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1.
Big bluestem (Andropogon gerardii Vitman) and little bluestem [Schizachyrium scoparium (Michaux) Nash.] are native to the North America and are important forage grasses and ornamental grasses. Both grasses are proposed as ideal biomass producers for cellulosic ethanol production. To apply genetic transformation, which is an important tool for incorporating desirable agronomic traits into plants to both species, however requires an efficient and reproducible regeneration protocol. We used mature caryopses from big and little bluestem as explants and tested the effect of various combinations of 2, 4-dichlorophenoxyacetic acid (2, 4-D) (1, 2, 3, 4 or 5 mg l−1) and kinetin (KT) (0, 0.1 or 0.2 mg l−1) on embryogenic callus induction with LS as the basal medium. The highest percentage of embryogenic calli induction occurred on medium containing 2, 4-D alone at 2 mg l−1 for ‘Bison’ and on medium containing 4 mg l−1 2, 4-D alone for ‘Bonilla’ big bluestem. For little bluestem, the highest percentage of embryogenic callus induction occurred on medium containing 3 mg l−1 2, 4-D plus 0.1 mg l−1 kinetin, suggesting that addition of KT is beneficial. Shoot regeneration took place on LS basal medium without any plant growth regulator for both species, although the addition of KT increased both regeneration frequency and the number of shoots produced per callus. Rooting of shoots reaching about 2 cm long occurred readily with or without α-naphthaleneacetic acid (NAA). Rooted plantlets were all successfully established in the soil. 相似文献
2.
本文用约1000块样地资抖,系统地研究了黑龙江省落叶松人工林的地位指数级导向曲线模型。通过林业统计分析软件Statistica6.0对各落叶松人工林数据进行拟合、分析比较,结果为:从拟合精度、曲线走向与散点分布趋势、残差分布等综合考虑,舒马切尔曲线函数比较适合落叶松人工林地位指数级导向曲线模型的拟合。 相似文献
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继续讨论了一类半线性抛物偏微分方程的反问题,在一定条件下,得到了问题的解的存在性,唯一性,并给出了近似解法。 相似文献
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This paper depicted the physiographic landscape features and natural vegetation situation of study area (the eastern Jilin
Province), and expatiates the definition, basic characters and its development of Ecological Land Classification (ELC). Based
on the combination of relief map, satellite photography for study area and vegetation inventory data of 480 sample sites,
a 5-class and a 15-class ecological land type map was concluded according to 4 important factors including slope, aspect,
vegetation and elevation. Ecological Classification System (ECS) is a method to identify, characterize, and map ecosystems.
The Ecological Land Type (ELT) was examined and applied initially in eastern Jilin Province.
Foundation item: This paper was supported by Chinese Academy of Sciences “100 people” project and the Open Research Station of Changbai Mountain
Forest Ecosystem.
Biography: XIAO Bao-ying (1974-), female, postgraduate in Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016,
P. R. China
Responsible editor: Zhu Hong 相似文献
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A new type of versatile vacuum electric furnace for chemical heat treatment is developed, in which the low vacuum structure heating outside, the NH_3 auto-disintegrating instrument, the technology of ultra-red heating, TTL automation and the diverse distr 相似文献
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