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排序方式: 共有87条查询结果,搜索用时 31 毫秒
1.
Swanson KS Grieshop CM Clapper GM Shields RG Belay T Merchen NR Fahey GC 《Journal of animal science》2001,79(4):919-926
The objective of this study was to assess fermentability by canine gut microflora to include shortchain fatty acid (SCFA) production, organic matter (OM) disappearance, and gas production of vegetable and fruit fiber sources compared to fiber standards (psyllium, citrus pectin, and Solka Floc). Fiber sources included apple pomace, carrot pomace, flaxseed, fruit blend (mixture of peach, almond, nectarine, and plum), grape pomace, pea hulls, pistachio, and tomato pomace. Substrates were fermented in vitro for 4, 12, and 24 h with fecal flora obtained from three healthy dogs. Citrus pectin had the highest OM disappearance, SCFA production, and gas production at all times of fermentation; psyllium was intermediate and Solka Floc was lowest. A wide variation in fermentability was noted among the vegetable and fruit fiber sources. Apple pomace, carrot pomace, and flaxseed had the greatest fermentability as assessed by OM disappearance. Pea hulls and tomato pomace had intermediate OM disappearances, and fruit blend, grape pomace, and pistachio were poorly fermented. Carrot pomace produced the largest amounts of gas and SCFA. Apple pomace produced high concentrations of gas but intermediate concentrations of SCFA. Pea hulls and tomato pomace produced intermediate concentrations of gas and SCFA, whereas flaxseed, fruit blend, grape pomace, and pistachio produced low amounts of these fermentation products. For all substrates collectively, OM disappearance was highly correlated with both gas production (r2 = 0.782 and 0.723 for 12- and 24-h values, respectively) and SCFA production (r2 = 0.737 and 0.738 for 12- and 24-h values, respectively). In general, OM disappearance, gas production, and SCFA production were related to the insoluble:soluble fiber ratio in the samples; as the insoluble:soluble ratio decreased (increased soluble fiber), the OM disappearance, gas production, and SCFA production increased. 相似文献
2.
Etiënne L. M. Vermeirssen Carlos Mazorra de Quero Robin J. Shields Birgitta Norberg David E. Kime Alexander P. Scott 《Aquaculture (Amsterdam, Netherlands)》2004,230(1-4):547-567
In broodstocks of Atlantic halibut, Hippoglossus hippoglossus, male and female gamete production often becomes unsynchronised towards the end of the spawning season—milt becomes very viscous and difficult to express while the females are still producing batches of good quality eggs. Gonadotrophin-releasing hormone agonist (GnRHa) has been shown to stimulate spermiation in a number of fish species. Therefore, we conducted two experiments where male halibut were implanted intramuscularly with pellets containing GnRHa. The effect of the pellets was tested at three periods: before, at the height of and at the end of spermiation. In the middle period, GnRHa was tested at two doses (5 and 25 μg/kg bodyweight). Measurements were made of milt hydration, sperm motility and fertilisation rate. Implanted males began spermiation at least 4 weeks before control males. Both doses of GnRHa increased the fluidity of the milt. This effect lasted for at least 20 days in the low dose group and for 40 days in the high dose group. When applied at the end of the season, GnRHa reversed the normal trend for the milt to become more viscous. GnRHa treatments did not affect fertilisation rates obtained with the sperm. However, towards the end of the spawning season, sperm motility was enhanced in males treated with the high dose of GnRHa (25 μg/kg) compared to controls. As described previously, plasma concentrations of the gonadal steroids, 5β-pregnane-3β,17,20β-triol 20-sulphate and 17,20-dihydroxy-4-pregnen-3-one, were significantly enhanced by GnRHa treatment. Concentrations of testosterone on the other hand decreased when spermiating males were treated with GnRHa. Our data suggest that 17,20β-dihydroxy-4-pregnen-3-one or its metabolites are involved in milt hydration, possibly through affecting ion transport. 相似文献
3.
1. Removal of large woody debris (LWD) is one of the most widely practised stream alterations, particularly in sand-bed rivers of the south-eastern USA. Selective removal of LWD has been proposed as an alternative to orthodox non-selective clearing in order to conserve ecological resources, but methods for comparing hydraulic and environmental effects of selective and non-selective removal have not been developed. Conservation of stream habitats requires quantification of LWD removal impacts on physical habitat. 2. Physical characteristics of straightened, sand-bed reaches of the South Fork Obion River in western Tennessee, USA that were rich in LWD were compared with those in similar reaches where debris had recently been removed using selective removal guidelines. 3. The mean volume of LWD per unit water volume was 0.0545 in the uncleared reaches, but nearly 60% lower (0.0225) in the cleared reach. 4. A simple technique for predicting hydraulic roughness in channels with varying amounts of LWD was developed. Hydraulic roughness, as measured by the Darcy-Weisbach friction factor, was about 400% greater in uncleared reaches at base flow but declined to a level about 35% greater than for the cleared reaches at higher flows. Predicted friction factors were within 35% of measured friction factors at higher flows. 5. Physical habitat diversity in this channelized sand-bed stream was strongly related to the density of LWD. Flow conditions in the uncleared reaches were more heterogeneous than in the cleared reach, especially at low flow. At low flow, uncleared reaches tended to be shallower, have lower velocities, slightly finer bed material, and more heterogeneous conditions overall. Shannon indices based on depth and velocity were an average of 48% higher in uncleared reaches. 6. Bed sediments underneath and immediately adjacent to LWD formations were finer and contained more organic matter than sediments distant from LWD. However, when all bed samples were considered, organic content was positively correlated with median grain size. 相似文献
4.
Along the eastern seaboard of the US, Atlantic menhaden, Brevoortia tyrannus, develop characteristic ulcerative lesions, a condition termed ulcerative mycosis. These lesions are identical to those seen across Asia in fish affected by epizootic ulcerative syndrome, a condition caused by the fungus-like oomycete Aphanomyces invadans. Young-of-the-year menhaden inhabiting estuarine environments are the primary species affected in the USA and little is known about the factors involved in the initiation of the lesions, or why menhaden are predominantly infected. Atlantic menhaden, hogchoker, Trinectus maculatus, striped killifish, Fundulus majalis, and mummichog, Fundulus heteroclitus, were inoculated with A. invadans (80 zoospores per fish) to explore species differences in infection and lesion development. All four species developed lesions. Killifish developed frank lesions similar to those observed in menhaden but the gross lesions occurred later, approximately 5-10 days after those on menhaden. Hogchoker and mummichog did not develop gross skin ulcers; rather, their lesions appeared as reddened areas under the epidermis. Mummichogs also showed evidence of significant healing with a well-developed granuloma and significant myocyte regeneration. These experiments show that species barriers as well as ecological barriers can explain some of the factors involved in the development of lesions in, and specificity of the water mould for, menhaden. 相似文献
5.
F. Douglas Shields 《水产资源保护:海洋与淡水生态系统》1995,5(2):97-108
- 1 Regions of reduced velocity adjacent to spur dikes along the Lower Mississippi River are valuable aquatic habitats. Similar zones along other large rivers have been converted to terrestrial habitats by sediment deposition.
- 2 Repetitive hydrographic surveys of 26 representative groups of dikes are examined to determine the direction and rates of change.
- 3 Since the dikes were constructed, the aquatic volume and area of associated low-velocity habitats have been reduced by 38% and 17%, respectively. Examination of time series shows that most changes occur shortly after construction, and after initial adjustment, habitat area and volume fluctuate about a condition of dynamic equilibrium.
- 4 Sedimentation rates were most rapid for dike fields constructed on the inside of bends to prevent chute development. Dike fields built to force or maintain thalweg crossings exhibited erosion rather than deposition.
6.
Medium for selective isolation of Cryptococcus neoformans 总被引:30,自引:0,他引:30
A medium has been developed that permits the selective recovery of Cryptococcus neoformans from heavily contaminated materials. It employs creatinine as a nitrogen source, diphenyl (C(6)H(5)C(6)H(5)) and chloramphenicol as mold and bacterial inhibitors, and Guizotia abyssinica seed extract as a specific color marker. The medium has proved to be effective in the direct isolation of Cryptococcus neoformans from pigeon nests and from the air. 相似文献
7.
8.
Mycotic pneumonia in mariculture-reared green sea turtles 总被引:1,自引:0,他引:1
E R Jacobson J M Gaskin R P Shields F H White 《Journal of the American Veterinary Medical Association》1979,175(9):929-933
Microbiologic examination of 29 juvenile green sea turtles with a buoyancy abnormality revealed pulmonary infection with Sporotrichium sp, Cladosporium sp, and Paecilomyces sp. Histologic examination of the lungs revealed granulomatous lesions containing branching septate hyphae. The diagnosis was mycotic pneumonia. 相似文献
9.
Rothwell JT Carson J Sherwood N Shields R Hacket K Burnett T 《Australian veterinary journal》2005,83(3):154-156
OBJECTIVE: To measure the residues of spinosad and chlorhexidine in the tissues of sheep after treatment of blowfly strike. PROCEDURE: Fourteen sheep with natural myiasis and 12 with artificial infestations of Lucilia cuprina larvae had the wool removed over their infestations and were treated with an aerosol wound dressing containing spinosad and chlorhexidine. Sheep were killed up to 14 days after treatment and residues of the chemicals measured in tissues. RESULTS: Chlorhexidine was not detected in any tissue. Residues of spinosad were highest in fat, lowest in muscle and intermediate in liver and kidney. The highest residue detected was 0.2 mg/kg spinosad in perirenal fat 7 days after generous treatment of a sheep with a large fly strike. Residues of spinosad in fat peaked 3 to 7 days after treatment and 1 to 3 days after treatment in liver and kidney. CONCLUSION: These studies present a realistic worst-case in struck sheep and at the highest dose studied, equivalent to 5.8 mg spinosad per kg body weight, the maximum residue detected of 0.2 mg/kg in peri-renal fat was 20% of the Australian maximum residue limit. Muscle, liver and kidney residues of spinosad were also below the Australian maximum residue limits at all times. 相似文献
10.
An experiment was conducted with 64 first-litter sows to evaluate the efficacy of a D2O dilution procedure for measuring in vivo body composition during the reproduction cycle. Eight gilts were each infused at breeding, 57 and 105 d postcoitum and at 5 and 25 d postpartum, with equivalent numbers of nongravid controls infused at corresponding periods except at 5 d postpartum. Results from D2O dilution were compared with body water estimates obtained from chemical analysis. An early-equilibrating D2O pool (before 15 min) was similar quantitatively to empty body (ingesta free) water in nongravid and lactating animals, but not in pregnant sows. Because of inconsistent D2O equilibration patterns in gravid sows, the early pool was considered to have equilibrated with part but not all of the water in the conceptus products. Total body D2O space measurement obtained from data following equilibration of D2O in the entire body (1 to 2 h) overestimated total body water (including gastrointestinal water) by approximately 19%. Coefficients of determination for equations relating total body D2O space to empty body and maternal body water were .96 and .88, respectively, in gestating sows and .67 and .74, respectively, for lactating sows, while coefficients of variation were below 6% in all cases. Prediction equations were developed to estimate empty and maternal body components (protein, fat and ash) from body weight and D2O space. Accuracy of protein and ash weight prediction is lowest with this procedure because it involves the composite error of estimation of the other body components. 相似文献