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Maria Rowena R. Romana-Eguia Minoru Ikeda Zubaida U. Basiao Nobuhiko Taniguchi 《Aquaculture International》2010,18(6):1205-1221
Growth of several genetically improved Nile tilapia (GIFT or Genetically Improved Farmed Tilapia, FaST or Freshwater Aquaculture
Center Selected Tilapia, SEAFDEC-selected) and domesticated red tilapia (BFS or Binangonan Freshwater Station, NIFI-red or
National Inland Fisheries Institute red, HL or Hacienda Luisita) stocks were compared in controlled (tank) and uncontrolled
farm conditions (lake-based cages) with unselected NIFI or Chitralada Nile tilapia as control. Specific growth rate differed
significantly (P = 0.009) in tank-reared Nile tilapia stocks where GIFT grew best at 1.358%/day followed by FaST (1.307%/day), control stock
NIFI (1.257%/day) and SEAFDEC-selected (1.202%/day). Genetic effect explained 84.4% of the variance in growth of Nile tilapia
in tanks. Although Nile tilapia growth in cages followed the same trend where GIFT grew best at 1.570%/day, no significant
stock differences (P = 0.479) were noted. Meanwhile, red tilapia reared in either tanks or cages showed no significant stock differences in terms
of growth. However, survival of the red tilapia stocks in cages differed significantly with HL having the highest percentage
survival at 93.3%. The different growth responses of the Nile tilapia stocks especially under controlled (tank) farm conditions
were largely due to genetic factors (stock differences).Under uncontrolled farm conditions, environmental factors were generally
observed to have also affected the survival and to some extent, the growth of Asian Nile and red tilapia stocks. 相似文献
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Growth response of Nile tilapia fry to salinity stress in the presence of an 'internal reference' fish 总被引:1,自引:0,他引:1
Growth of three strains of Oreochromis niloticus L. fry exposed to salinity stress in the presence of an internal reference fish were compared. The Central Luzon State University (CLSU) strain was obtained from the Freshwater Aquaculture Center, CLSU, Philippines. The ISRAEL strain was acquired from the Philippine government's Bureau of Fisheries and Aquatic Resources National Freshwater Fisheries Technology Center (BFAR‐NFFTC), Munoz, Nueva Ecija. The National Inland Fisheries Institute (NIFI) strain was obtained from the NIFI, Bangkok, Thailand. Eight to nine full‐sib families (replicates) per strain were split into two groups. One group was grown in freshwater for 2 weeks, acclimated to 32 ppt and reared for 2 weeks and finally grown in freshwater for another 2 weeks. Another group was contemporaneously grown in freshwater polyethylene tanks for 6 weeks. Each replicate family included a size‐matched internal reference population of red tilapia strain. Two‐way analysis of variance (anova ) revealed no significant strain differences (P=0.081; r2=0.106). However, analysis of covariance with the internal reference strain used as a covariate showed significant (P=0.049; r2=0.638) strain effects on specific growth (based on standard length measurements). The ISRAEL strain showed consistently better growth rate in both saline and freshwater environments than the NIFI and CLSU strains. We estimated the statistical power of the two‐way anova (?=√(k′?1)(factor MS?s2)/(k′s2); Zar 1984) to be ~0.30. There was a 70% probability of a Type II error and no true difference in the growth of the three strains was detected. The use of internal reference strain as a covariate improved the r2 from 0.106 to 0.638 and increased the efficiency of the test in detecting a true difference. Other strain comparison studies in our laboratory at the Southeast Asian Fisheries Development Center Aquaculture Department showed that the ISRAEL strain shows better growth than the NIFI and CLSU strains in a crowding stress tolerance experiment, when fed only with rice bran and under restrictive feeding regimes. 相似文献
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Maria Rowena R Romana-Eguia Minoru Ikeda Zubaida U Basiao & Nobuhiko Taniguchi 《Aquaculture Research》2005,36(1):69-78
Two control (C1 or first control generation, and C4 or fourth control generation) and three selected (S1 or first selected generation, S2 or second selected generation, S4 or fourth selected generation) stocks of Chitralada Nile tilapia were analysed for microsatellite variation to determine the effect of size‐specific mass selection on genetic variability. Genetic variation based on five microsatellite loci (UNH123, UNH147, UNH172, UNH222 and UNH216) showed a slightly higher allelic diversity in the selected stocks (7.4–10 alleles) than in the control stocks (6.8–8.8 alleles). Apparent reductions in the mean number of alleles and He values were noted in successive generations of both control and selected lines. Significant deviations from Hardy–Weinberg equilibrium because of an excess of homozygotes indicated inbreeding in all control and selected stocks. Although estimated inbreeding levels were not significantly different among selected and control lines based on Welch's t‐tests, the increase in the degree of inbreeding within the selected line was higher (107.9%) than the control line (64.2%) after four generations. The implications of these results on the management and conservation of genetic diversity in improved breeds are discussed, while the importance of monitoring and minimizing inbreeding are likewise emphasized. 相似文献
4.
Genetic assessment of milkfish (Chanos chanos Forsskal) stocks based on novel short tandem repeats for marker‐aided broodstock management 下载免费PDF全文
Maria Rowena R Romana‐Eguia Brian S Santos Minoru Ikeda Zubaida U Basiao Akihiro Kijima 《Aquaculture Research》2018,49(4):1557-1568
Milkfish hatchery broodstock are either from on‐grown wild‐caught or hatchery‐produced fry/juveniles. To determine if a marker‐assisted management scheme can be formulated for improved milkfish hatchery production, milkfish stocks were genetically characterized using nine novel short tandem repeats or microsatellites. Eight wild‐bred Philippine stocks (CLA, CUR, CAM, SIH, SBH‐I1, HH, PAL and ZH‐P0), four hatchery‐bred stocks (SBH‐I2, SBH‐D, BoH and ZH‐F1), two farm stocks of known mixed lineages (SPH and BDH) and one Indonesian hatchery‐bred stock (WJH) were assessed. WJH was included since milkfish fingerlings from Indonesia reared in Philippine farms could be developed into future broodstock. Mean allelic richness (Ar) was highest in wild‐bred stocks (9.5) and lowest in hatchery‐bred spawners (9.1). Mean expected heterozygosities (He) were relatively similar in all stocks with wild‐bred stocks slightly higher (0.67) than the others. An analysis of molecular variance indicated significant yet low genetic differentiation among stocks (FST = 0.013; p = .000) where variation (98.6%) was explained by intra‐stock differences. In some of the domesticated stocks, reductions in mean allelic richness were observed in first generation hatchery broodstock (e.g. ZH‐F1; Ar = 8.3), compared with their founder stock (e.g. ZH‐P0; Ar = 9.4). The Indonesian stock was similar to local wild‐bred stocks based on genetic variability indices; thus, it might be likely that the local stocks’ fitness traits could be comparable with the imported milkfish stock which has been perceived to be better. The quality of locally available farmed milkfish and prospects of formulating a broodstock management scheme for the production of good quality milkfish seedstock are herewith discussed. 相似文献
5.
A farmer-oriented Nile tilapia, Oreochromis niloticus L., breed improvement in the Philippines 总被引:1,自引:0,他引:1
Opportunities for developing small‐scale tilapia industry in the Philippines is hampered by the shortage of good‐quality seeds and broodstock. Most small‐scale farmers are dependent on distribution centres for improved tilapia seeds that are expensive and not sufficient to meet market demands. An option would be for farmers to develop their own tilapia breeds using simple procedures within their technical and financial resources. This option will also help sustain the diversity of locally adapted domestic stocks of tilapia. The Philippine tilapia production of ~122 316 MT can be increased by ensuring a stable supply of quality seeds and transferring suitable technology to fish farmers. The study was carried out in a tilapia hatchery/nursery pond in the Philippines to explore the potential for a farmer‐based research on tilapia breed improvement using relatively simple artificial selection procedures. One generation of size‐specific mass selection based on the early culling of large fry (collimation procedure) was applied on a Nile tilapia strain, Oreochromis niloticus L., in net cages set in a small earthen pond. Two episodes of directional selection were performed after initial removal of large fry at 21 days. Selection of parents and progeny testing were conducted in hapa and B‐net cages set in earthen ponds. The selection resulted in a significant response of 8% for standard length and 29% for weight relative to the control. The crude estimates of realized heritability is ~16% for standard length and ~26% for weight comparable with similar studies conducted by other workers. 相似文献
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