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Riaz Aayesha Yousaf Arfan Moaeen-ud-Din Muhammad Shah Muhammad Ali Abdullah Zainab Tayyaba Masood Sadia Akhter Naeem Ali Adnan 《Veterinary research communications》2019,43(4):197-202
Veterinary Research Communications - Avian polyomavirus (APV) infection, also called as budgerigar fledgling disease (BFD) causes various health problems in many psittacine species which may cause... 相似文献
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Chaudhry Mamoona Ahmad Maqbool Rashid Hamad Bin Sultan Bakhat Chaudhry Haroon Rashid Riaz Aayesha Shaheen Muhammad Shabir 《Tropical animal health and production》2017,49(1):213-220
Tropical Animal Health and Production - A prospective study was conducted from November 2013 to February 2014 to estimate the spatial clustering; cumulative incidence and risk factors associated... 相似文献
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Aayesha Riaz Inga Dry Robert Dalziel Saif Ur Rehman Muhammad Ali Shah Hafiz Muhammad Naeem Akhtar Arfan Yousaf Ruqia Baig 《Journal of veterinary science (Suw?n-si, Korea)》2021,22(4)
BackgroundMalignant catarrhal fever (MCF) is a highly fatal lymphoproliferative disease of cattle, deer, bison, water buffalo, and pigs caused by the gamma-herpesviruses alcelaphine herpesvirus-1 (AlHV-1) and ovine herpesvirus-2 (OvHV-2).ObjectivesThis study aimed to determine the prevalence of OvHV-2 in sheep, goats, cattle, and buffalo in Rawalpindi and Islamabad, Pakistan, by applying molecular and phylogenetic methods.MethodsBlood samples were aspirated from sheep (n = 54), goat (n = 50), cattle (n = 46) and buffalo (n= 50) at a slaughterhouse and several farms. The samples were subjected to heminested polymerase chain reaction (PCR), followed by sequencing and phylogenetic analysis of the OvHV-2 POL gene and the OvHV-2 ORF75 tegument protein gene.ResultsThe highest percentage of MCF positive samples was in sheep (13%), whereas goat, cattle, and buffalo had lower positive percentages, 11%, 9%, and 6.5%, respectively. Four OvHV-2-positive PCR products obtained from sheep samples were sequenced. The sequences obtained were submitted to the NCBI GenBank database ( for the POL gene; MK852173, MK840962, and MK852171 for the ORF75 tegument protein gene). Phylogenetic analysis revealed a close similarity of study sequences with those of worldwide samples.ConclusionsThis study is the first cross-sectional study on the prevalence and molecular detection of OvHV-2 in apparently healthy cattle and buffalo that could be carrying OvHV-2 acquired from OvHV-2-positive sheep and goats. The results indicate that OvHV-2 is circulating in Pakistan. Further studies are needed to characterize OvHV-2 and elucidate further its prevalence. MK852172相似文献
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