An improved ferritin assay for canine sera

An improved serum ferritin assay for canine serum has been developed. It uses two monoclonal antibodies in a sandwich arrangement. Serum ferritin can be determined on undiluted canine sera with this assay. The recovery of ferritin added to canine serum ranged from 98 to 106%, the within-assay coefficient of variability was 3.3 to 4.5%, and the assay-to-assay variability was 9.8 to 10.2%. Serum ferritin from 61 apparently healthy dogs had a geometric mean of 252 ng/ml, with a range of 80 ng/ml to 800 ng/ml.     

Alterations of selected intestinal enzymes in hamsters with hamster enteritis syndrome.

Portions of duodenum, jejunum, and ileum from 21 hamsters clinically affected with enteritis were examined for enzymes. Utilizing a variety of techniques, the activities of alkaline phosphatase, nonspecific esterases, leucine aminopeptidase, beta-glucuronidase, and methyl green pyroninophilia were determined. The activities of enzymes normally associated with tissue proliferation and active protein synthesis were decreased, whereas those enzymes associated by cytolysis were increased.     

探访印度南部有机茶园

最近,我前往印度南部参观了喀拉拉邦蒙纳山附近的Kundaly有机茶园。当我到达KDHP公司总部时,公司常务董事T.V.Alexander和茶叶种植部总经理J.B.Hudson、茶叶生产部总经理Mr.Mohan C.Varghese一起接待了我。通     

The efficacy of a single chain recombinant equine luteinizing hormone (reLH) in mares: induction of ovulation, hormone profiles, and inter-ovulatory intervals

The objectives of this study were to determine the efficacy of recombinant equine luteinizing hormone (reLH) in shortening the time to ovulation in cycling mares and to determine the effects of treatment on endogenous hormones and inter-ovulatory intervals. In study 1, mares of light horse breeds (3–20 years) were treated with either a vehicle, various doses of reLH, or human chorionic gonadotropin (hCG). Cycling mares were examined by palpation and ultrasound per rectum daily or every 12 h from the time of treatment to ovulation. In studies 2 and 3, jugular blood samples were collected daily or every 12 h from the time of treatment to ovulation for analysis of LH, follicle stimulating hormone (FSH), estradiol-17β (E₂), and progesterone (P₄) by radioimmunoassays (RIA). Increasing doses of reLH (0.3, 0.6, 0.75, and 0.9 mg) showed increasing effectiveness at inducing ovulation within 48 h of treatment. Treatments with the 0.75 and 0.9 mg doses of reLH resulted in 90% and 80% ovulation rates, which were similar to hCG treatment (85.7%). Except for the early rise in LH after treatment with 0.5, 0.65, and 1.0 mg of reLH, hormone profiles appeared to be similar between control and treated cycles. Inter-ovulatory intervals were similar between control and treatment cycles. In conclusion, reLH is a reliable and effective ovulatory agent that does not significantly alter endogenous hormone profiles or affect inter-ovulatory intervals.     

Genotypic and environmental variation in crop yield. A method of estimating the interdependence of the components of yield

Summary Because yield components do not behave as independent attributes the conclusions derived from simple yield analyses are of limited value in plant breeding programmes. By calculating a function W of the variances and covariances of the yield components, it is possible to estimate, on a scale from 0 to 1, the degree to which the variations in yield components were independent of one another in any given experiment. By this approach examples of almost complete compensation among the components (W = 0), of mutual independence of the components (W = 0.5), and of additivity of the components (W = 1.0) have been discovered in original data and in the literature. Reasons, and possible applications for these findings are discussed. Investigations into the sampling properties of W are reported in an appendix.