mortality was 12.3%, with a significantly higher proportion of dogs with an ALT concentration <125 U/L surviving compared to dogs with an elevated ALT concentration. Treatment with activated charcoal reduced the odds of death among all dogs by 82% and was even more protective among dogs with an elevated initial ALT concentration. Among dogs that had an initial platelet count performed, the presence of thrombocytopenia (<200,000/μL) was a negative prognostic indicator and was associated with a significant increase in mortality. Given the high mortality rates reported for cycad palm toxicosis, it is imperative to identify early clinical indicators of mortality as well as treatments that reduce mortality.     

Modulation of Ovarian Function in Female Dogs Immunized with Bovine Luteinizing Hormone Receptor

Adult female dogs were immunized with 0.5 mg bovine luteinizing hormone receptor (LH-R) encapsulated in a silastic subdermal implant and subsequently with four intramuscular booster injections of 0.1 mg LH-R each. Circulating LH-R antibody was detected in the sera 3 weeks post-implant. The appearance of LH-R antibody was associated with a decline in the serum progesterone concentrations to a range of 0–0.5 ng/ml until day 365 in the immunized dogs in comparison with a range of 5–10 ng in the control animals, suggesting a lack of ovulation and corpus luteum function in immunized dogs. The immunized dogs did not show signs of `standing heat' and failed to ovulate when induced by LH-RH challenge. Serum oestradiol levels, however, remained in the range of 30–40 pg/ml in both the immunized and the control dogs. With the decline in the antibody titres, the hormonal profile and vaginal cytology returned to a fertile state and the dogs exhibited signs of `standing heat', as well as vaginal bleeding. Dogs immunized with LH-R did not show any serious metabolic, local or systemic adverse effects. The hypothalamic–pituitary gonadal axis remained intact as indicated by little difference in pituitary LH levels between control and immunized animals, and by the release of LH by LH-RH challenge. These studies demonstrate that active immunization of female dogs with LH-R could immunomodulate ovarian function to cause a reversible state of infertility. It may be postulated that, due to extensive interspecies homology, a recombinant LH receptor-based immunocontraceptive vaccine may also be effective in other vertebrates.     

Immunolocalization of Melatonin and Follicle‐Stimulating Hormone Receptors in Caprine Ovaries and their Effects During in vitro Development of Isolated Pre‐Antral Follicles

The expression of melatonin type 1 (MT1) and FSH (FSHR) receptors in caprine ovaries and the effects of these hormones on the in vitro development of isolated pre‐antral follicles were evaluated. Follicles (≤200 μm) were cultured for 12 days in α‐MEM (control) or melatonin (100 or 1000 pg/ml) or sequential melatonin medium (100 pg/ml: from day 0 to day 6; 1000 pg/ml: from day 6 to day 12; experiment 1) and in control or sequential FSH (100 ng/ml from day 0 to day 6; 500 ng/ml from day 6 to day 12) or sequential melatonin or this latter plus sequential FSH (experiment 2). MT1 and FSHR expressions were observed in granulosa cells from secondary and antral follicles. The oocytes from primordial and primary follicles also express FSHR. Sequential melatonin increased the percentage of normal follicles and oocyte recovery compared with the control or melatonin (1000 pg/ml) at day 12. In experiment 2, all the treatments increased the normal follicles and growth compared with the control. In conclusion, this study demonstrated the presence of MT1 and FSHR in caprine ovaries. The addition of increased concentrations of melatonin (sequential medium) or FSH can be used to promote the in vitro development of caprine pre‐antral follicles.     

Use of multivariate techniques to identify vegetation units and monitor change on a livestock production system in a semi‐arid savanna of the eastern Cape

Species composition data of the grass layer were determined from point surveys along permanently demarcated transects. These data were separated into vegetation units using multivariate techniques. The repeatability of this classification was tested on data collected over a period of time. The results indicate that classification based on composition data collected from a single sampling period was adequate for practical purposes provided certain precautions were taken. Change in vegetation was assessed by ordering sites from repeated measurements and tracing site trajectories through ordination space. The trends along the axes were strongly correlated with assessed condition score.     

Effect of continuous grazing in the dohne sourveld on species composition and basal cover

Abstract

In a grazing trial conducted at the Dohne Agricultural Research Station, natural grassland was grazed continuously for 36 years with cattle. In a second investigation conducted on similar grassland, paddocks were grazed or rested, in all combinations of the four seasons of the year, over a ten year period. Changes in botanical composition and cover of the sward were monitored at regular intervals. The results of these investigations indicate that under a regime of non‐selective grazing the composition of Dohne Sourveld is remarkably stable. After 36 years of continuous grazing the species composition of the sward was largely maintained. Floristically Dohne Sourveld is dominated by a small number of grass species of which Andropogon appendiculatus, Elionurus muticus, Heteropogon contortus, Sporobolus africanus, Themeda triandra and Tristachya leucothrix are the most important. These species react differently to under‐utilization or increasingly severe defoliation, enabling them to be classified into decreaser or increaser categories. Severe defoliation resulted in a marked decrease in basal cover but only small changes in relative species composition. As the intensity of defoliation increases, decrease in grass cover is associated with an increase of forbs, such as Senecio retrorsus.     

Ontogeny of Themeda triandra tillers in the false thornveld of the eastern cape

Abstract

Marked populations of Themeda triandra tillers were sequentially sampled at fortnightly intervals under normal conditions and without moisture stress respectively, covering a period from tiller initiation through to senescence after flowering. Parameters recorded were height of shoot apex, tiller mass, leaf area, leaf number, secondary tiller development and premature tiller mortality. Tillers of irrigated plants had a significantly higher mass, more elevated shoot apices, and produced a greater total number of leaves than those of non‐irrigated plants. Fewer unstressed tillers senesced prematurely than those experiencing moisture stress.     

Ontogeny of sporobolus fimbriatus tillers in the false thornveld of the Eastern Cape

Abstract

Marked populations of Sporobolus fimbriatus tillers were sequentially sampled at fortnightly intervals under normal rainfall conditions and under irrigation respectively, covering a period from tiller initiation through to senescence after flowering. Parameters recorded included shoot apex elevation, tiller mass and leaf development.

Surviving tillers consisted of two sub‐populations, those that flowered in autumn and those that remained vegetative. Flowering of individual apices was rapid, but was spread over a number of months for the whole population. Moisture played an important role in determining tiller size, and at least some forage was lost through leaf drop on tillers rested for a whole season.     

The national grazing strategy of the Republic of South Africa Objectives,achievements and future challenges

Over the past few years various measures have been applied to address the problem of the retrogression of southern African rangelands. However, it has become apparent that many of these measures were ad hoc and not part of a long‐term strategy aimed at fostering effective management of the rangeland resources. The White Paper on Agricultural Policy, tabled in May 1984, made reference to the alarming deterioration of natural rangelands and led to the drawing up of the National Grazing Strategy (NGS), released to Parliament in May 1985, which was endorsed by the Department of Agriculture and accepted in its entirety by the Government. The NGS committed authorities to reassess approaches to rangeland utilization in South Africa and to achieve a number of clearly‐defined goals within a specified time frame. The implementation of the NGS has been carefully monitored. Despite some progress, few of the goals set have actually been achieved. However, various problems have arisen such as the need to: encourage the cooperation of all range users, develop informed public opinion, increase manpower and establish priorities, all of which must be taken into account when future rangeland strategies are devised. These strategies also need to recognize the dichotomy that exists between programmes aimed at the development of certain industries and those aimed at resource conservation. It is proposed that future implementation of the NGS should be augmented by some of the following important second phase actions: (i) The strategy should be converted into a policy to be applied to all Departmental institutions, (ii) policy makers and politicians must accept liability and must take the lead in the application of stringent measures aimed at agricultural resource conservation and utilization.