In vitro selection of tomato transformants at the immature embryo stage

Summary A method is described for selecting resistant transformed tomato genotypes in vitro at the stage of immature embryos. The utilization of HLH nutrient medium with the selective agent kanamycin is proposed. Normal development of seedlings from immature embryos, which do not form callus, is a good and true indicator for isolation of resistant genotypes. The immature embryos do not germinate and develop on MS selective medium.     

Rhabdias kongmongthaensis sp. n. (Nematoda: Rhabdiasidae) from Polypedates leucomystax (Amphibia: Anura: Rhacophoridae) in Thailand

Rhabdias kongmongthaensis sp. n. is described based on specimens found in the lungs of the tree frog Polypedates leucomystax (Gravenhorst) (Amphibia: Rhacophoridae) from Kanchanaburi Province, western Thailand. The new species is similar to two North-American species, Rhabdias ranae and R. americanus, by presence of two lateral pseudolabia, each with two inner submedian protuberances. R. kongmongthaensis differs from both species by relative length and shape of the tail, and by its distribution and host specificity. Presence of lateral pseudolabia distinguishes the new species from the geographically closest Rhabdias species as well as from those parasitizing other rhacophorid frogs.     

用于伏尔加河地区矮秆春小麦育种的Rht基因的研究和选择

对小麦品系Ｓａｒａｔｏｖｓｋａｙａ２９的不同Ｒｈｔ等位的一套近等基因系进行了田间试验,以测定６个矮杆基因的育种值。在不灌溉、灌溉并施用不同剂量的肥料（Ｎ１２０,Ｐ１００,Ｋ６０和Ｎ１８０,Ｐ１６０,Ｋ１００）三种条件下测定Ｒｈｔ基因对株高、产量及产量构成因子、品质的影响。结果表明,Ｒｈｔ基因可以用于伏尔加河地区条件下魏杆小麦品系的选育。     

Maize (Zea mays L.) yield and soil properties as affected by no tillage in the black soils of China

A four-year tillage experiment on maize was conducted in the high latitude region of Northeast of China. The main objective of this study was to investigate the effects of different field tillage practices on maize grain yield, soil physical properties, and soil water and temperature dynamics in Northeast of China. The tillage practices included moldboard plow tillage (MOT), rotary tillage (ROT), reduced tillage (RET), combined tillage (COT), and no tillage (NOT). The surface soil water content at depths of 0–20 cm was higher under NOT compared with other tillage practices, but not different at the deeper soil depths in 2011. The soil temperatures under NOT and RET were lower than those under moldboard plow tillage and ROT at depths of 5 and 15 cm, respectively, measured at 9:00 am in 2005. From the hourly dynamics of soil temperature, the differences among the tillage practices mainly appeared during the daytime (from 8:00 am to 7:00 pm). Among all the practices, the average daily soil temperature under ROT was the highest, while that under NOT was the lowest. MOT, ROT, and RET had higher soil accumulative infiltration compared with NOT and COT. The surface soil bulk density under NOT was higher than or equal to that under the other four tillage practices. The maize yields under NOT were the lowest among all the tillage practices for three years straight. Meanwhile, the yields under MOT were the highest, which were about 47%, 61%, and 38% higher than those under NOT. NOT practice is not recommended for use in spring-planted maize under the high latitude humid cool climate Mollisol region in Northeast of China.     

Net oxidative addition of C(sp3)-F bonds to iridium via initial C-H bond activation

Carbon-fluorine bonds are the strongest known single bonds to carbon and as a consequence can prove very hard to cleave. Alhough vinyl and aryl C-F bonds can undergo oxidative addition to transition metal complexes, this reaction has appeared inoperable with aliphatic substrates. We report the addition of C(sp(3))-F bonds (including alkyl-F) to an iridium center via the initial, reversible cleavage of a C-H bond. These results suggest a distinct strategy for the development of catalysts and promoters to make and break C-F bonds, which are of strong interest in the context of both pharmaceutical and environmental chemistry.     

Phase modifiers promote efficient production of hydroxymethylfurfural from fructose

Furan derivatives obtained from renewable biomass resources have the potential to serve as substitutes for the petroleum-based building blocks that are currently used in the production of plastics and fine chemicals. We developed a process for the selective dehydration of fructose to 5-hydroxymethylfurfural (HMF) that operates at high fructose concentrations (10 to 50 weight %), achieves high yields (80% HMF selectivity at 90% fructose conversion), and delivers HMF in a separation-friendly solvent. In a two-phase reactor system, fructose is dehydrated in the aqueous phase with the use of an acid catalyst (hydrochloric acid or an acidic ion-exchange resin) with dimethylsulfoxide and/or poly(1-vinyl-2-pyrrolidinone) added to suppress undesired side reactions. The HMF product is continuously extracted into an organic phase (methylisobutylketone) modified with 2-butanol to enhance partitioning from the reactive aqueous solution.     

少免耕土壤结构与导水能力的季节变化及其水保效果

通过对黑土坡耕地免耕、少耕与传统耕作土壤物理性状全生育期观测,比较研究土壤结构和导水性状季节变化差异及其与水土流失的关系。结果表明,表层0~20 cm土壤,免耕土壤容重全生育期维持在1.20~1.30 g cm-3,变化小,大于0.25 mm的水稳性团聚体含量(WR0.25)和平均重量直径(MWD)高于传统耕作,初始和稳定入渗速率均高于少耕和传统耕作,土壤含水量分别较少耕和传统耕作高4.7和4.4个百分点,较传统耕作分别减少地表径流和土壤流失量86%和100%;少耕除夏季各项性状均介于免耕和传统耕作之间,夏季垄沟深松后,垄沟土壤容重显著降低,较免耕和传统耕作降低0.15 g cm-3以上,提高土壤初始入渗速率30%以上,较传统耕作减少水和土壤流失量20%和40%。传统耕作土壤容重,垄台由播种时的0.91 g cm-3增加至收获时的1.23 g cm-3,垄沟一直维持在1.30 g cm-3左右,WR0.25、MWD、土壤稳定入渗速率、含水量均较低,全生育期10%的雨水流失,土壤流失量615 t km-2a-1。免耕土壤结构稳定,蓄水保水最佳,为效果显著的水土保持耕作措施,少耕也有一定的保水保土作用;免耕和少耕均能够改善土壤物理性状。     

Rht基因对春小麦和硬粒小麦离体单雄生殖的影响

研究了离体条件下Ｒｈｔ基因对单雄生殖的影响。选用Ｒｈｔ基因的不同等位基因品系作为抗体材料,包括以砚小麦为背景的Ｒｈｔ１,Ｒｈｔ３、Ｒｈｔ１４及以硬粒小麦Ｃｈａｒｋｏｖｓｋａｙａ ４６为背景的Ｒｈｔ１４和Ｒｈｔ１。结果表明：不同的Ｒｈｔ遗传体系对愈伤诱导频率和植株再生率的影响不同。在砚小麦中,显性Ｒｈｔ５基因对单倍体产生的所有阶段都有明显的正向效应。而在硬粒小麦中,显性的Ｒｈｔ１４基因与其它秭妹相比