全文获取类型
收费全文 | 762篇 |
免费 | 50篇 |
专业分类
林业 | 26篇 |
农学 | 16篇 |
52篇 | |
综合类 | 97篇 |
农作物 | 28篇 |
水产渔业 | 61篇 |
畜牧兽医 | 484篇 |
园艺 | 12篇 |
植物保护 | 36篇 |
出版年
2023年 | 4篇 |
2021年 | 7篇 |
2020年 | 17篇 |
2019年 | 5篇 |
2018年 | 19篇 |
2017年 | 15篇 |
2016年 | 9篇 |
2015年 | 16篇 |
2014年 | 28篇 |
2013年 | 30篇 |
2012年 | 29篇 |
2011年 | 31篇 |
2010年 | 19篇 |
2009年 | 43篇 |
2008年 | 22篇 |
2007年 | 30篇 |
2006年 | 20篇 |
2005年 | 17篇 |
2004年 | 21篇 |
2003年 | 26篇 |
2002年 | 37篇 |
2001年 | 26篇 |
2000年 | 17篇 |
1999年 | 15篇 |
1998年 | 24篇 |
1997年 | 14篇 |
1996年 | 13篇 |
1995年 | 13篇 |
1994年 | 6篇 |
1993年 | 11篇 |
1992年 | 20篇 |
1991年 | 15篇 |
1990年 | 22篇 |
1989年 | 24篇 |
1988年 | 8篇 |
1987年 | 10篇 |
1986年 | 10篇 |
1985年 | 7篇 |
1984年 | 8篇 |
1983年 | 6篇 |
1982年 | 4篇 |
1979年 | 5篇 |
1978年 | 4篇 |
1976年 | 5篇 |
1974年 | 6篇 |
1971年 | 7篇 |
1970年 | 8篇 |
1896年 | 5篇 |
1888年 | 3篇 |
1884年 | 4篇 |
排序方式: 共有812条查询结果,搜索用时 15 毫秒
1.
Jeffrey R. Crass MD Ronald L. Genovese VMD James A. Render DVM Errol M. Bellon MD 《Veterinary radiology & ultrasound》1992,33(4):206-216
Ultrasonography and MRI have become valuable tools for imaging of tendon injuries. The current study examines the histopathologic basis for the imaging abnormalities. Five injured equine forelimbs and two normal contralateral limbs were studied with high resolution real time ultrasound and MRI. Histologic sections were made and correlated with the diagnostic images. All lesions were readily seen by both modalities. Lesions characterized by hemorrhage, edma, and cellular infiltration were sonolucent on ultrasound and bright on MRI images. MRI returned to normal as fibrogenesis ensued. Ultrasound images remained abnormal until fibrillar reallgnment occured with completion of the healing process. High resolution real time ultrasound and MRI both accurately reflect the tissue abnormalities in acute tendon injuries. The injured horse does appear to be an effective model for correlative imaging studies of tendon injuries. 相似文献
2.
George J. Brewer MD William Schall DVM Robert Dick BS Vilma Yuzbasiyan-Gurkan PhD Michael Thomas DVM George Padgett DVM 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1992,6(1):41-43
Inherited canine copper toxicosis is a serious problem in Bedlington terriers and West Highland White terriers, and may also be a problem in other less-studied breeds. Affected dogs become ill at midlife with progressive and ultimately fatal liver disease. Treatments for removal of copper and prevention of copper accumulation are available, but are most effective if begun before the dog becomes ill. Until recently diagnosis has not been available until the dog is 1 year of age, and then only by an invasive liver biopsy with determination of liver copper concentration. The authors studied the use of 64copper for early diagnosis of canine copper toxicosis. Two procedures were evaluated. The first involved measuring the concentration of 64copper in blood 24 hours after oral administration of the radioisotope. At this time, 64copper was associated primarily with ceruloplasmin secreted into the blood by the liver. This procedure is useful in the diagnosis of the human counterpart, Wilson's disease. However, the authors found it to be nondiscriminatory between affected and unaffected dogs. In contrast, the second procedure, which involved measuring 64copper excreted in stool during 48 hours after an intravenous dose of radioisotope, yielded results that differentiated most affected and unaffected dogs. 相似文献
3.
Clifford R. Berry DVM Kathy N. Wright DVM Edward B. Breitschwerdt DVM Jerome M. Feldman MD 《Veterinary radiology & ultrasound》1993,34(1):52-55
A 13-year-old neutered female Yorkshire terrier presented with a history of progressive episodic weakness and disorientation of 4 months duration. Physical and neurologic examinations were normal at presentation. Abdominal ultrasound revealed a mass involving the right adrenal gland. Standard planar scintigraphy was performed at 4, 18, and 24 hours after intravenous injection 185 MBq (5mCi) of 123 I-labeled metaiodobenzylguanidine (123 I-MIBG). An area of focal intense uptake was identified in the area of the right adrenal gland. A pheochromocytoma was confirmed histologically after surgical excision. 相似文献
4.
D G Powell 《Veterinary Clinics of North America: Equine Practice》1991,7(1):27-52
The diagnosis of any viral respiratory disease relies on laboratory procedures to isolate the virus and demonstrate a significant rise in serum antibody titers. To isolate viruses from the upper respiratory tract, it is imperative that nasopharyngeal swabs are obtained from animals in the early acute stage of illness, i.e., during the pyrexic phase when the virus is replicating. Nasopharyngeal swabs must be placed in a virus transport medium and forwarded immediately to the laboratory at refrigerated temperature. Equine influenza, rhinopneumonitis, and equine viral arteritis are the three viral infections causing outbreaks of respiratory disease in North America. African horse sickness, although foreign to North America, could be introduced despite stringent horse importation regulations. Specific antiviral therapy is not available to treat viral respiratory disease in the horse. A variety of inactivated and modified live vaccines, however, are available to prevent clinical disease and the spread of infection caused by the common viral respiratory pathogens. A considerable amount of research is underway to enhance the potency and duration of immunity of the present vaccines against influenza and rhinopneumonitis. This research is directed at defining and characterizing the importance of specific glycoprotein antigens on the surface of the virus, which trigger the various host immune responses, and determining whether they are stimulatory or suppressive. 相似文献
5.
Linda J. Barton DVM Jennifer J. Devey DVM Steven Gorski BSt Louis Mainiero MSt Daniel DeBehnke MD FACEP 《Journal of Veterinary Emergency and Critical Care》1996,6(1):21-28
Ten, anesthetized dogs were instrumented with three pulse oximeter probes; two lingual transmittance probes and one rectal reflective probe. Arterial oxygen desaturation was produced by decreasing the inspired oxygen concentration. Hypotension was produced with an infusion of nitroprusside. Simultaneous pulse oximeter readings (SpO2 ) were compared to co-oximeter measured arterial saturation (SaO2 ) collected over a range of SaO2 (50–100%) and mean arterial pressures (40–100mmHg). Each of the monitors and means of evaluating SpO2 studied provided accurate SpO2 measurements over a range of mean arterial pressure from 40–100mmHg. All of the monitors tested tended to overestimate the SaO2 when the arterial saturation was less than 70%. 相似文献
6.
7.
Profile of Steroid Receptors and Increased Aromatase Immunoexpression in Canine Inflammatory Mammary Cancer as a Potential Therapeutic Target
下载免费PDF全文
![点击此处可从《Reproduction in domestic animals》网站下载免费的PDF全文](/ch/ext_images/free.gif)
PJ De Andrés S Cáceres M Clemente MD Pérez‐Alenza JC Illera L Peña 《Reproduction in domestic animals》2016,51(2):269-275
Canine inflammatory mammary cancer (IMC) has been proposed as a model for the study of human inflammatory breast cancer (IBC). The aims of this study were to compare the immunohistochemical expression of aromatase (Arom) and several hormone receptors [estrogen receptor α (ERα), estrogen receptor β (ERβ), progesterone receptor (PR) and androgen receptor (AR)], in 21 IMC cases vs 19 non‐IMC; and to study the possible effect of letrozole on canine IMC and human inflammatory breast cancer (IBC) in vitro using IPC‐366 and SUM‐149 cell lines. Significant elevations of the means of Arom Total Score (TS), ERβ TS and PR TS were found in the IMC group (p = 0.025, p = 0.038 and p = 0.037, respectively). Secondary IMC tumours expressed higher levels of Arom than primary IMC (p = 0.029). Non‐IMC PR‐ tumours contained higher levels of Arom than non‐IMC PR+ tumours (p = 0.007). After the addition of letrozole, the number of IMC and IBC cells dropped drastically. The overexpression of Arom found and the results obtained in vitro further support canine IMC as a model for the study of IBC and future approaches to the treatment of dogs with mammary cancer, and especially IMC, using Arom inhibitors. 相似文献
8.
Low HC Powell CC Veir JK Hawley JR Lappin MR 《American journal of veterinary research》2007,68(6):643-648
OBJECTIVE: To use PCR assays to determine the prevalence of feline herpesvirus 1 (FHV-1), Chlamydophila felis, and Mycoplasma spp DNA in conjunctival cells collected from cats with and without conjunctivitis; to compare results of conventional and real-time fluorogenic PCR assays for amplification of FHV-1 DNA; and to determine whether copy numbers of FHV-1 DNA are correlated with conjunctivitis. ANIMALS: 55 cats with active conjunctivitis, 39 healthy cats that never had conjunctivitis, and 32 cats with a history of conjunctivitis that had been resolved for at least 3 months. PROCEDURES: Samples were obtained by rolling cotton-tipped applicators on the ventral conjunctiva of awake cats treated topically with proparacaine. The DNA was extracted from the swab specimens and assessed in PCR assays to detect DNA of FHV-1 (fluorogenic PCR assay and conventional PCR assay), Mycoplasma spp (conventional PCR assay), and C felis (conventional PCR assay). RESULTS: Overall prevalence rates of FHV-1, C felis, and Mycoplasma spp as assessed by the conventional PCR assays were 6.7%, 3.2%, and 9.6%, respectively. Percentage concordance between conventional PCR and fluorogenic PCR assays for FHV-1 was 92.5%. There were no significant differences among the 3 groups of cats for the mean copy number of FHV-1 divided by the copy number of glyceraldehyde-3-phosphate dehydrogenase. CONCLUSIONS AND CLINICAL RELEVANCE: Mycoplasma spp were the most prevalent organism detected and was associated with conjunctivitis. This study could not confirm that there are increased copy numbers of FHV-1 DNA in cats with conjunctivitis, compared with the copy numbers for cats without conjunctivitis. 相似文献
9.
Assessment of EST- and genomic microsatellite markers for variety discrimination and genetic diversity studies in wheat 总被引:22,自引:0,他引:22
Fiona Leigh Vince Lea John Law Petra Wolters Wayne Powell Paolo Donini 《Euphytica》2003,133(3):359-366
It is likely that in the near future sequence information from sequencing programmes and EST libraries will generate an abundance
of genic microsatellite markers. This study is focused on the assessment of their likely impact and performance vis-à-vis
their genomic counterparts. Microsatellites from two sources were used to assess the genetic diversity in 56 old and new varieties
of bread wheat on the UK Recommended List. A set of 12 microsatellite markers generated from genomic libraries and 20 expressed
sequence tag (EST)-derived microsatellites were used in the study, and the performance of both marker sets assessed. The EST-derived
or genic microsatellites delivered fingerprints of superior quality, amplifying clear products with few stutter bands. Diversity
levels as revealed bygenic microsatellites are similar to the few published results. The PIC values for the genic markers
were generally lower than those calculated for the genomic microsatellites, though advantages of both marker classes for variety
identification applications are discussed.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
10.
Water, Air, &; Soil Pollution - A study was conducted to investigate the heavy metal contamination of soil and vegetation in the vicinity of industries around Dhaka city in Bangladesh.... 相似文献