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排序方式: 共有7558条查询结果,搜索用时 15 毫秒
1.
为了解决马铃薯致病疫霉侵染马铃薯导致其死亡,进而造成马铃薯单位种植产量低的问题。本文从拮抗菌入手,归纳分析细菌类拮抗菌中的主要拮抗菌中芽孢杆菌、假单胞菌和粘细菌对马铃薯致病疫霉的抑制作用。同时,在总结前人研究的基础上,笔者认为在拮抗菌应用方面,可以采用多对一的新型平板对峙筛菌模式来模拟复杂条件下拮抗菌的拮抗作用,以期得到稳定的拮抗菌;并指出应从定植能力和根际微生物影响方面对拮抗菌实际应用的可行性进行研究;此外,提出未来对拮抗菌抑制马铃薯致病疫霉的探究应从表型层面的研究深入到分子层面,以期能成功从源头找寻抑制马铃薯致病疫霉的答案。 相似文献
2.
To determine whether turkey herpesvirus (HVT) impairs the aspecific and specific defense against an avian pneumovirus (APV) infection, specific-pathogen-free turkeys were inoculated at 7 days of age with HVT and 1, 5, or 7 wk later with APV. Clinical signs, APV replication, and development of antibodies against APV were evaluated. No differences were found between the birds that received both HVT and APV and those that received only APV. 相似文献
3.
古柏赤枯病病原菌的研究 总被引:2,自引:0,他引:2
经Koch's法则检验证明,AlternariatenuisNees(细链格孢霉)是北京地区古柏赤枯病的病原菌。它引起古柏针叶及嫩梢的大量死亡。2—3年生桧柏及侧柏苗经人工接种证明,该病菌有较强的致病力,它还侵染某些种类的杨树。该病潜育期为5—6天。对病菌生物学特性也作了研究。病原菌生长和孢子萌发最适温度为25℃F相对湿度在90%以上;最适pH值为6;葡萄糖、淀粉、蛋白胨、谷氨酸为该菌纯培养的优良营养源。 相似文献
4.
V Van Merris M Lenjou D Hoeben G Nijs D Van Bockstaele C Burvenich 《The Veterinary quarterly》2001,23(4):170-175
In vitro methylcellulose cultures of bovine bone marrow progenitor cells were developed. An existing technique described for bovine species was compared to a method for human tissue and further adapted during subsequent experiments. Bovine bone marrow samples were collected at the slaughterhouse, and mononuclear cells were separated by gradient centrifugation (1.077 g/ml specific density and 400 g). The use of 3% bovine leucocyte-conditioned medium, produced by stimulation of blood lymphocytes with 4 microg/ml concanavalin A and harvested on day 4 of culture, gave better results than the use of supernatant of the human bladder carcinoma 5637, which is widely used in human bone marrow cultures. However, bovine leucocyte-conditioned medium was not added to erythroid cultures because inhibitory effects were observed. Erythroid colonies were stimulated with erythropoietin, and hemin was added to enable microscopic identification. Reduced oxygen tension was necessary to induce growth of erythroid colonies. This was not necessary for myeloid cultures. In conclusion, the results of this study show that the growth of myeloid and erythroid colonies in methylcellulose-based medium requires different culture conditions, which are different from the culture conditions for human cells. 相似文献
5.
1988~1990年通过一年生苗的高生长、地径与合理密度的观察。兴安落叶松育苗容器材料和规格的研究结果表明,纸容器的苗木无论在苗高、地径上与其它容器比较都具有显著性差异,容器规格选择φ3.8×12.5cm为宜,较理想密度为910株/m~2。 相似文献
6.
Luc Van Ham Marc Vandevelde Miek Desmidt Mulenda Tshamala Luc De Schaepdrijver Daniel Mattheeuws 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1994,8(4):290-292
A central axonopathy in 2 male and 1 female Scottish Terrier puppies from 3 different but related litters is reported. Clinical signs consisting of severe whole-body tremors and ataxia were first detected at the age of 10 to 12 weeks. They worsened with activity and excitement and diminished during rest or sleep. Two dogs also had paraparesis. In 1 dog the neurological deficits progressed over several months. Neuropathological examination revealed widespread axonal changes, vacuolation, and gliosis in the white matter of the central nervous system. 相似文献
7.
8.
Kyungmin Han Inge Geurden Paul Van der Meeren Sungchul C. Bai Patrick Sorgeloos 《Journal of the World Aquaculture Society》2005,36(2):196-202
The present study examined the distribution of particle sizes in two experimental standardized lipid emulsions (distributed by ICES, International Council for the Exploration of the Sea) as a function of the preparation method (hand shaking or ultrasonic blending) and as a function of storage time. A 24-h enrichment trial compared the incorporated HUFA levels in the nauplii of Artemia franciscana .
The emulsion droplets in the 50% HUFA emulsion (ICES 50, ethyl ester based) were much smaller than in the 30% HUFA emulsion (ICES 30, triacylglycerol-based) in which 90% of the droplets had a diameter below 12.3 μm as compared to 1.3 pn in ICES 50. The blending method highly affected particle sizes. High-shear blending instead of the classical hand shaking of the emulsion of both ICES 30 and 50 reduced the mean particle size from 5.06 to 1.07 μm and from 0.38 to 0.11 μm, respectively. The particle size distributions remained stable during the 1-wk storage, suggesting the absence of agglomeration or bacterial development. The fatty acid composition of 24-h enriched Artemia reflected differences in the HUFA profile of the emulsions, but was independent of observed differences in the size of the particles. 相似文献
The emulsion droplets in the 50% HUFA emulsion (ICES 50, ethyl ester based) were much smaller than in the 30% HUFA emulsion (ICES 30, triacylglycerol-based) in which 90% of the droplets had a diameter below 12.3 μm as compared to 1.3 pn in ICES 50. The blending method highly affected particle sizes. High-shear blending instead of the classical hand shaking of the emulsion of both ICES 30 and 50 reduced the mean particle size from 5.06 to 1.07 μm and from 0.38 to 0.11 μm, respectively. The particle size distributions remained stable during the 1-wk storage, suggesting the absence of agglomeration or bacterial development. The fatty acid composition of 24-h enriched Artemia reflected differences in the HUFA profile of the emulsions, but was independent of observed differences in the size of the particles. 相似文献
9.
该文简要说明了产生砼结构开裂原因及预防方法,分析了一些施工工艺技术引起的结构开裂因素及控制措施。 相似文献
10.
Techniques for in vitro production of embryos have been developed world-wide in different species, with promising results in human and ruminants. Thousands of human IVF-babies have been born during the last 20 years and thousands of in vitro -produced calves have been born since the late 1980s. With current methods for bovine in vitro fertilization, about 30–40% of in vitro -fertilized bovine oocytes develop further to the blastocyst stage and can be used for transfer. A proper evaluation of blastocyst quality remains however, an important challenge for every researcher involved in embryology and for every clinician who wants to select the best embryos for transfer. This review attempts to summarize the different methods available for estimation of blastocyst quality with a special emphasis upon differential staining. 相似文献