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排序方式: 共有277条查询结果,搜索用时 15 毫秒
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GA Presicce S Verberckmoes EM Senatore P Klinc D Rath 《Reproduction in domestic animals》2005,40(1):73-75
At the time of AI following Ovsynch protocol, a total of 51 buffaloes were randomly divided in a first group (n = 30) subjected to conventional AI into the uterine body with 20 million non-sex sorted frozen-thawed spermatozoa, while a second group (n = 21) was inseminated near the utero-tubal junction (UTJ) ipsilateral to the ovary carrying the preovulatory follicle with 2.5 million live (4 million total) sex-sorted frozen-thawed spermatozoa. The semen used for flowcytometric sorting was collected and processed on a farm in Italy, and then shipped to a laboratory in Germany. Eleven buffaloes were inseminated with X-chromosome bearing spermatozoa and 10 with Y-chromosome bearing spermatozoa. Conception rates after conventional and UTJ inseminations were 43.3% (n = 13) and 42.8% (n = 9) respectively (p = 0.97). Eight of the nine foetuses obtained after insemination with sexed spermatozoa corresponded to the sex as predicted by the cell sorting procedure (five male and four female foetuses by ultrasound vs six male and three female foetuses by cell sorting). In conclusion, for the first time buffalo semen has been successfully subjected to procedures for flowcytometric sperm sorting and freezing. Low doses of sexed spermatozoa have been deposited near the UTJ giving conception rates similar to those of conventional AI with full dose. 相似文献
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Effect of Pimobendan in Dogs with Preclinical Myxomatous Mitral Valve Disease and Cardiomegaly: The EPIC Study—A Randomized Clinical Trial
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A. Boswood J. Häggström S.G. Gordon G. Wess R.L. Stepien M.A. Oyama B.W. Keene J. Bonagura K.A. MacDonald M. Patteson S. Smith P.R. Fox K. Sanderson R. Woolley V. Szatmári P. Menaut W.M. Church M. L. O'Sullivan J.‐P. Jaudon J.‐G. Kresken J. Rush K.A. Barrett S.L. Rosenthal A.B. Saunders I. Ljungvall M. Deinert E. Bomassi A.H. Estrada M.J. Fernandez Del Palacio N.S. Moise J.A. Abbott Y. Fujii A. Spier M.W. Luethy R.A. Santilli M. Uechi A. Tidholm P. Watson 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2016,30(6):1765-1779
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Zhao S McDermott PF White DG Qaiyumi S Friedman SL Abbott JW Glenn A Ayers SL Post KW Fales WH Wilson RB Reggiardo C Walker RD 《Veterinary microbiology》2007,123(1-3):122-132
Three hundred and eighty Salmonella isolates recovered from animal diagnostic samples obtained from four state veterinary diagnostic laboratories (AZ, NC, MO, and TN) between 2002 and 2003 were tested for antimicrobial susceptibilities and further characterized for bla(CMY) beta-lactamase genes, class 1 integrons and genetic relatedness using PFGE. Forty-seven serovars were identified, the most common being S. Typhimurium (26%), S. Heidelberg (9%), S, Dublin (8%), S. Newport (8%), S. Derby (7%), and S. Choleraesuis (7%). Three hundred and thirteen (82%) isolates were resistant to at least one antimicrobial, and 265 (70%) to three or more antimicrobials. Resistance was most often observed to tetracycline (78%), followed by streptomycin (73%), sulfamethoxazole (68%), and ampicillin (54%), and to a lesser extent chloramphenicol (37%), kanamycin (37%), amoxicillin-clavulanic acid (20%), and ceftiofur (17%). With regards to animal of origin, swine Salmonella isolates displayed the highest rate of resistance, being resistant to at least one antimicrobial (92%), followed by those recovered from turkey (91%), cattle (77%), chicken (68%), and equine (20%). Serovars commonly showing multidrug resistance (MDR) to > or =9 antimicrobials were S. Uganda (100%), S. Agona (79%), and S. Newport (62%), compared to S. Heidelberg (11%) and S. Typhimurium (7%). Class-1 integrons were detected in 43% of all isolates, and were found to contain aadA, aadB, dhfr, cmlA and sat1 gene cassettes alone or in various combinations. All ceftiofur resistant isolates (n=66) carried the bla(CMY) beta-lactamase gene. A total of 230 PFGE patterns were generated among the 380 isolates tested using XbaI, indicating extensive genetic diversity across recovered Salmonella serovars, however, several MDR clones were repeatedly recovered from different diseased animals. 相似文献
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C H Vane S C Martin C E Snape G D Abbott 《Journal of agricultural and food chemistry》2001,49(6):2709-2716
The oyster mushroom (Pleurotus ostreatus) is widely cultivated on wheat straw (Triticum aestivum); however, there is a need to better understand the relationship between the chemical composition of the compost and mushroom growth. Wheat straw was degraded over a period of 63 days by P. ostreatus during which time it was sampled at weekly intervals. Off-line thermochemolysis with tetramethylammonium hydroxide and solid-state (13)C NMR were then used in the molecular characterization of the undegraded wheat straw and the degraded samples. The degraded wheat straw samples had a lower proportion of syringyl- to guaiacyl-derived moieties and cinnamyl- to guaiacyl-derived moieties than the undegraded control. There were increases in both guaiacyl and syringyl acid to aldehyde ratios with composting time, which showed that side-chain oxidation has been mediated by P. ostreatus. The (13)C NMR spectra confirmed the increase in carboxyl content but indicated that the overall lignin and methoxyl contents remained relatively constant, although some nonsystematic variations were observed. The spectra also showed a decrease in amorphous noncellulosic polysaccharides in relation to the crystalline cellulose upon degradation. 相似文献
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A method for the separation of the oil, emulsifier and polymer or salt fractions of adjuvants is described. The adjuvant is dissolved in 90% aqueous ethanol and filtered to remove polymers or salt. Light petroleum (distillation range 30–40°C) is added to the solution in a separating funnel. The light petroleum fraction is subsequently extracted with successive aliquots of 80, 70, 60 and 50% aqueous ethanol. The whole of the ethanol and light petroleum fractions are evaporated to dryness to yield the emulsifier and oil fractions respectively. Emulsifier, oil and polymer or salt are determined gravimetrically. The method successfully detected the presence of polymer or salt in adjuvants, but could not distinguish between them when both were present in the same product. The adjuvants could be classified into four broad classes; those containing > 70% emulsifier (wetter-spreaders), those containing > 75% oil (anti-evaporants), those containing a high proportion of polymer (film-forming substances), and those containing comparable amounts of all three components (multi-purpose adjuvants). 相似文献
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Fruit-set of ‘Golden Delicious’ apple trees was found to be largely independent of seasonal differences and much more closely related to the initial number of blossom clusters, when compared with many other apple cultivars under English conditions. In one young orchard, yield became increasingly a function of trunk girth during the first 3 cropping-years, but the trees then abruptly developed a biennial rhythm. Thereafter, blossom production was negatively correlated with the number of clusters in the previous year rather than with the number of fruits.Applications of daminozide or GA3 appeared to increase or decrease, respectively, the amount of return bloom without altering the negative correlation with the number of clusters in the previous year. Chemical fruit-thinning with a mixture of carbaryl and NAA also appeared to be effective in increasing return bloom. 相似文献
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