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1.
Richard A. Shelby Mediha Yildirim-Aksoy Thomas L. Welker Phillip H. Klesius 《Journal Of Applied Aquaculture》2013,25(1):61-71
Commercially available yeast and yeast subcomponents consisting mainly of β-glucan or oligosaccharide feed additives were added to diets of juvenile (12–18g) Nile tilapia (Oreochromis niloticus) at rates recommended by suppliers. Three experiments were conducted following a basic protocol with varied rates of supplementation, duration of feeding, and stocking densities. Experimental diets were fed twice daily to apparent satiation for a period of two or four weeks, at the end of which feed consumption and weight gain were measured. Following the experimental feeding period, serum components, including protein and immunoglobulin concentrations, as well as lysozyme and complement activities, were measured. A disease challenge was conducted with pathogenic isolates of Streptococcus iniae or Edwardsiella tarda. Weight gains were not significantly different in fish fed the supplemented diets when compared to the control diet. There were significant differences in fed intake within individual experiments; however, this effect was not consistent in all three experiments. Overall feed efficiency was not significantly affected by diet. There were no differences in serum components of fish sampled at two or four weeks. Fish fed the experimental diets did not have lower mortality or morbidity after disease challenge compared to fish fed the control diets. Specific antibody against S. iniae or E. tarda measured by ELISA did not reveal differences in the fish surviving the challenge. We conclude that the incorporation of these commercial yeast component products into the diet of juvenile Nile tilapia at these rates and for these feeding periods had no effect on growth, serum components, antibody responses, or survival following S. iniae or E. tarda infection. 相似文献
2.
Chhorn Lim Julio C. Garcia Mediha Yildirim-Aksoy Phillip H. Klesius Craig A. Shoemaker Joyce J. Evans 《Journal of the World Aquaculture Society》2007,38(2):231-237
This study was conducted to evaluate the effect of dietary levels of distiller’s dried grains with solubles (DDGS) on growth, body composition, hematology, and resistance of Nile tilapia, Oreochromis niloticus, to Streptococcus iniae challenge. Five isocaloric diets containing DDGS at levels of 0, 10, 20, and 40%, and 40% DDGS + lysine (Diets 1–5) as partial replacements of a combination of soybean meal (SBM) and corn meal (CM) on an equal protein basis were fed to juvenile Nile tilapia (9.41 ± 0.14 g) for 10 wk. Fish fed Diet 4 had the lowest weight gain (WG), feed efficiency ratio, protein efficiency ratio (PER), and whole‐body protein. Supplementation of lysine to the 40% DDGS diet (Diet 5) improved WG and PER. Hematological and immunological parameters were not affected by dietary treatment. There were no significant differences among the average number of days to first mortality after S. iniae challenge and cumulative mortality 14 d postchallenge among fish in various treatments. DDGS can be incorporated in tilapia diet at a level of 20% as a substitute for a combination of SBM and CM without affecting their growth performance, body composition, hematological parameters, immune response, and resistance to S. iniae infection. 相似文献
3.
Thomas L. Welker Chhorn Lim Mediha Yildirim-Aksoy Phillip H. Klesius 《Aquaculture (Amsterdam, Netherlands)》2007,262(1):156-162
Juvenile Nile tilapia (Oreochromis niloticus) were fed nutritionally complete, practical basal diets supplemented with bovine lactoferrin (Lf) at 0, 200, 400, 800, or 1600 mg/kg diet to apparent satiation twice daily for 8 weeks. After the feeding trial, the effect of dietary Lf on growth performance, immune function, and resistance to Streptococcus iniae challenge and low-water stress was determined. Dietary Lf did not affect growth performance (weight gain, feed intake, feed efficiency ratio, or survival) or haematological parameters (haemoglobin, white and red blood cell counts, or haematocrit) (P > 0.05). Crowding stress produced significant increases in plasma cortisol, glucose, lactate, and osmolality from baseline values (P ≤ 0.001), but dietary Lf did not affect plasma glucose, osmolality, or cortisol concentrations (P > 0.05). The level of Lf in diet had a significant impact on survival following S. iniae challenge with fish fed the 800 mg/kg Lf diet having significantly higher survival than control fish (P ≤ 0.05). There was not a corresponding increase in activity of non-specific or specific immune parameters (plasma lysozyme and spontaneous haemolytic complement activities or agglutination antibody titer against S. iniae) with addition of Lf to diets (P > 0.05), but plasma iron decreased and total iron binding capacity (TIBC) increased significantly with increasing concentration of Lf in diet (P ≤ 0.05). The ability of Lf to sequester iron, an essential nutrient required for the growth of bacteria, is regarded as one of its key antibacterial properties. The increased survival with increasing dietary concentration of Lf seemed to correspond with a decrease in plasma iron concentration and not enhancement of non-specific or specific immune functions. 相似文献
4.
Thomas L. Welker Chhorn Lim Mediha Yildirim-Aksoy Phillip H. Klesius 《Aquaculture International》2012,20(1):159-176
Juvenile Nile tilapia (Oreochromis niloticus) were fed with diets supplemented with 0 or 6% NaCl for 10 weeks. Tilapia were exposed to approximately 21 mg/l nitrite-N
after 5 and 10 weeks of feeding to determine the effect of dietary NaCl supplementation on resistance to nitrite toxicity.
Fish were sampled before (baseline, pre-exposure) and after 24 h nitrite exposure to determine the effects of dietary NaCl
on mortality, hematology (hematocrit, hemoglobin, and methemoglobin), and plasma electrolyte dynamics (nitrite, chloride,
sodium, and potassium). After 10 weeks of feeding, tilapia were also challenged with Streptococcus iniae to determine the effect of sodium chloride on immunity. Tilapia fed with the NaCl-supplemented diet had significantly higher
weight gain compared with the control group, which was associated with a significant increase in feed intake in the NaCl group.
Mortality from nitrite exposure was lower in tilapia fed with the NaCl-supplemented diet compared with the control group at
5 and 10 weeks, but the differences were not significant. However, dietary NaCl supplementation caused a significant decrease
in plasma nitrite levels after nitrite exposure. The dietary reduction in nitrite may be related to the increase in plasma
chloride in the 6% NaCl-supplemented fish. A direct link between the effects of dietary NaCl supplementation on methemoglobin
(MetHb) could not be established. Tilapia in this study were subjected to acute nitrite toxicity. Dietary sodium chloride
may be more effective in protecting against nitrite toxicity at lower levels of nitrite, but the conditions at which it proves
to be effective may be limited and requires further investigation. Feeding NaCl to tilapia did not affect susceptibility to
S. iniae or immune function, but nitrite exposure cause a stress-related reduction in non-specific immune function. This is the first
study to examine the effects of dietary salt on nitrite toxicity in tilapia. 相似文献
5.
Mediha Yildirim-Aksoy Chhorn Lim Menghe H Li & Phillip H Klesius 《Aquaculture Research》2008,39(11):1198-1209
This study was conducted to evaluate the effect of dietary levels of vitamins C (0, 100 and 2000 mg kg−1 ), E (0, 50 and 500 mg kg−1 ) and their interaction on the growth performance, liver contents of ascorbic acid and α-tocopherol, haematology and immune response of channel catfish, Ictalurus punctatus . Each diet was fed to catfish in triplicate aquaria to apparent satiation twice daily for 12 weeks. The results indicate that the amount of vitamin E contained in the basal diet (23.1 mg kg−1 ) was sufficient to promote good growth, feed efficiency and survival, but its supplementation was needed to maintain high haematological values and liver vitamin E. Supplementation of vitamin C (100 mg kg−1 ) to the basal diet containing 10.5 mg kg−1 was required for good growth, feed efficiency, survival and prevention of vertebral deformity and optimum haematological indices. Liver storage of ascorbic acid and α-tocopherol increased with increasing dietary levels of each vitamin. Dietary vitamin E levels had no effect on liver ascorbic acid content, but increasing dietary vitamin C increased liver α-tocopherol. Some measured immune parameters (serum protein and superoxide anion production) were enhanced by supplementation of vitamin C or E. Chemotaxis ratio and phagocytosis were not affected by treatments. 相似文献
6.
Chhorn Lim Mediha Yildirim-Aksoy Richard Shelby Menghe H. Li Phillip H. Klesius 《Fish physiology and biochemistry》2010,36(4):855-866
A study was conducted to determine the effect of increasing dietary levels of fish oil on vitamin E requirement and their
effect on growth performance, liver vitamin E status, and tissue proximate and fatty acid compositions of channel catfish.
Basal purified diets (42% protein and 3,800 kcal DE/kg) supplemented with 6, 10, and 14% menhaden fish oil were each supplemented
with 50, 100, and 200 mg vitamin E/kg (3 × 3 factorial experiment). Each diet was fed to juvenile channel catfish in three
random aquaria to apparent satiation twice daily for 12 weeks. Weight gain, feed intake, and feed efficiency ratio were not
affected by dietary levels of fish oil, vitamin E, or their interaction. Survival rate at the end of week 12 was significantly
lower for fish fed diets containing 14% fish oil, regardless of vitamin E content. Whole-body moisture significantly decreased
and lipid increased when dietary lipid levels were increased to 10 or 14%. Dietary vitamin E levels had no effect on body
proximate composition. Lipid content of liver was not influenced by dietary levels of fish oil and vitamin E or their interaction.
Hepatosomatic index significantly decreased with increasing lipid levels but was not affected by dietary levels of vitamin
E. Liver vitamin E increased with increasing dietary vitamin E but decreased with increasing fish oil levels. Fatty acid composition
of whole body and liver reflected that of dietary lipid but was not influenced by dietary levels of vitamin E. Whole-body
saturates increased, whereas MUFA decreased with increasing dietary levels of fish oil. Liver saturates were not affected
by fish oil levels, but MUFA and n-6 decreased and increased, respectively, with increasing fish oil levels. Total n-3 and
n-3 HUFA in both tissues increased with increasing fish oil levels in diets, but liver stored much higher levels of these
fatty acids. 相似文献
7.
Welker TL Lim C Yildirim-Aksoy M Klesius PH 《Journal of animal physiology and animal nutrition》2012,96(2):159-171
Juvenile channel catfish, Ictalurus punctatus, were fed diets supplemented with yeast or yeast subcomponents (YYS) as commercial preparations of β‐glucan (MacroGard® and Betagard A®), mannan oligosaccharide (Bio‐Mos® Aqua Grade), or whole‐cell Saccharomyces cerevisiae (Levucell SB20®) at the manufacturers’ recommended levels. Fish were fed experimental diets for 1 or 2 weeks prior to disease challenge (pre‐challenge feeding periods) and sampled at the end of each feeding period to measure haematological and immune parameters and to determine the effects of dietary YYS on resistance to Edwardsiella ictaluri, the causative agent of enteric septicaemia disease (ESC). Feeding of experimental diets continued for 3 weeks post‐challenge. In channel catfish fed diets supplemented with MacroGard®, Betagard A®, or Levucell SB20®, survival in the 1 week pre‐challenge feeding group and antibody titres in the 2 week feeding group were significantly higher post‐E. ictaluri challenge in relation to catfish fed with the control diet. In fish fed these same three diets, survival to ESC was significantly higher after 1 week vs. 2 weeks feeding, while the antibody response was significantly higher after 2 weeks vs. 1 week. Lysozyme activity was also higher in the 1 week feeding group, but the increased activity was unrelated to diet. Feeding YYS‐supplemented diets for a shorter duration of 1 week prior to challenge may prove beneficial in increasing resistance to ESC in channel catfish. However, we cannot discount that feeding YYS diets during the recovery period may have contributed to ‘glucan overload’ and reduced survival in the 2 week feeding group. 相似文献
8.
Thomas L. Welker Chhorn Lim Mediha Yildirim-Aksoy Richard Shelby Phillip H. Klesius 《Journal of the World Aquaculture Society》2007,38(1):24-35
Dietary supplementation of yeast or yeast subcomponents (YYS) as commercial preparations of β‐glucan (MacroGard®; Biotec‐Mackzymal, Tromsø, Norway; and Betagard A®; Aqua‐In‐Tech, Inc., Seattle, WA, USA), mannan oligosaccharide (Bio‐Mos? Aqua Grade; Alltech, Nicholasville, KY, USA), or whole‐cell Saccharomyces cerevisiae (Levucell SB20®; Lallemand Animal Nutrition, Milwaukee, WI, USA) at the manufacturer’s recommended levels was evaluated on the physiological performance of juvenile channel catfish, Ictalurus punctatus. Fish were fed YYS diets for 4 wk, followed by 2 wk of control diet. Fish were sampled at the end of each feeding period (4 and 6 wk) to measure hematological and immune parameters and growth and to determine the effects of dietary β‐glucan on resistance to Edwardsiella ictaluri infection and to low‐water stress (6 wk). Supplementation of YYS in diets did not affect growth performance, hematology, or immune function. Survival from E. ictaluri infection was from 5 to 17.5% higher in fish fed YYS diets than in the control group, but the increases were not significant. Some improvement in stress resistance was observed in YYS‐fed catfish after exposure to low‐water stress. Stress reduction in fish fed diets supplemented with yeast subcomponents has been reported previously, but thus far, no explanation has been proposed for this effect. The present study and the previously published research suggest that dietary YYS supplementation does not appear to improve resistance of channel catfish to E. ictaluri. 相似文献
9.
Craig A Shoemaker Chhorn Lim Mediha Yildirim-Aksoy Thomas L Welker & Phillip H Klesius 《Aquaculture Research》2006,37(12):1238-1245
This study determined the growth performance and acquired resistance of Nile tilapia, Oreochromis niloticus (L.) that survived Streptococcus iniae infection. Tilapia were challenged with three doses of S. iniae (8.8 × 103, 8.8 × 104 and 8.8 × 105 CFU fish?1 for low, medium and high challenges respectively). Groups of non‐injected and tryptic soy broth‐injected fish were maintained as controls. Significantly (P<0.05) higher mortality (45.0%) occurred in the high challenge treatment than in the low challenge treatment group (29.6%). The medium challenge group had mortality (36.3%) that did not differ significantly from the high or low treatment. Few fish died in the non‐injected and broth‐injected treatments (3.4% and 0.8% respectively). The tilapia that survived S. iniae infection used to assess growth performance were selected from survivors without gross clinical signs of disease. These fish were randomly stocked at a rate of 30 fish into each 57 L aquarium in triplicate and fed to apparent satiation for 8 weeks. No significant differences were detected in weight gain, feed intake, feed efficiency ratio or survival between S. iniae‐survived tilapia and the control treatments following the 8‐week growth performance trial. Following the 8‐week feeding study, tilapia were challenged with 1 × 106 CFU fish?1 of S. iniae to assess acquired immunity. Mean cumulative mortality was significantly higher (P<0.05) in the control treatments (41.7% for the non‐injected and 43.3% for the broth‐injected fish) than in the low, medium and high challenge treatments (7.4%, 3.3% and 8.3% respectively). Serum protein was significantly (P<0.05) elevated in the S. iniae‐survived tilapia that were subsequently challenged when compared with controls challenged for the first time. Agglutinating antibody titre was significantly higher in the fish in the medium and high challenge treatments, compared with the control fish challenged for the first time. The results suggest tilapia that survive S. iniae challenge without showing overt disease signs performed as well as non‐infected tilapia. Further, the S. iniae‐survived tilapia challenged following the 8‐week growth performance trial gained acquired resistance to homologous S. iniae challenge. 相似文献
10.
Haitham H. Mohammed Taylor L. Brown Benjamin H. Beck Mediha Yildirim-Aksoy Rashida M. Eljack 《Journal Of Applied Aquaculture》2013,25(3):193-209
ABSTRACTWe evaluated a Saccharomyces cerevisiae fermentation product (Diamond V Original XPC) in hybrid catfish (Ictalurus furcatus x I. punctatus) for its potential effects on growth, blood parameters, and disease resistance. The trial featured four levels of inclusion that were added to a commercial 32% protein floating catfish ration. Following six weeks of feeding, we observed marginally heightened resistance to columnaris disease and saw significant changes in the levels of immune effectors in the serum, including lysozyme, complement, and immunoglobulin. 相似文献