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1.
Toru TACHIBANA Ken Takeshi KUSAKABE Sayuri OSAKI Takeshi KURAISHI Shosaku HATTORI Midori YOSHIZAWA Chieko KAI Yasuo KISO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(1):161-165
The New World monkey Aotus spp. (night monkeys) are expected
for use of valuable experimental animal with the close species of Saimiri
spp. (squirrel monkeys). Saimiri is known to show spontaneous
hypercortisolemia, although few reports in Aotus. We compared basic
states of blood steroid hormones and histological structure of the adrenal glands in two
monkeys. Serum cortisol and ACTH levels were statistically lower in Aotus
than Saimiri. Conversely, Aotus adrenocortical area
showed significant enlargement, especially at the zona fasciculata. Electron microscopic
observation at Aotus fasciculata cells revealed notable accumulation of
large lipid droplets and irregular shapes of the mitochondrial cristae. These results
suggest potential differences in cellular activities for steroidogenesis between
Aotus and Saimiri and experimental usefulness in
adrenocortical physiology and pathological models. 相似文献
2.
Tomowa SAKATA Ryuichi TATSUMI Michiko YAMADA Sei-Ichi SHIRATSUCHI Shinpei OKAMOTO Wataru MIZUNOYA Akihito HATTORI Yoshihide IKEUCHI 《Animal Science Journal》2006,77(5):518-525
We have shown in vitro that mechanical stretch triggers activation of quiescent satellite cells of skeletal muscle to enter the cell cycle through an intracellular cascade of events including nitric oxide (NO) synthesis that results in the release of hepatocyte growth factor (HGF) from its extracellular association and its subsequent presentation to signaling receptors. In order to explore the activation mechanism in vivo, stretch experiments were conducted in the living animal using our suspension model developed. This system used the weight of the hind portion of rats to stretch the inside muscles of the left hind limb suspended for a period of 0.5–2.0 h. At the end of the stretch period, the rats received an intraperitoneal injection of bromodeoxyuridine followed by immunocytochemistry for its incorporation as an index of satellite cell activation in vivo. Depending on the period of stretch, bromodeoxyuridine labeling was increased significantly over the contralateral unstretched leg or control muscle from untreated rats. A stretched muscle extract prepared from the 2 h stretched tissue by incubating it in PBS, showed the active form of HGF as revealed by immunoblotting and it could stimulate the activation of unstretched satellite cells. Also, administering NO synthase inhibitor L‐NAME prior to muscle stretch abolished the stretch activation of satellite cells. Therefore, the results from these experiments demonstrate that stretching muscle triggers NO synthesis and HGF release, which could activate satellite cells in vivo. 相似文献
3.
TSUTOMU NISHIDA 《Fisheries Oceanography》1992,1(2):143-152
Yellowfin stock structure in the Indian Ocean was studied by using industrial tuna longline fishery data. Three types of test variables were used to detect stock structure, i.e., CPUE, age-specific CPUE, and coefficient of variation for size. Time-series data of test variables were compiled for six sub-areas that were arranged by dividing the whole region systematically along longitude lines every 20 degrees. Then time-series data were smoothed by moving averages, and regressed by simple models. Patterns of time-series trends were graphically and statistically compared to classify homogeneous sub-area groups. Two assumptions were (a) that homogeneous stocks exist longitudinally and overlap in adjacent waters, and (b) that test variables within homogeneous sub-area groups are equally affected, and hence patterns of the time-series trends are similar. After graphical screening for significant sub-area groups, analysis of covariance was applied to test homogeneity of regression parameters representing patterns of the time-series trends. By classifying homogeneous sub-area groups, stock structures were determined at the P <0.05 and P <0.50 levels. The P<0.50 level was recognized as a useful criterion for ‘weak’ test variables since masked or vague structures at the P <0.05 level were likely cleared at this level in many cases. Results of this study and past stock structure studies were reviewed and compared. It was concluded that there are two major and two minor stocks of yellowfin tuna. The two major stocks (the western and the eastern) are located at 40o-90oE and 70o-130oE respectively. The minor stocks are the far western and the far eastern stocks (the latter possibly being a part of the Pacific stock), which are located westward of 40oE and eastward of 110oE respectively. Neighboring stocks are intermingled in adjacent waters. 相似文献
4.
5.
Yukiko ITO Ryuichi TATSUMI Jun-Ichi WAKAMATSU Takanori NISHIMURA Akihito HATTORI 《Animal Science Journal》2003,74(5):417-425
Myofibrillar proteins of vertebrate skeletal muscles are insoluble in solutions of ionic strength that approximate physiological conditions. We established a method to solubilize more than 80% of chicken breast muscle myofibrillar proteins in water for the use of meat as a source of food protein. SDS‐polyacrylamide gel electrophoretic patterns of water‐soluble myofibrillar proteins demonstrated that all identified myofibrillar proteins except connectin/titin were soluble in water. A part of α‐actinin was released from myofibrils by repeated washing with 2.5 mmol/L NaCl and 5 mmol/L L‐histidine solution, and subsequent destruction of connectin/titin in washed myofibrils by ultrasonication resulted in solubilization of a large fraction of chicken breast muscle myofibrillar proteins in water. Myofibrillar proteins of chicken leg, pork loin, beef shoulder loin, and lamb were also solubilized in water using this procedure. 相似文献
6.
Jun-ichi WAKAMATSU Juichi UEMURA Hiroko ODAGIRI Jun OKUI Nobutaka HAYASHI Shoji HIOKI Takanori NISHIMURA Akihito HATTORI 《Animal Science Journal》2009,80(2):198-205
Zinc protoporphyrin IX (ZPP) is a characteristic red pigment in meat products that are manufactured without the addition of a curing agent such as nitrate or nitrite. To examine the effects of impurities such as mineral components in sea salt on the formation of ZPP, we manufactured Parmatype dry-cured hams that were salted with refined salt or sea salt and examined the involvement of oxidation-reduction potential (ORP) in the formation of ZPP. The content of ZPP was increased drastically after 40 weeks. Microscopic observation showed strong fluorescence caused by ZPP muscle fiber after 40 weeks. Conversely, heme content varied considerably during processing. ORP increased during processing. However, there was no obvious difference between ham salted with refined salt and that salted with sea salt. Therefore, it was concluded that impurities in sea salt were not involved in the formation of ZPP. 相似文献
7.
Takayuki MIURA Yasuhiro KISHIOKA Jun-ichi WAKAMATSU Akihito HATTORI Takanori NISHIMURA 《Animal Science Journal》2010,81(1):102-107
Myostatin, a member of the TGF-β superfamily, is a negative regulator of skeletal muscle mass. We have recently demonstrated that decorin binds to myostatin in vitro , and that immobilized decorin within the collagen matrix prevents myostatin-mediated inhibition of myoblast proliferation. However, little is known about other ECM molecules that bind to myostatin and modulate its activity. Thus, in the present study, we investigated the interaction of several other ECM molecules with myostatin. We here show that fibromodulin, fibronectin and laminin bind to myostatin in the presence of Zn2+ with a dissociation constant ( KD ) of 10−10 ∼10−8 mol/L. Fibromodulin shows the highest affinity for myostatin among them. These results suggest that these ECM molecules may modulate myostatin activity like decorin does. 相似文献
8.
Masamine TAKANOSU Yuki HATTORI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2020,82(12):1769
Two Scottish Fold mixed cats are described in this report. Case 1 is a mixed Scottish Fold and Munchkin cat. Extremities of this cat resembled the Munchkin cat, while the ear pinna were folded forward like the Scottish Fold cat. Case 2 is a mixed Scottish Fold and American Curl cat. The ear pinna were curled caudally like the American Curl. Severe exostosis in the hind leg was observed in radiographs taken around one year of age in both cats. Both cats were dominant homozygous for c.1024G>T of the transient receptor potential vanilloid 4 gene, responsible for osteochondrodysplasia in the Scottish Fold cat. Cross breeding with Scottish Fold cats could produce unknown phenotypes, and should be avoided. 相似文献
9.
SHENJun-hui WANGYan KazushigeSOGAWA MakotoHATTORI LIUGuang-jie 《水稻科学》2003,11(1):57-61
ARC and ND colony were obtained by rearing the whitebacked planthopper (WBPH), Sogatella furcifera(Horvath), exclusively on ARC10239 (ARC, carrying resistance gene Wbph2) and N‘Diang Marie (ND, carrying resistance gene WbphS) till 15 generations. Taichung Native 1 (TN1) and Rathu Heenati (RHT) served as susceptible and resistant check, respectively. The results of electronic recording revealed that duration of salivation and X-waveform of the two colonies on their corresponding hosts was short while the duration of phloem ingestion was long. The amount of honeydew excreted by ARC and ND colony did not differ on their corresponding host varieties from TN1 and was much higher than that of RHT. The number of eggs laid on their host varieties and TN1 were significantly higher than that on RHT. No distinct change was observed for these two colonies in term of percentage of developed eggs. The nymph survival rate of ND colony on its selection host was 45.0%, significantly different from that on TN1(71.4%0) and RHT(21.0%), while that of ARCcolony was 68. 3%, not significantly different from that on TN1(77.5%), but much higher than that on RHT(22.6%).The nymphal development duration of these two colonies on the corresponding hosts was not different from that on TN1, but significantly shorter than that on RHT. In brief, these two colonies had almost adapted to their corresponding host varieties based on feeding and oviposition, but the nymphal survival rate of ND colony was still low. 相似文献
10.
HIROTO MURASE TSUTOMU TAMURA HIROSHI KIWADA YOSHIHIRO FUJISE HIKARU WATANABE HIROSHI OHIZUMI SHIROH YONEZAKI HIROSHI OKAMURA SHIGEYUKI KAWAHARA 《Fisheries Oceanography》2007,16(2):186-201
A study of common minke and Bryde's whales was conducted in the western North Pacific in the 2000 and 2001 summer seasons to estimate prey selection of cetaceans as this is an important parameter in ecosystem models. Whale sighting and sampling surveys and prey surveys using quantitative echosounder and mid‐water trawl were carried out concurrently in the study. Biomasses of Japanese anchovy, walleye pollock and krill, which were major prey species of common minke and Bryde's whales, were estimated using an echosounder. The results suggested that common minke whale showed prey selection for Japanese anchovy while they seemed to avoid krill in both the offshore and coastal regions and walleye pollock in the continental shelf region. Selection for shoaling pelagic fish was similar to that in the eastern North Atlantic. Bryde's whale showed selection for Japanese anchovy in August 2000 and July 2001, while it showed prey selection for krill in May and June in 2001. 相似文献