一例猪繁殖与呼吸综合征和链球菌混合感染的诊治

正1发病情况2013年3月21日,武鸣县某猪场就猪病来求诊,并送检患病仔猪5头,发病母猪和仔猪的血液样品各4份。该猪场存栏42头母猪,断奶仔猪132头。3月上旬有8头母猪发病,表现为呼吸急促,经用抗菌、退热等药物对症治疗一周后病情没有好转,发病率升高,并且有19头仔猪死亡,妊娠母猪早产、产死胎和弱仔。该猪场之前仅进行过猪瘟、猪伪狂犬病疫苗免疫。     

伊氏锥虫变异表面糖蛋白抗原双抗体夹心ELISA检测方法的建立及初步应用

A double antibody sandwich ELISA for detection of Trypanosoma evansi variant surface glucoprotein (VSG) antigen was established by using two monoclonal antibodies against Trypanosoma evansi VSG antigen.The result of sensitivity showed when positive serum was diluted to 3200 doubles, its D_{450 nm} value was still greater than the critical value of positive and negative,and specificity test result showed that there was no cross reaction with the antigens of Theileria,Toxoplasma gondii,Chlamydia and Fasciola gigantica.82 clinical sera were detected by this double antibody sandwich ELISA and its positive rate was 9.76%.The experiment results demonstrated that this double antibody sandwich ELISA for detection of Trypanosoma evansi VSG antigen had good detecting sensitivity and specificity,and could be used as an effective method for clinical detecting buffalo Trypanosoma evansi disease.     

内陆进口肉类指定口岸质量安全风险管控措施探讨

借助河南进口肉类指定口岸郑州和漯河两个查验区及双汇、众品、永达等进口肉类企业实际,探讨和研究内陆地区如何应用海铁、海陆联运方式,通过对进口肉类各环节的风险点分析排查以及配套管控措施研究,来保障进口肉类流程更顺畅、质量有保障,潜在风险被发掘、安全措施能实施。以最大限度地保障人类身体健康和农牧业有序发展。