Weather-Centric Rangeland Revegetation Planning

Invasive annual weeds negatively impact ecosystem services and pose a major conservation threat on semiarid rangelands throughout the western United States. Rehabilitation of these rangelands is challenging due to interannual climate and subseasonal weather variability that impacts seed germination, seedling survival and establishment, annual weed dynamics, wildfire frequency, and soil stability. Rehabilitation and restoration outcomes could be improved by adopting a weather-centric approach that uses the full spectrum of available site-specific weather information from historical observations, seasonal climate forecasts, and climate-change projections. Climate data can be used retrospectively to interpret success or failure of past seedings by describing seasonal and longer-term patterns of environmental variability subsequent to planting. A more detailed evaluation of weather impacts on site conditions may yield more flexible adaptive-management strategies for rangeland restoration and rehabilitation, as well as provide estimates of transition probabilities between desirable and undesirable vegetation states. Skillful seasonal climate forecasts could greatly improve the cost efficiency of management treatments by limiting revegetation activities to time periods where forecasts suggest higher probabilities of successful seedling establishment. Climate-change projections are key to the application of current environmental models for development of mitigation and adaptation strategies and for management practices that require a multidecadal planning horizon. Adoption of new weather technology will require collaboration between land managers and revegetation specialists and modifications to the way we currently plan and conduct rangeland rehabilitation and restoration in the Intermountain West.     

高吞吐量协作防火墙的双向去冗余方法

针对协作防火墙中冗余规则会降低其吞吐量的问题,提出一种基于双向去冗余的高吞吐量协作防火墙优化方法.该方法通过比对协作防火墙中双方的防火墙规则,双向去除域内防火墙之间的冗余规则,提高整个协作防火墙的数据包过滤能力.仿真实验结果表明,该方法的冗余率达到了22.7%,提高了8.2%吞吐量,明显优于已有协作防火墙优化方法.     

Isolates of Verticillium dahliae Pathogenic to Crucifers Are of at Least Three Distinct Molecular Types

ABSTRACT Diverse isolates of the soilborne wilt fungi Verticillium dahliae and V. albo-atrum were studied to understand the nature and origins of those infecting cruciferous hosts. All isolates from cruciferous crops produced microsclerotia, and the majority produced long conidia with a high nuclear DNA content; these isolates were divided into two groups by amplified fragment length polymorphism (AFLP) analysis. One group could be subdivided by other criteria such as rRNA sequences and mitochondrial DNA restriction fragment length polymorphism (RFLP) analysis. Two crucifer isolates were short spored and had a low nuclear DNA content. The results are consistent with the crucifer isolates being interspecific hybrids. The long-spored isolates are best regarded as amphihaploids (or allodiploids) with the AFLP groups probably each representing separate interspecific hybridization events. The short-spored crucifer isolates appear to be derived from interspecific hybrids and are here called 'secondary haploids'. Molecular evidence suggests that one parent in the crosses was similar to V. dahliae. The other parent of the amphihaploids seems to have been more similar to V. albo-atrum than to V. dahliae, but was distinct from all isolates of either species so far studied. The implications for the taxonomy of crucifer isolates are discussed and the use of the name V. longisporum, proposed elsewhere for just some of these isolates, is discouraged.     

Effect of a Bowman-Birk proteinase inhibitor from Phaseolus coccineus on Hypothenemus hampei gut proteinases in vitro

The coffee berry borer, Hypothenemus hampei (Ferrari), is an important devastating coffee pest worldwide. Both trypsin and chymotrypsin enzyme activities from H. hampei larval midgut can be inactivated by proteinaceous enzyme-inhibitors. A serine proteinase inhibitor belonging to the Bowman-Birk class was purified from a wild accession of Phaseolus coccineus L. seeds. The inhibitor (PcBBI1) is a cysteine-rich protein that is heat-stable at alkaline pH. MALDI-TOF/MS analysis showed that PcBBI1 occurs in seeds as a monomer (8689 Da) or dimer (17,378 Da). Using in vitro inhibition assays, it was found that PcBBI1 has a high inhibitory activity against H. hampei trypsin-like enzymes, bovine pancreatic chymotrypsin, and trypsin. According to this, PcBBI1 could be a promising tool to make genetically modified coffee with resistance to coffee berry borer.     

Liming as an ameliorator of copper toxicity in black oat (Avena strigosa Schreb.)

This study aimed to assess the effect of liming as an ameliorator of copper (Cu) toxicity in black oats (Avena strigosa Schreb.) grown in soil with a sandy texture. The treatments were soil supplementation with increasing doses of lime (0.0, 0.5 and 1.0 g kg^?1) and two of Cu (0 and 50 mg kg^?1). Pre-germinated black oat seeds were sown for 30 days in the soil, subsequently, the plant height, root and shoot dry mass and concentration of total chlorophyll, chlorophylls a and b, carotenoids and total soluble carbohydrates and starch were analyzed. The dose of 50 mg kg^?1 soil Cu caused a toxic effect on black oat plant growth and development, affected the internal distribution of nutrients, decreased the leaf chlorophyll concentration and increased the leaf concentration of total soluble carbohydrates and starch. Increasing lime doses ameliorated the toxic effects of Cu.