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Flow cytometric sorting technology was used to measure the difference in DNA content between X- and Y-chromosome-bearing spermatozoa in bucks. Spermatozoa were analysed by flow cytometry to characterize X- and Y-chromosome-bearing sperm populations and to quantify the DNA difference between them. Two symmetrical, overlapping and clearly separated peaks, corresponding to X- and Y-bearing spermatozoa, were detected. The difference in fluorescence intensity between the peaks was 4.4 +/- 0.03% without any significant inter- or intra-animal variations. Therefore, the identification and selection of high-purity samples of sperm populations for sex sorting is easier in bucks compared with other domestic species. 相似文献
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DW Bartlett JM Clough JR Godwin AA Hall M Hamer B Parr‐Dobrzanski 《Pest management science》2004,60(3):309-309
The original article to which this Correction refers was published in Pest Management Science 58 (7): 649–662 (2002).Copyright © 2004 Society of Chemical Industry 相似文献
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Fortes Maira Salomão Lopes-Mori Fabiana Maria Ruiz Caldart Eloiza Teles Constantino Caroline Evers Fernanda Pagliari Sthefany de Almeida Jonatas Campos Barros Luiz Daniel Freire Roberta Lemos Garcia João Luis Headley Selwyn Arlington Navarro Italmar Teodorico 《Tropical animal health and production》2018,50(2):413-419
Tropical Animal Health and Production - This study investigated the prevalence of caprine toxoplasmosis in goat herds from Southern Brazil by the indirect immunofluorescence assay (IFA), and... 相似文献
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de Carvalho Menezes de Almeida Sheylla Foligno Souza-Fabjan Joanna Maria Gonçalves Balaro Mario Felipe Alvarez Bragança Gláucia Mota Pinto Pedro Henrique Nicolau de Almeida José Gabriel Moura Ana Beatriz Bossois da Fonseca Jeferson Ferreira Brandão Felipe Zandonadi 《Tropical animal health and production》2018,50(2):427-432
Tropical Animal Health and Production - This study evaluated the effect of two doses of prostaglandin at different intervals on reproductive parameters of crossbred ewes. In Experiment 1, 30 ewes... 相似文献
7.
Equine pituitary pars intermedia dysfunction: current understanding and recommendations from the Australian and New Zealand Equine Endocrine Group
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CJ Secombe SR Bailey MA de Laat KJ Hughes AJ Stewart JM Sonis RHH Tan 《Australian veterinary journal》2018,96(7):233-242
The purpose of this article is to provide a review of the current knowledge and opinions about the epidemiology, clinical findings (including sequelae), diagnosis, treatment and monitoring of equine pituitary pars intermedia dysfunction, particularly in the Australian context. This information and the recommendations provided will assist practitioners in making informed decisions regarding the diagnosis and management of this disorder. 相似文献
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Paula de Aguiar Silva Gleidson Giordano Pinto de Carvalho Aureliano José Vieira Pires Stefanie Alvarenga Santos Douglas dos Santos Pina Robério Rodrigues Silva Carlindo Santos Rodrigues Luis Henrique Almeida de Matos Carlos Emanuel Eiras Daiane Novais-Eiras Wilson Souza Nunes 《Tropical animal health and production》2018,50(4):721-726
In this study, we evaluated the effects of including cassava wastewater in the diet on the feeding behavior of feedlot lambs in 35 male uncastrated Santa Inês × Dorper crossbred lambs at an approximate age of 3 months, with an average live weight of 20.0?±?3.4 kg. Diets were formulated with hay of cassava shoots (roughage) and a concentrate based on corn and soybean, with a roughage:concentrate ratio of 50:50, plus inclusion of cassava wastewater at the levels of 0, 12, 24, 36, or 48 g/kg of the total diet. Feeding behavior was evaluated between the 46th and 52nd days of the experiment. Increasing cassava wastewater levels in the diet reduced (P?<?0.05) the intakes (kg/day) of dry matter and neutral detergent fiber as well as the efficiency of rumination (g/cud and g/h) of dry matter and neutral detergent fiber. The other behavioral parameters were not affected by wastewater inclusion in the diet. Therefore, the inclusion of up to 48 g/kg of cassava wastewater on fresh matter of diets is not recommended for feedlot lambs. 相似文献
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The purpose of this study was to investigate the effect of seminal plasma (SP) from bulls of known fertility on bovine endometrial epithelial cells (bEEC) in culture. The bEEC from passage 5, approximately 5.0–13 × 105 cells per flask, were challenged with SP from bulls of high or low fertility (n = 3 and 2, respectively) or PBS (control), at 1% (75 μl) or 4% (300 μl) and were incubated for 72 hr (n = 13 per challenge). Total cell number and viability of bEEC after challenge with 1% SP from either high‐ or low‐fertility bulls (75H or 75L, respectively) did not differ from controls. In contrast, challenge with 4% of SP from high‐ or low‐fertility bulls (300H or 300L) negatively affected bEEC cell number and viability. Challenge with 300 L had a greater adverse effect than 300H. These results suggest that the negative effect of bovine SP on bEEC is both dose‐dependent and fertility‐dependent. 相似文献
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Food resources and cyprinid diet in permanent and temporary Mediterranean rivers with natural and regulated flow
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Carlos M. Alexandre Sabrina Sales Maria Teresa Ferreira Pedro R. Almeida 《Ecology of Freshwater Fish》2015,24(4):629-645
This study addresses the differences in food availability, diet and feeding activity of the Iberian barbel, between permanent and temporary nonregulated rivers, and the effect of flow regulation on feeding parameters. A total of 267 adult barbels were seasonally collected in four nonregulated and regulated rivers from permanent and temporary basins, and their gut content was analysed. Locally available food sources were evaluated across sites and seasons. Barbels from the permanent nonregulated river exhibit a more variable and diversified diet in which invertebrates assumed a large importance, especially during high flows. Barbels from the temporary nonregulated river presented a more uniform diet composed of plant material and detritus, particularly in drought seasons. Flow regulation affected different flow components in both systems, but the effects on food resources and barbels' diet were similar, resulting in an intra‐annual stabilisation of resource availability and fish diet, with a higher consumption of plants and detritus. Changes in fish diet and feeding activity in both nonregulated and regulated rivers were strongly associated with the seasonal variability of streamflow components, particularly between low‐ and high‐flow periods, and with the reduction in flow variability in the case of dam regulation. Results from this study can be used to improve guidelines for flow requirement implementation. 相似文献