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1.
Hiroto Miura Takuya Hashimoto Yukiko Kawanishi Hiroki Kawauchi Ryo Inoue Noriaki Shoji Kunihiko Saito Mario Sekiya Yosuke Saito Jumpei Yasuda Chiemi Yonezawa Tetsushiro Endo Hirotaka Kasuya Yutaka Suzuki Yasuo Kobayashi Satoshi Koike 《Animal Science Journal》2021,92(1):e13601
The rumen microbiota comprises a vast range of bacterial taxa, which may affect the production of high-quality meat in Japanese Black cattle. The aim of this study was to identify core rumen microbiota in rumen fluid samples collected from 74 Japanese Black cattle raised under different dietary conditions using 16S rRNA gene amplicon sequencing. In the rumen of fattening Japanese Black cattle, 10 bacterial taxa, showing >1% average relative abundance and >95% prevalence, irrespective of the dietary conditions and the fattening periods, were identified as the core rumen bacterial taxa, which accounted for approximately 80% of the rumen microbiota in Japanese Black cattle. Additionally, population dynamics of the core rumen bacterial taxa revealed two distinct patterns: Prevotella spp. and unclassified Bacteroidales decreased in the mid-fattening period, whereas unclassified Clostridiales, unclassified Ruminococcaceae, Ruminococcus spp., and unclassified Christensenellaceae increased during the same period. Therefore, the present study reports the wide distribution of the core rumen bacterial taxa in Japanese Black cattle, and the complementary nature of the population dynamics of these core taxa, which may ensure stable rumen fermentation during the fattening period. 相似文献
2.
Umehashi M Imamura T Akiyama S Kimachi K Tokiyoshi S Mikami T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(11):1017-1021
In order to confirm the in vivo effectiveness of anti- feline herpesvirus type 1 (FHV-1) mouse-cat chimeric antibody (FJH2), and anti-feline calicivirus (FCV) mouse-cat chimeric antibody (F1D7), cats that had been experimentally infected with FHV-1 or FCV were administered intravenously with the chimeric antibodies, and observed for clinical manifestations. The symptoms due to FHV-1 or FCV infection in the cats administered FJH2 or F1D7 were obviously decreased when compared with those of the non-administered control cats. From these results, it was confirmed that both FJH2 and F1D7 were effective at reducing the appearance of symptoms due to FHV-1 and FCV infection, respectively. 相似文献
3.
Yurimoto H Abe K Abe M Ebihara M Fujimura A Hashiguchi M Hashizume K Ireland TR Itoh S Katayama J Kato C Kawaguchi J Kawasaki N Kitajima F Kobayashi S Meike T Mukai T Nagao K Nakamura T Naraoka H Noguchi T Okazaki R Park C Sakamoto N Seto Y Takei M Tsuchiyama A Uesugi M Wakaki S Yada T Yamamoto K Yoshikawa M Zolensky ME 《Science (New York, N.Y.)》2011,333(6046):1116-1119
Meteorite studies suggest that each solar system object has a unique oxygen isotopic composition. Chondrites, the most primitive of meteorites, have been believed to be derived from asteroids, but oxygen isotopic compositions of asteroids themselves have not been established. We measured, using secondary ion mass spectrometry, oxygen isotopic compositions of rock particles from asteroid 25143 Itokawa returned by the Hayabusa spacecraft. Compositions of the particles are depleted in (16)O relative to terrestrial materials and indicate that Itokawa, an S-type asteroid, is one of the sources of the LL or L group of equilibrated ordinary chondrites. This is a direct oxygen-isotope link between chondrites and their parent asteroid. 相似文献
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Mitsuhiro Nakaya Taizo Morioka Kyouhei Fukunaga Naoto Murakami Takashi Ichikawa Sachio Sekiya Satoshi Suyama 《Fisheries Science》2010,76(1):45-53
This study details the growth and maturation processes of Pacific saury Cololabis saira from eggs to first spawning under laboratory conditions. They were reared at 20°C, and fed almost to satiation every day. There was no significant difference in the knob length (KnL) between males and females, and so data for both sexes were combined in the following Gompertz growth formula: KnL t = 277.1 exp[?exp(?0.015 (t ? 83.8))]. The first spawning was observed 243 days after hatching (DAH). At the beginning of spawning, when the mean KnL was about 250 mm, only several hundred eggs were spawned at most. The spawning continued, and the number of spawned eggs increased notably after 260 DAH. Correlation and stepwise multiple regression analysis of the gonad somatic index (GSI) of Pacific saury versus KnL, CF, and DAH revealed that only DAH (R = 0.88 and 0.72 for males and females, respectively) was significantly correlated with GSI of Pacific saury (P < 0.001). This result suggests that DAH is one of the most influential factors for maturation in this species. 相似文献
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Abstract Nitrogen contents, nodule numbers, and nodule dry weights of 6-week-oId Sesbania rostrata plants grown in sand culture with only root nodules, only stem nodules or with both were compared and the root nodules were found to contribute to nitrogen acquisition more significantly than the stem nodules. Similar findings were obtained in 15N2-fixing experiments. An 8-week-old plant with both stem and root nodules fixed 1.50 mg nitrogen in a 12 h light period, while the fixation decreased to 1.15 mg nitrogen after the removal of the stem nodules, suggesting that root nodules played major role in nitrogen fixation. However, acetylene-reducing activities per nodule dry weight were higher in the stem nodules. Under flooding conditions, the aerenchyma tissues contributed to about 40% of N2 transport to root nodules, and 60% was supplied through stem. 相似文献
8.
Water penetration into rice grains during soaking observed by gradient echo magnetic resonance imaging 总被引:3,自引:2,他引:3
Akemi K. Horigane Hitoshi Takahashi Sachio Maruyama Kenichi Ohtsubo Mitsuru Yoshida 《Journal of Cereal Science》2006,44(3):307-316
The water penetration into a rice grain during soaking was monitored by three-dimensional gradient echo magnetic resonance imaging, which has the advantage of a short measurement time and a sufficient sensitivity for low-moisture samples. The water penetration route was compared between the milled and brown rice grains of two japonica cultivars. In normal milled rice grains of Koshihikari, water first penetrated the ventral side surface and the embryo attachment site of the endosperm, then migrated along the central line and transverse cracks, and finally diffused to all parts of the endosperm. In milled rice grains of Yamadanishiki with a white core, water quickly infiltrated into the cracks or chalky parts on the dorsiventral line and then diffused to the lateral side. In brown rice grains of both cultivars water penetrated extremely slowly and did not infiltrate directly into transverse cracks or the white core due to inhibition by the pericarp and seed coat. The central part of every grain allowed more water to penetrate. The route, pattern and speed of water penetration were determined by the morphological structure, crack formation and hardness distribution associated with the packing of the starch granules in the grain. 相似文献
9.
Buffoni L Martínez-Moreno FJ Zafra R Mendes RE Pérez-Écija A Sekiya M Mulcahy G Pérez J Martínez-Moreno A 《Veterinary parasitology》2012,185(2-4):315-321
The humoral immune response was analysed in goats immunised with FhCL1, FhPrx, Sm14, and experimentally challenged with Fasciola hepatica. All immunised animals developed significant levels of anti-fluke specific antibodies and those immunised with FhCL1 showed the highest antibody titre. After experimental infection, an increase in the antibody level was detected only in goats immunised with FhCL1. In the adjuvant-control animals, the experimental challenge induced significant production of specific antibodies against FhCL1, FhPrx and Sm14. While liver fluke specific humoral responses were seen in all groups, no significant protection in any of the vaccinated groups was found. 相似文献
10.
Teruo Nonomura Hiromi Tajima Yuko Kitagawa Naoko Sekiya Kayoko Shitomi Mami Tanaka Kazuhiko Maeda Yoshinori Matsuda Hideyoshi Toyoda 《Journal of General Plant Pathology》2003,69(1):45-48
Green fluorescent protein (GFP)-marked Fusarium oxysporum f. sp. melonis and nonmarked F. oxysporum f. sp. fragariae were stained with neutral red. The neutral red stained vacuoles of the fungi without disturbing GFP fluorescence in the cytoplasm.
GFP-marked fungi showed fluorescent hyphae with dark-stained vacuoles, whereas nonmarked fungi were detected as nonfluorescent
hyphae with dark-dotted vacuoles. Root colonization by these two fungi was monitored using this method. Microconidia attached
similarly to the root surface and elongated vegetative hyphae. Only the pathogenic fungi invaded, causing necrosis at the
inoculation site. Thus, the present method enabled us to track simultaneously the various formae speciales of F. oxysporum colonizing the root surface.
Received: March 25, 2002 / Accepted: September 27, 2002 相似文献