In vivo and in vitro anti-inflammatory activity of neorogioltriol, a new diterpene extracted from the red algae Laurencia glandulifera

Neorogioltriol is a tricyclic brominated diterpenoid isolated from the organic extract of the red algae Laurencia glandulifera. In the present study, the anti-inflammatory effects of neorogioltriol were evaluated both in vivo using carrageenan-induced paw edema and in vitro on lipopolysaccharide (LPS)-treated Raw264.7 macrophages. The in vivo study demonstrated that the administration of 1 mg/kg of neorogioltriol resulted in the significant reduction of carregeenan-induced rat edema. In vitro, our results show that neorogioltriol treatment decreased the luciferase activity in LPS-stimulated Raw264.7 cells, stably transfected with the NF-κB-dependent luciferase reporter. This effect on NF-κB activation is not mediated through MAPK pathways. The inhibition of NF-κB activity correlates with decreased levels of LPS-induced tumor necrosis factor-alpha (TNFα) present in neorogioltriol treated supernatant cell culture. Further analyses indicated that this product also significantly inhibited the release of nitric oxide and the expression of cyclooxygenase-2 (COX-2) in LPS-stimulated Raw264.7 cells. These latter effects could only be observed for neorogioltriol concentrations below 62.5 μM. To our knowledge, this is the first report describing a molecule derived from Laurencia glandulifera with anti-inflammatory activity both in vivo and in vitro. The effect demonstrated in vitro may be explained by the inhibition of the LPS-induced NF-κB activation and TNFα production. NO release and COX-2 expression may reinforce this effect.     

The role of NGOs in improving social forestry practice: Do they promote livelihood, sustainability and optimal land use in Bangladesh?

The encroachment rate in forests in Bangladesh is high and increasing — accelerated by rural poverty and the demand for dwelling space and forest products — causing environmental degradation as well as loss of forest cover and productivity. The forests are managed by the Forest Department, although a substantial area of marginal land belongs to other semi-public agencies including Roads and Highways and the Water Development Board. This marginal land has been left unused or underutilized. In contrast, nongovernmental organizations have an appropriate accessibility and technology disseminating ability to utilize this land in reducing poverty and enhancing rural livelihood, and have been highly active and successful in rehabilitating encroached forests. NGOs have added a new dimension to forest management, which has ensured community participation and protection of the forests, both planted and natural. This study evaluates the social forestry activities of four large NGOs, namely BRAC, Proshika, Caritas and CARE-Bangladesh, as well as national social forestry activities. By adopting a common partnership between public and private authority, property right conflicts have been resolved and rural livelihoods enhanced, and scope has been created for utilizing marginal land. The NGO partnership has been effective in reducing poverty and improving livelihoods. As an outcome of this common partnership, 33,472 km of roadside planting and 53,430 ha of reforestation activities have been carried out during the last two decades.     

Socio-economic factors affecting the income of small-scale agroforestry farms in hill country areas in Yemen: A comparison of OLS and WLS determinants

Yemen is a less-developed country in the Arabian Peninsula, with only 3% arable land. An agroforestry land-use system has been practiced traditionally by small-scale farmers, but is associated with low productivity and income. A study has been undertaken to determine the socio-economic attributes of farmers that influence the financial performance of agroforestry and non-agroforestry farms in the Bura’a Mountain region. A survey was conducted of 150 farmers involved in both agroforestry and non-agroforestry. Both OLS and WLS regression were applied, and coefficients compared in terms of consistency and goodness of fit. Incomes of farmers were found to be influenced by education, area of land, livestock holding, family size, and whether coffee is grown, but not farmer’s age. The WLS method produced efficient and consistent results, whereas OLS regression was affected by the heteroscedasticity. The findings of the study indicate that the farmers of the study area are in need of financial and technical support from government to increase their income. Infrastructural development and public intervention in developing farmers’ technical know-how could enhance production and ensure the optimum use of land as well as soil and water conservation.     

Genetic structure of Tunisian natural carob tree (Ceratonia siliqua L.) populations inferred from RAPD markers

? Seven RAPD markers were used to assess the genetic diversity and structure of ten Tunisian natural Ceratonia siliqua L. populations from different geographic and bioclimatic zones.

? The species maintain a high diversity within population as estimated by the percentage of polymorphic loci and Shannon’s index (P% = 76.31, $\bar H'_{pop} = 0.569$ ). The range of variation between populations was large. Populations from the upper semi-arid bioclimates, with more continuous distribution area showed the highest level of variation.

? A high genetic differentiation among populations (Φ_ST = 0.250 and $\bar G_{ST} = 0.347$ ), as a result of population isolation was revealed. Nevertheless, the genetic structure is in accordance with bioclimate indicating that ecological factors also should influence differentiation. Populations from the sub-humid, upper semi-arid and mean semi-arid zones clustered together and were distinct from those of the lower semi-arid ones.

? Conservation strategy should be made according to the level of polymorphism within population and bioclimate.

     

Feed intake, nutrient digestibility and ruminal fermentation activities in sheep-fed peanut hulls treated with Trichoderma viride or urea

This study aimed to assess impacts of fungal treatment on the nutritional value of peanut hulls (PH) or urea at the rate of 5 kg/100 g of PH. Fermented sugar beet pulp inoculated with Trichoderma viride was supplemented to PH at rates of 5.0, 10.0 and 15.0 g/100 g air dry of PH and mixed well before aerobic incubation for 21 days. Organic matter (OM) content of PH declined with increased levels of fermented sugar beet pulp inoculums, while crude protein (CP), ether extract (EE), and ash increased. Fiber contents were decreased with both treatments of fermented sugar beet pulp and urea. Total N of PH increased with urea treatment, which reduced the true protein N to total protein N ratio. In sacco degradabilities of dry matter (DM), OM, and CP with urea treatment increased compared with fungal treatment. The DM intake of peanut hulls treated with fungus (PHF) was higher (P?<?0.05) than with peanut hulls treated with urea (PHU). Digestibility of OM, CP, neutral detergent fiber, and non-fiber carbohydrate by native breed Ossimi sheep with PH were higher (P?<?0.05) than with PH or urea treated PH. The intakes, losses, and balance of N increased (P?<?0.01) with PHF versus PH feeding. Feeding PHF increased (P?<?0.01) ruminal concentrations of NH₃-N, acetic acid, butyric acid, and the acetic to propionic acid ratio. Bacterial and protozoal counts increased (P?<?0.05) with feeding PHF or PHU versus PH. Overall, this fungal treatment of peanut hulls created a higher nutritive value feed for ruminants.     

Conidia of black aspergilli as new biological adsorbents for ochratoxin A in grape juices and musts

Biological removal of ochratoxin A (OTA) by living and heat-treated dead conidia of black Aspergillus isolates representing the species Aspergillus niger, Aspergillus carbonarius, and Aspergillus japonicus in synthetic and natural grape juices was found to be a two-stage phenomenon. Several lines of evidence suggest that the first observed stage was passive, metabolism was not required, and OTA adsorption on conidia of black aspergilli could be involved. This removal was fast, without delay just after conidial inoculation both in synthetic and natural grape juices. Moreover, even nonviable, heat-treated conidia were capable of removing OTA. Finally, no OTA degradation products were detected. In the second observed stage, removal of OTA was linked to degradation by live conidia only. Ochratoxin alpha, a degradation product of OTA, was detected in the medium after incubation for 30 and 14 h for biseriate (A. niger and A. carbonarius) and uniseriate (A. japonicus) black aspergilli, respectively, when well-developed mycelium appeared. Comparisons between the three black Aspergillus isolates tested showed that A. carbonarius detoxified grape juice most effectively. However, this species often produces OTA. A. niger and A. japonicus isolates were also effective and because those species are not systematically OTA producers, they could be interesting for further OTA detoxification processes in grape juices and musts.     

Genetic diversity in Tunisian Crataegus azarolus L. var. aronia L. populations assessed using RAPD markers

? The genetic diversity of nine wild Tunisian Crataegus azarolus var. aronia L. populations from different bioclimates was assessed using RAPD markers.

? Eight selected primers generated a total of 105 bands, 81 of which were polymorphic. Shannon’s index (H′) ranged from 0.222 to 0.278 according to a population with an average of 0.245. The genetic variation within the species (H _SP = 0.423) was relatively low. A high differentiation (G _ST = 0.421) among populations coupled with a low level of gene flow (N _m = 0.472) were observed. The analysis of molecular variance (AMOVA) revealed also significant differentiation among populations (Φ_ST = 0.371), even at a low scale space. The majority of variation occurred within populations (63.31%). The Mantel test performed on genetic (Φ_ST) and geographic distance matrices among population pairs did not reveal an isolation by distance.

? Interpretation of Neighbour-joining tree based on Nei’s and Li’s genetic distance among individuals showed distinct population groupings. The UPGMA dendrogram based on Φ_ST values revealed two population sub-clusters, each including populations from different bioclimates and/or geographic regions.

? The low level of genetic diversity and the high genetic structure of populations resulted from genetic drift caused both by habitat fragmentation and the low size of populations.

? The high differentiation among populations and the similar low level of diversity within populations suggest that in situ conservation should interest all populations. The ex situ conservation should be based on the collection of seeds rather within than among populations because of the maximum of variation was revealed within populations.

     

The effect of ZnSO4 and KNO3 on quantitative and qualitative properties of Punica granatum L.

Abstract

We investigated the effect of foliar application of potassium nitrate (KNO₃) and zinc sulfate (ZnSO₄) on the fruit quality of pomegranate (Punica granatum L. cv. Malas-E–Torsh in Saveh). Potassium was applied as KNO₃ at concentrations of 0%, 0.25%, and 0.5% and zinc was applied as ZnSO₄ at concentrations of 0%, 0.5%, and 1% in a completely randomized block design. To determine the effect of the treatments on fruit quality, we measured fruit weight, peel thickness, aril diameter, phenol content of the juice, pH, total soluble solids (TSS), titratable acidity (TA), and antioxidant capacity. Foliar application of KNO₃ significantly increased fruit weight, 100 aril weight, peel weight, peel thickness, TTS, and antioxidant capacity. In contrast, pH, TA, TSS/TA, and phenol content of the juice were not influenced by foliar application of KNO₃. ZnSO₄ significantly increased fruit weight, aril diameter, and phenol content of the juice. KNO₃ 1% and ZnSO₄ 0.5% were identified as the most appropriate treatments in improving quality and quantity of pomegranate. The fatty acid profile of the pomegranate oil was primarily composed of punicic acid, linoleic acid, oleic acid, palmitic acid, and stearic acid.