山羊人造瘤胃瘘管术改进的几点体会

因科研需要，我们于2003年9月对16只青年山羊做了永久性人造瘤胃瘘管手术，术后全部山羊的瘤胃瘘管都保持良好，术后健康状况正常。现总结如下。     

全麻机理的研究进展

全身麻醉(General anesthesla)是麻醉药经吸入、静脉、肌肉、口服或直肠灌注等途径送入入或动物体内，对中枢神经系统产生广泛的抑制作用，从而使入或动物呈现可逆的感觉和意识消失、痛觉迟钝或消失、神经反射及肌肉活动不同程度受到抑制的一种状态，这种麻醉方法称为全身麻醉。     

涂饰工艺对木质体育地板表面滑动摩擦系数的影响研究

采用正交试验法,研究涂饰工艺对木质体育地板表面滑动摩擦系数的影响,试验因素为面漆涂饰次数、涂料种类和面板砂光目数,试验测试指标为表面滑动摩擦系数。方差分析结果表明,涂料种类与测试指标呈极为显著的影响关系,水性面漆涂饰的试件表面滑动摩擦系数相对较高;其他2个因素与测试指标之间无显著影响关系。砂光目数极可能对表面滑动摩擦系数有一定影响,但这一影响关系被底漆涂饰工艺所覆盖,因此所有试件的表面滑动摩擦系数全部达到了国家标准的要求。     

高表达线粒体融合蛋白2(MFN2)可抑制高BHBA活化的奶牛肝细胞NF-κB炎性通路

为研究线粒体融合蛋白2(mitofusin 2,MFN2)对高β-羟丁酸(β-hydroxybutyrate,BHBA)活化NF-κB炎性通路的影响,体外培养奶牛肝细胞,添加不同浓度(0.0,1.2,2.4,4.8 mmol/L)的BHBA,并转染过表达MFN2的腺病毒,运用Western blot和qRT-PCR技术检测NF-κB炎性通路关键分子的基因和蛋白表达。结果显示,随着BHBA浓度的增加,IKBα和NF-κB p65的磷酸化水平以及IL-1β、IL-6和TNFα的mRNA表达均显著升高,MFN2的基因和蛋白表达水平则显著降低;过表达MFN2后,显著抑制了高BHBA诱导的IKBα和NF-κB p65磷酸化水平及IL-1β、IL-6、TNFαmRNA表达水平的升高。结果表明,在奶牛肝细胞中过表达MFN2可以显著抑制高BHBA活化的NF-κB炎性通路。     

Improvement of Appearance Traits of elite Rice Zhenshan 97B by Molecular Introgression of Targeted about 800 kb Interval from Indica Rice Jiafuzhan Genome

Rice appearance trait is mainly mean grain length (GL), grain width (GW), ratio of length and width (L/W), chalkiness (CH) and transparence (TR). Appearance is the first impression, and rice appearance traits effect significantly the value of commodity. Hybrid rice is relatively low quality, especially on rice appearance quality,which restricts partially its extend and badly export of hybrid seed and rice in China.Shanyou 63, the most famous hybrid rice combination of China, could not be also sustainable application because of its poor qualities. In this studies, We attempt to improve appearance quality of the elite rice maintainer of Zhenshan 97B by using molecular approach of genomic interval targeted introgression. Three F2 populations were constructed to study the classical and molecular genetic of rice appearance traits, at the same time, molecular marker-asisted toimprove the main maintainer line, Zhenshan 97B were performed. There some following results:Three F2 populations were constructed based on crosses of Guangluai 4×Jiafuzhan (GJ popu.), Minghui 86×Jiafuzhan (MJ popu.) and Zhenshan 97B×Jiafuzhan (ZJ popu.). ZJ popu and GJ popu were produced to reconstruct the linkage maps related with rice appearance traits based on BSA, and detect QTL of target traits based on IM.In ZJ popu, 9 QTLs of target traits were detected. The interval, RM169～RM516 on Chromosome 5, was genetic locus of GW, L/W, CH and BW, and the explained variances were 10.9%, 14.9%, 12.0% and 14.2%, respectively.RM214 on chromosome 7, RM339 on chromosome 8 were genetic loci if CH and BW, and the explained variances were 9.4%, 10.0%, 11.0% and 12.1%. besides there is a genetic locus, RM347, of G/W on chromosome 3, and the explained varance was 10.3%. In GJ popu, 5 QTLs of rice appearance traits were detected. The interval, RM169-RM516 on Chromosome 5, were also the genetic loci of GW, CH and BW, and the explained variances were 9.0%, 12.5%, and 13.6%, respectively. RM264～RM80 on chromosome 8 was the genetic loci of GW amd L/W,and the explained variances were 14.7% and 13.4%. The genotypes of all QTLs, except RM214, from Jiafuzhan contributed to improving riee appearance traits.The research was to improve the rice appearance quality of Zhenshan 97B by introgressing QTLs of rice appearance traits from Jiafuzhan, a donor parent, by means of MAS in the process of recurrent backcrossing, 4 SSR markers were used to detect the target genotypes from donor parent, and 70 SSR primers to identify the genetic background of recurrent parent. Consequently, there were 19 target individuals in BC3, in which the mean value of GL, GW, L/W, CH and BW were 6.06±0.04mm, 2.50±0.02mm, 2.43±0.02, 29.5±2.7 and 29.4±2.7%, respectively,and the mean homologous degree (HD) to recurrent parent is up to 98.22%. Compared to Zhenshan 97B, GL, CH and BW decreased 0.17 mm, 34.9% and 34.5% respectively and however L/W increased 0.19. which accounted separately for 25.37%, 54.79%, 54.57% and 10.50% of thedifference of every trait between two parents. It is very luckly to find a individual, 13BC2-3BC3 line, whose GL, GW, G/W, CH and BW were 6.04 mm, 2.43 mm, 2.49,2.5% and 2.5% separately, There is very important signification for it to be used in hybrid rice breeding improving appearance traits. In the research, MAS efficiency of 3 loci (with 4 markers ) was 5.68% and coincident degree was 73.08%.     

犬子宫蓄脓的诊断及手术治疗报告

<正>犬的子宫蓄脓指犬的子宫腔内贮留大量脓汁并伴有子宫内膜囊泡性增生的疾病称犬的子宫蓄脓,是发情后期的一种疾病,一般分为开放型和闭锁型子宫蓄脓2种。自2006年8月至2007年9月期间,在安丘市畜牧局宠物门诊共收治5例患有子宫蓄脓的小型犬,分别为京巴3例,博美和西施     

马铃薯新品种—秦芋32号选育

秦芋32号(原代号:康0102-5)是2000年以秦芋30号作母本,89-1优系作父本,有性杂交获得实生种子,经育种圃、鉴定圃选育而成。2011年3月申报国家农作物品种审定委员会待审,2011年10月通过审定。2007～2009年参加国家级马铃薯品种中晚熟西南组繁种及区域试验;2010年国家西南组生产试验平均产量1 443 kg/667 m2,比对照米拉增产12.5%。块茎干物质含量18.5%、淀粉含量11.8%,Vc含量14.2 mg/100 g,还原糖含量0.29%,粗蛋白含量2.1%。抗病性接种鉴定:中抗PVX和PVY,抗晚疫病。     

茶园滴灌施肥的增产提质及土壤养分效应研究

以常规施肥作为对照,设计3个不同氮素用量(450、300、150 kg/hm2),研究了茶园滴灌施肥对茶叶产量、品质及土壤养分性状的影响。结果表明,滴灌施肥处理比对照增产3.4%～9.3%,施氮量与产量间呈明显正相关,以年施氮量300～450 kg/hm2产量较高,滴灌施肥对茶叶品质影响不显著。滴灌施肥处理成熟叶含氮量显著高于对照,且季节性差异明显,秋茶﹥夏茶﹥春茶,滴灌施肥可促进茶树对氮素的吸收利用。施肥方式对土壤pH值影响显著,滴灌施肥处理土壤pH值明显高于对照,可能因其氮素利用率较高而有助于减弱无机氮对土壤的酸化作用。     

室内绿化植物应用发展初探

阐述了室内绿化的发展历程、主要功能以及室内绿化植物应用的种类、布置方式、摆放手法,提出室内绿化植物应用的发展方向与措施.