肥大细胞研究概述

肥大细胞是机体内重要的效应性和调节性免疫细胞,与巨噬细胞、树突状细胞、自然杀伤细胞等共同构成机体的第一道防线.随着近年来对肥大细胞研究的不断深入,发现肥大细胞除了参与机体的过敏反应外,还参与了机体的多种生理病理过程,还可能与中医的经络穴位和针灸效应的产生存在着密切的联系.作者主要从肥大细胞的产生、功能、局部募集、脱颗粒机制等方面概述一下国内外对肥大细胞的研究现状.     

3种分离小鼠腹腔肥大细胞方法的研究

In order to get mast cells in mice abdominal cavity,we used three Percoll gradient separation methods to extract mast cells from mice abdominal cavity,and counted cell viability, cell purity and the total number of cells.The 3 mL Percoll gradient separation liquids in the centrifugal tube from top to bottom were divided into 4 layers to analyze the dynamic distribution of mast cells.The results showed that in the method Ⅰ,the cell viability, cell purity and the total number of cells were (83.51±14.00)%，(69.04±11.75)% and（10.60±5.20）×10⁵ /mL,respectively；in the method Ⅱ,the cell viability, cell purity and the total number of cells were (85.71±9.23)%，(87.10±3.93)% and（11.64±5.73）×10⁵ /mL,respectively；in the method Ⅲ,the cell viability, cell purity and the total number of cells were (72.25±24.81)%，(68.34±10.20)% and（8.87±5.18）×10⁵ /mL,respectively.The cell viability and total number of cells in the three groups were not significantly different(P＞0.05).The cell purity of method Ⅱ was extremely significantly higher than that of methods Ⅰ and Ⅲ (Ρ＜0.01). The dynamic distribution of mast cells in the Percoll gradient separation liquid was that the cell viabilities of 3,4 layers were extremely significantly higher than 1 layer (Ρ＜0.01),significantly higher than 2 layer (Ρ＜0.05); the cell purity of 3 layer was extremely significantly higher than 1,4 layers (Ρ＜0.01), significantly higher than 2 layer (Ρ＜0.05); the number of cells of the 3,4 layers were extremely significantly higher than 1,2 layers (Ρ＜0.01). Therefore, adopting the method Ⅱ, layer 3 of extraction, the middle and lower 0.5 mL, which was close to the interface parts in 30%∶ 80% of the Percoll gradient separation liquid, the separation of mast cells of mice peritoneal cavity was best.     

中药多糖免疫作用概述

天然中草药是我国传统医学的宝贵财富,我国劳动人民几千年来在与疾病作斗争的过程中,通过实践,不断认识,逐渐积累了丰富的医药知识和经验。而随着现代研究的不断发展,发现中药对机体有很好的免疫调节作用,其中起免疫调节作用的主要成分之一就是各种中药多糖〔1～3〕,它与维持生命的种种生理机能有着密切联系。1中药多糖的研究现状