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排序方式: 共有954条查询结果,搜索用时 15 毫秒
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Alessandra Bertoli Anthea LoBue Luca Quattrini Stefania Sartini Beatrice Polini Sara Carpi Francesco Paolo Frontini Graziano Di Giuseppe Graziano Guella Paola Nieri Concettina La Motta 《Marine drugs》2022,20(11)
Euplotin C is a sesquiterpene of marine origin endowed with significant anti-microbial and anti-tumor properties. Despite the promising functional profile, its progress as a novel drug candidate has failed so far, due to its scarce solubility and poor stability in aqueous media, such as biological fluids. Therefore, overcoming these limits is an intriguing challenge for the scientific community. In this work, we synthesized β-cyclodextrin-based nanosponges and investigated their use as colloidal carriers for stably complex euplotin C. Results obtained proved the ability of the carrier to include the natural compound, showing remarkable values of both loading efficiency and capacity. Moreover, it also allowed us to preserve the chemical structure of the loaded compound, which was recovered unaltered once extracted from the complex. Therefore, the use of β-cyclodextrin-based nanosponges represents a viable option to vehiculate euplotin C, thus opening up its possible use as pharmacologically active compound. 相似文献
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Francisco A Macías Nuria Chinchilla Elena Arroyo Rosa M Varela José MG Molinillo David Marín 《Pest management science》2010,66(10):1137-1147
BACKGROUND: Fifteen novel derivatives of D‐DIBOA, including aromatic ring modifications and the addition of side chains in positions C‐2 and N‐4, had previously been synthesised and their phytotoxicity on standard target species (STS) evaluated. This strategy combined steric, electronic, solubility and lipophilicity requirements to achieve the maximum phytotoxic activity. An evaluation of the bioactivity of these compounds on the systems Oryza sativa–Echinochloa crus‐galli and Triticum aestivum–Avena fatua is reported here. RESULTS: All compounds showed inhibition profiles on the two species Echinochloa crus‐galli (L.) Beauv. and Avena fatua L. The most marked effects were caused by 6F‐4Pr‐D‐DIBOA, 6F‐4Val‐D‐DIBOA, 6Cl‐4Pr‐D‐DIBOA and 6Cl‐4Val‐D‐DIBOA. The IC50 values for the systems Echinochloa crus‐galli–Oryza sativa and Avena fatua–Triticum aestivum for all compounds were compared. The compound that showed the greatest selectivity for the system Echinochloa crus‐galli–Oryza sativa was 8Cl‐4Pr‐D‐DIBOA, which was 15 times more selective than the commercial herbicide propanil (Cotanil‐35). With regard to the system Avena fatua–Triticum aestivum, the compounds that showed the highest selectivities were 8Cl‐4Val‐D‐DIBOA and 6F‐4Pr‐D‐DIBOA. The results obtained for 6F‐4Pr‐D‐DIBOA are of great interest because of the high phytotoxicity to Avena fatua (IC50 = 6 µM , r2 = 0.9616). CONCLUSION: The in vitro phytotoxicity profiles and selectivities shown by the compounds described here make them candidates for higher‐level studies. 8Cl‐4Pr‐D‐DIBOA for the system Echinochloa crus‐galli‐Oryza sativa and 6F‐4Pr‐D‐DIBOA for Avena fatua‐Triticum aestivum were the most interesting compounds. Copyright © 2010 Society of Chemical Industry 相似文献
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Prevalence of resistance to 11 antimicrobials among Campylobacter coill isolated from pigs on 80 grower-finisher farms in Ontario. 下载免费PDF全文
Norma P Varela Robert Friendship Cate Dewey 《Canadian journal of veterinary research》2007,71(3):189-194
We carried out a cross-sectional study to investigate antimicrobial resistance patterns of Campylobacter coli isolated from Ontario grower-finisher pigs. From January to June 2004, 1200 samples were collected from 80 farms by obtaining a constant number (15) of fecal samples per farm. Susceptibility of the isolates to 11 antimicrobial drugs was determined by the agar-dilution technique. The overall prevalence of resistance to 1 or more antimicrobials among the isolates was 99.2%. High levels of resistance were observed for azithromycin, clindamycin, erythromycin, streptomycin, and tetracycline: 91.7%, 82.5%, 81.4%, 70.7%, and 63.7%, respectively. For sulfamethoxazole, ampicillin, and nalidixic acid, resistance was observed in 40.3%, 26.6%, and 22.7% of the isolates, respectively. Although at very low levels, resistance was observed for ciprofloxacin (a fluoroquinolone), chloramphenicol, and gentamicin: in 2.4%, 1.7%, and 0.2%, respectively. Many of the isolates (29.7%) were resistant to 5 antimicrobials, the most common being azithromycin, clindamycin, erythromycin, streptomycin, and tetracycline. Isolates from the same farm showed at least 5 patterns of resistance. Results from this study indicate high levels of resistance to the antimicrobial drugs most commonly used in the Canadian swine industry (macrolides, lincosamides, and tetracyclines) among C. coli isolated from grower-finisher pigs in Ontario. Macrolides and fluoroquinolones are the drugs most commonly used to treat severe human campylobacteriosis. Fortunately, at present, there is little resistance to fluoroquinolones among C. coli from pigs in Ontario. 相似文献
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Clinical comparison of two regimens of lidocaine infusion in horses undergoing laparotomy for colic 下载免费PDF全文
Sara Nannarone Alessia Cenani Rodolfo Gialletti Marco Pepe 《Veterinary anaesthesia and analgesia》2015,42(2):150-156
ObjectiveTo compare, in horses undergoing laparotomy for colic, the effects of administering or not administering a loading intravenous (IV) bolus of lidocaine prior to its constant rate infusion (CRI). Effects investigated during isoflurane anaesthesia were end-tidal isoflurane concentration (Fe’ISO), cardiovascular function, anaesthetic stability and the quality of recovery.Study designProspective, randomized clinical study.AnimalsThirty-six client-owned horses.MethodsHorses were assigned randomly to receive lidocaine as a CRI (50 μg kg−1 minute−1) either preceded (LB) or not preceded (L) by a loading dose (1.5 mg kg−1 IV over 15 minutes). Lidocaine infusion (LInf) was started (T0) within 20 minutes after induction of general anaesthesia and discontinued approximately 30 minutes before the end of surgery. Anaesthetic depth, Fe’ISO, intra-operative physiological parameters and quality of recovery were assessed or measured. Data were analysed using one-way anova, t-test, Fisher test, Wilcoxon and Kruskal–Wallis tests as appropriate (p < 0.05).ResultsMean ± SD Fe’ISO was 1.21 ± 0.08% in group LB and 1.23 ± 0.06% in group L. Heart rate was significantly higher in group L than in group LB at times T5-T15, T25, T35 and T95. No difference was found between groups in other measured physiological values, nor in any measure taken to improve these parameters. Recovery phase was comparable and satisfactory in all but one full term pregnant horse in group L which fractured a femur during recovery.ConclusionPreloading with a lidocaine bolus prior to a CRI of lidocaine did not influence isoflurane requirements, cardiopulmonary effects (other than a reduction in heart rate at some time points) or recovery compared to no preloading bolus.Clinical relevanceA loading dose of lidocaine prior to CRI does not confer any advantage in horses undergoing laparotomy for colic. 相似文献
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Paralytic shellfish poisoning (PSP), due to saxitoxin and related compounds, typically results from the consumption of filter-feeding molluscan shellfish that concentrate toxins from marine dinoflagellates. In addition to these microalgal sources, saxitoxin and related compounds, referred to in this review as STXs, are also produced in freshwater cyanobacteria and have been associated with calcareous red macroalgae. STXs are transferred and bioaccumulate throughout aquatic food webs, and can be vectored to terrestrial biota, including humans. Fisheries closures and human intoxications due to STXs have been documented in several non-traditional (i.e. non-filter-feeding) vectors. These include, but are not limited to, marine gastropods, both carnivorous and grazing, crustacea, and fish that acquire STXs through toxin transfer. Often due to spatial, temporal, or a species disconnection from the primary source of STXs (bloom forming dinoflagellates), monitoring and management of such non-traditional PSP vectors has been challenging. A brief literature review is provided for filter feeding (traditional) and non-filter feeding (non-traditional) vectors of STXs with specific reference to human effects. We include several case studies pertaining to management actions to prevent PSP, as well as food poisoning incidents from STX(s) accumulation in non-traditional PSP vectors. 相似文献
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Landuse can alter soil organic carbon (SOC) fractions by affecting carbon inflows and outflows. This study evaluated changes in SOC fractions in response to different landuses under variable rainfalls. We compared cropland, grassland and forest soils in high rainfall (Islamabad ~1142 mm) and low rainfall (Chakwal ~667 mm) areas of Pothwar dryland, Pakistan. Forest soils in both rainfall areas had highest SOC (11.32 g kg?1), particulate organic carbon (POC, 1.70 g kg?1), mineral-associated organic carbon (MOC, 7.17 g kg?1) and aggregate-associated organic carbon (AOC, 7.86 g kg?1). However, in rangeland and cropland soils, these varied with rainfall. Under high rainfall, SOC and MOC were 12% and 17% higher in rangeland than in cropland while POC and AOC were equal. Under low rainfall, SOC and MOC were higher in rangeland than in cropland by 7.21 and 1.79 g kg?1 at 0–15 cm and equal at 15–30 cm depth. POC and AOC were higher in rangeland than in cropland, in both depths. Averagely, SOC, POC, MOC and AOC were 26%, 68%, 76% and 30% higher in high rainfall than in low rainfall soils. Sensitivity of SOC fractions to landuses observed under different rainfalls could provide useful information for soil management in subtropical drylands. 相似文献
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OBJECTIVE: To evaluate biochemical analytes in vitreous humor collected after death in West Indian manatees (Trichechus manatus latirostris). DESIGN: Prospective study. ANIMALS: 73 West Indian manatees of various ages and both sexes. PROCEDURE: The condition of manatee carcasses submitted for routine postmortem examination was categorized as fresh, moderately autolyzed, or severely autolyzed. The eyes were removed, frozen for shipping, and thawed on arrival at the laboratory. Vitreous humor was extracted, and various biochemical analytes were measured. Values for each analyte were compared with published reference ranges for corresponding biochemical analytes in serum. Values were also compared among the carcass condition groups. RESULTS: Creatinine and urea nitrogen concentrations in vitreous humor were significantly higher in severely autolyzed carcasses than in fresh carcasses. Potassium concentrations in vitreous humor were significantly higher in moderately autolyzed carcasses than in fresh carcasses but were highly variable in severely autolyzed carcasses. These data were consistent with those reported in other species. CONCLUSIONS AND CLINICAL RELEVANCE: Results indicate that measurement of biochemical analytes in vitreous humor of manatees is feasible and has potential for assisting in the postmortem diagnosis of certain metabolic, renal, and nutritional diseases; determining severity of autolysis at time of necropsy; and determining the time of occurrence of human-induced trauma. 相似文献