Detection of soluble antigens of Erwinia salicis in leaves of Salix alba by Enzyme-linked Immunosorbent Assay

Soluble antigens of Erwinia salicis are transported through the xylem vessels to the leaves of Salix species. These antigens can be detected by the use of the Enzyme-linked Immunosorbent Assay (ELISA) in willow trees in the absence of bacterial cells and are also produced in pure cultures. In this way populations of E. salicis occurring in stem or branches can be traced in the leaves. The possibilities for more general application in the detection of vascular parasites is discussed.     

Schisandrin B protects against solar irradiation-induced oxidative injury in BJ human fibroblasts

The effects of schisandrin B (Sch B) and its analogs on solar irradiation-induced oxidative injury were examined in BJ human fibroblasts. Sch B and schisandrin C (Sch C) increased cellular reduced glutathione (GSH) level and protected against solar irradiation-induced oxidative injury. The photoprotection was paralleled by decreases in the elastases-type protease activity and matrix-metalloproteinases-1 expression in solar-irradiated fibroblasts. The cytochrome P-450-mediated metabolism of Sch B or Sch C caused ROS production. The results suggest that by virtue of its pro-oxidant action and the subsequent glutathione antioxidant response, Sch B or Sch C may offer the prospect of preventing skin photo-aging.     

Research on poplar canker (Xanthomonas populi subsp. populi) in the Netherlands. Part III

A new inoculation method for testing the resistance of poplars to bacterial canker caused by Xanthomonas populi subsp. populi (Ridé) Ridé & Ridé is discussed. The procedure is based on a more natural infection method and involves spraying plants in spring with bacterial suspensions.     

Comparison of two methods to measure the susceptibility of poplar clones to Xanthomonas populi

The conventional method of testing susceptibility to Xanthomonas populi by means of leaf-scar inoculation in September is compared with stipule-scar inoculation in spring. A method for evaluating the resulting disease expression is presented.     

Domestication of plants for sustainable agriculture in drylands: Experience from the Negev Desert

With globalization in the last century, introduction of exotic plant species for commercial use has become more accessible. Such attempts may involve extreme land changes. We stress that domestication of native species should be preferred to the introduction of exotic species. We took the initial steps in domesticating several species by examining commercial uses and studying aspects of plant physiology. The following desert plants were considered: Bassia indica, for salt phytoremediation and for livestock feed; Commiphora gileadensis, as an agent against cancer cells; Artemisia sieberi and A. judaica, as plants with allopathic traits; Ficus palmate, as a stand for fig plantation; Balanites aegyptiaca, as a medicinal plant and for other uses; Portulaca oleracea and Scorzonera judaica, as food crops with added values; and Pistacia atlantica, as rootstock for P. vera.     

Economics of milkfish (Chanos chanos) production in Hawaii*

Abstract

Economic evaluations of commercial milkfish (Chanos chanos) growout systems were made based on current Hawaiian fishpond, pond and tank culture techniques in Hawaii. Based on the observed practices of milkfish culture as a secondary or tertiary crop, capital costs and several operating costs were pro‐rated to accurately depict current farm practices. For a target harvest weight of 0.80 lb, the study estimates the total production cost for milkfish production at $3.31/lb for the pond system, $3.81/lb for the tank system and $1.84/lb for the Hawaiian fishpond system. At a sale price of $3.00/lb and seed cost of $0.25/fingerling, only the Hawaiian fishpond system is profitable under a 20‐year project life. When considering variable costs alone, the production cost is $1.78/lb for the pond system, $2.33/lb for the tank system and $1.53 for the Hawaiian fishpond system, yielding returns on variable costs of 67%, 30% and 59% respectively. Analyses of profit sensitivity to sale price, production yield, labor, feed and stocking indicate that sale price, as expected, has the largest impact on profitability, followed by feed. The results of this study are consistent with the Hawaii farmers’ view of milkfish as a secondary specie to core production based upon current market conditions and input requirements.     

Schisandrin B co-treatment ameliorates the impairment on mitochondrial antioxidant status in various tissues of long-term ethanol treated rats

The effect of schisandrin B (Sch B) on long-term ethanol-induced oxidative stress in various rat tissues was investigated. Long-term ethanol treatment increased reactive oxygen metabolites (ROM) level in plasma. The ethanol-induced oxidative stress was assessed by mitochondrial glutathione and α-tocopherol levels, antioxidant enzyme activities, malondialdehyde (mtMDA) production and heat shock protein (Hsp) 25/70 levels. Liver was most susceptible to oxidative stress with a significant increase in mtMDA production. Long-term Sch B treatment enhanced mitochondrial antioxidant status in a tissue non-specific manner. Sch B co-treatment ameliorated the alterations in plasma ROM levels, mtMDA production and Hsp 25/70 expression in rat tissues.     

Genetic differentiation between fake abalone and genuine Haliotis species using the forensically informative nucleotide sequencing (FINS) method

Abalones ( Haliotis species) are a popular delicacy and commonly preserved in dried form either whole or in slices or small pieces for consumption in Asian countries. Driven by the huge profit from trading abalones, dishonest traders may substitute other molluscan species for processed abalone, of which the morphological characteristics are frequently lost in the processed form. For protection of consumer rights and law enforcement against fraud, there is a need for an effective methodology to differentiate between fake and genuine abalone. This paper describes a method (validated according to the international forensic guidelines provided by SWGDAM) for the identification of fake abalone species using forensically informative nucleotide sequence (FINS) analysis. A study of the local market revealed that many claimed "abalone slice" samples on sale are not genuine. The fake abalone samples were found to be either volutids of the genus Cymbium (93%) or the muricid Concholepas concholepas (7%). This is the first report of Cymbium species being used for the preparation and sale as "abalone" in dried sliced form in Hong Kong.     

Impact of seawater intrusion control on the environment,land use and household incomes in a coastal area

Agricultural production in the coastal wetlands of Asia is often hindered by salinity intrusion caused by tidal fluctuation. This paper reports changes in environmental and socio-economic conditions that followed the phased construction and operation of sluices for controlling seawater intrusion from 1994 –2000 in a coastal area of the Mekong River Delta, Vietnam. Canal water salinity decreased rapidly upstream of sluices, allowing rice cropping intensification and increased rice production in the eastern part of the study area. However, the livelihoods of farmers in the western part were adversely affected due to cessation of supply of brackish water that was needed for brackish-water shrimp farming, while the acid sulphate soils present there posed problems for rice cultivation. The poor farmers and landless people suffered more because the fishery resource that they depended on declined sharply due to reduced salinity and increased acidity in the canal water. The findings confirmed that the environment and resource use in the coastal lands are very sensitive to external intervention. A clear understanding of the socio-economic and environmental impacts of salinity control measures in coastal areas can help planning to enhance farmers' incomes while minimizing negative environmental impacts. Land-use policy formulation, planning and management should adopt a more holistic approach, taking into account the interests of all resource users, especially the poor, instead of focusing on any particular sector.