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1.
Sayed Fathi El-Hawari Norihiko Oyama Yukako Koyama Jun Tamura Takaharu Itami Tadashi Sano Kazuto Yamashita 《Journal of veterinary science (Suw?n-si, Korea)》2022,23(4)
BackgroundProblems associated with using inhalational anaesthesia are numerous in veterinary anaesthesia practice. Decreasing the amount of used inhalational anaesthetic agents and minimising of cardiorespiratory disorders are the standard goals of anaesthetists.ObjectiveThis experimental study was carried out to investigate the sparing effect of intravenous tramadol, lidocaine, dexmedetomidine and their combinations on the minimum alveolar concentration (MAC) of sevoflurane in healthy Beagle dogs.MethodsThis study was conducted on six beagle dogs. Sevoflurane MAC was determined by the tail clamp method on five separate occasions. The dogs received no treatment (control; CONT), tramadol (TRM: 1.5 mg kg-1 intravenously followed by 1.3 mg kg-1 h-1), lidocaine (LID: 2 mg kg-1 intravenously followed by 3 mg kg-1 h-1), dexmedetomidine (DEX: 2 μg kg-1 intravenously followed by 2 μg kg-1 h-1), and their combination (COMB), respectively. Cardiorespiratory variables were recorded every five minutes and immediately before the application of a noxious stimulus.ResultsThe COMB treatment had the greatest sevoflurane MAC-sparing effect (67.4 ± 13.9%) compared with the other treatments (5.1 ± 25.3, 12.7 ± 14.3, and 40.3 ± 15.1% for TRM, LID, and DEX treatment, respectively). The cardiopulmonary variables remained within the clinically acceptable range following COMB treatment, although the mean arterial pressure was higher and accompanied by bradycardia.ConclusionsTramadol-lidocaine-dexmedetomidine co-infusion produced a remarkable sevoflurane MAC-sparing effect in clinically healthy beagle dogs and could result in the alleviation of cardiorespiratory depression caused by sevoflurane. Cardiorespiratory variables should be monitored carefully to avoid undesirable side effects induced by dexmedetomidine. 相似文献
2.
Yuichi Takai Tomoya Sano Takeshi Watanabe Ryo Fukuda 《Journal of toxicologic pathology》2013,26(3):319-323
This report describes the morphological and immunohistochemical characteristics of an
adrenocortical carcinoma with distant metastasis in a Sprague-Dawley rat. Macroscopically,
a single large mass was observed in the adrenal gland, and multiple nodules were noted in
the lung, liver and thyroid. Histologically, the adrenal tumor consisted of a solid growth
of eosinophilic round cells with nuclear atypia. Vascular invasion was present, and
multiple metastatic lesions were also observed in the lungs, liver, and mediastinal lymph
nodes. Immunohistochemically, the nuclei of these tumor cells were positive for
Steroidogenic Factor-1 (SF-1). In the thyroid, tumor cells histologically resembling
adrenal cells were immunohistochemically negative for SF-1 but positive for calcitonin;
thus the lesion was diagnosed as thyroid C-cell carcinoma. From these results, the present
case was diagnosed as adrenocortical carcinoma with distant metastases. SF-1 could be a
valuable marker for the differential diagnosis of adrenocortical tumors versus other
endocrine tumors such as C-cell carcinoma. 相似文献
3.
鹌鹑同工酶多型性的研究 总被引:3,自引:0,他引:3
采用水平板淀粉凝胶电泳法 ,对中国目前大规模饲养的 2个蛋用品系鹌鹑的脏器、肌肉中 1 0个酶基因座多型进行检测 ,结果表明 :朝鲜龙城系和德国红鸟系鹌鹑在 Adh、Es-D、Es、Pgi、6-Pgi、Mpi- 6个基因座位均发现多态 ,在Mdh- 、Mdh- 、Mpi- 、α-Gpd 4个基因座位均为单态。引用国外研究结果 ,对国内外 2 1个鹌鹑群体进行聚类分析 ,结果表明 :野生鹌鹑群紧密聚为一类 ,家养鹌鹑群聚为一类 ,商品用鹌鹑和实验用鹌鹑无明显分化 ,而大体重鹌鹑与一般体重鹌鹑分化较大 ,可能是由于进行体重选择的群体偶然携带了不同于原种群的特定基因 相似文献
4.
Yuki Midorikawa Tomohito Shimizu Tetsuya Sanda Katsuyuki Hamasaki Shigeki Dan Mohammad Tamrin Bin Mohamad Lal Goshi Kato Motohiko Sano 《Journal of fish diseases》2020,43(5):541-549
Mass mortality due to necrosis signs occurred in hatchery-reared zoea stage larvae of the mud crab Scylla serrata in Okinawa, Japan, and a causative bacterium was isolated. In this study, we identified and characterized the bacterium by genome analysis, biochemical properties and pathogenicity. The bacterium was a Gram-negative, non-motile, long rod, forming yellow colonies on a marine agar plate. It grew at 20–33°C (not at 37°C) and degraded chitin and gelatin. Phylogenetic analysis of the 16S rRNA gene sequence identified the bacterium as Aquimarina hainanensis. Genome sequence data obtained from Illumina MiSeq generated 29 contigs with 3.56 Mbp in total length and a G + C content of 32.5%. The predicted 16 chitinase genes, as putative virulence factors, had certain homologies with those of genus Aquimarina. Experimental infection with the bacterium conducted on larvae of four crustacean species, brine shrimp Artemia franciscana, freshwater shrimp Caridina multidentata, swimming crab Portunus trituberculatus and mud crab S. serrata, revealed that this bacterium was highly virulent to these species. The present study suggests that the bacterium caused mass mortality in mud crab seed production was A. hainanensis and can be widely pathogenic to crustaceans. 相似文献
5.
Yohei Nakamura Masahiro Horinouchi Mitsuhiko Sano Takuro Shibuno 《Fisheries Science》2009,75(6):1401-1408
Whilst the importance of seagrass beds as nurseries for coral reef fishes has been clearly recognized, the vast majority of
early studies on fish nursery habitats emphasized the close proximity of the latter to coral reefs. To determine the potential
nursery role of isolated seagrass beds, we investigated the degree to which juvenile emperor fishes (Lethrinidae) utilized
seagrass beds in the presence/absence of adjacent coral habitats at Ishigaki Island (southern Japan), such fishes being known
to use seagrass beds as nurseries. Seagrass beds in close proximity to coral habitats (distance between the two habitats of
50–200 m) had greater densities of lethrinid juveniles than those without adjacent coral habitats (2.5–4 km) for 3 different
sites investigated, although a significant difference was obtained only for 1 site. Juveniles of Lethrinus atkinsoni, L. obsoletus, L. harak, and L. nebulosus were observed in seagrass beds with and without adjacent coral habitats, whereas L. ornatus occurred only in the former. Overall, most lethrinid juveniles utilize seagrass beds irrespective of the presence of adjacent
adult coral habitat, suggesting that both types of seagrass beds would contribute to lethrinid adult populations. Therefore,
management efforts for lethrinid populations should be applied not only to contiguous coral-seagrass habitat systems but also
to isolated habitats. 相似文献
6.
Aggregates and gel network structure of globin hydrolysates 总被引:4,自引:0,他引:4
Liu XQ Ohtani T Nakajima M Xu QY Ogawa Y Sano Y 《Journal of agricultural and food chemistry》2001,49(5):2518-2522
A gel with excellent functional properties was prepared successfully using the hydrolysates of globin. In the present study, the structures of intermediate aggregates and gel network were observed directly with an electronic microscope. It was shown clearly that the intermediate aggregates were in a thin rod shape with a length of 130--140 nm, which was in good accordance with the results of the light scattering obtained in a previous study. The diameter of intermediate aggregates was 4--5 nm. Each unit of the intermediate aggregate was composed of beta-chain and peptide beta-1 in a ratio of 1:1. Its molecular weight was 26922 Da, and it had a diameter of 4.1 nm. The thin rod-shaped aggregates were formed with units through the hydrophobic interaction. The length of intermediate aggregate was >30--33 times the diameter. Furthermore, the cross-linked structure formed by peptide alpha-1 and the thin rod-shaped aggregates was also confirmed by the photography of the electronic microscope. These results supported the model proposed in previous papers as proper to depict exactly the formation and structure of the gel network of globin hydrolysates. 相似文献
7.
The effects of dietary crude protein (CP) level and cold exposure on tissue responsiveness and sensitivity to insulin were studied in sheep. Nine rams were assigned to one of three isoenergetic diets which contained 70, 100, and 140% of CP for maintenance. They were exposed from a thermoneutral environment (20 degrees C) to a cold environment (0 degrees C) for 7 days. A hyperinsulinemic euglycemic clamp approach was applied for the determination of tissue responsiveness to insulin (the maximal glucose infusion rate, GIRmax) and tissue sensitivity to insulin (the plasma insulin concentration at half maximal glucose infusion rate, ED50). Dietary CP level influenced digestibilities of dry matter and CP (P=0.002 and P=0.001, respectively), and cold exposure decreased (P=0.01) CP digestibility. The GIRmax and ED50 tended to be influenced (P=0.08) by dietary CP level. The GIRmax was enhanced (P=0.0001) during cold exposure. Significant interactions between diet and environment were found for the GIRmax (P=0.04), but not for ED50 (P=0.07). It is concluded that in sheep dietary CP level can modify insulin action in response to cold exposure. 相似文献
8.
In epidemiological surveys on Chrysanthemum chlorotic mottle viroid (CChMVd) in various chrysanthemums cultivated in Akita Prefecture, Japan, in 2002–2005, approximately 20% of cultivars harbored
CChMVd, including more symptomatic types than nonsymptomatic. Large-flowered cultivars were less frequently infected than
small-flowered and spray types. The number of CChMVd-infected chrysanthemums is increasing, and the disease is found throughout
major chrysanthemum-producing districts. Chrysanthemums infected only with CChMVd, in general, had no noticeable symptoms
of disease. Most of those dually infected with known viruses and/or viroid also had no symptoms characteristic of chlorotic
mottle disease. The lack of noticeable symptoms in major Japanese cultivars may have resulted in the unnoticed spread of the
viroid. 相似文献
9.
Nishifuji K Ueda Y Sano A Kadoya M Kamei K Sekiguchi M Nishimura K Iwasaki T 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2005,52(9):478-480
A 5-year-old male Siberian husky bred outdoor in Tokyo had a swollen paw with interdigital granulomatous lesions in the left hindlimb. The dog had no apparent pulmonary or gastrointestinal involvement. Histopathological analysis of the skin lesions demonstrated yeast-like organisms predominantly within macrophages. Sequence analysis of fungal ribosome RNA gene isolated from a paraffin sample revealed a 100% homology with the teleomorph of Histoplasma capsulatum. The present case may support the concept of primary cutaneous canine histoplasmosis as an endemic phenotype recognized in Japan. 相似文献
10.
Kakizaki T Hamada N Sakashita T Wada S Hara T Funayama T Hohdatsu T Natsuhori M Sano T Kobayashi Y Ito N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(6):605-609
An analysis of ionizing radiation-induced damage in peripheral lymphocytes has been employed to predict the prognosis of radiotherapy in terms of toxicity in normal tissues. Therefore, understanding the sensitivity of lymphocytes to high linear energy transfer (LET)-charged particles would be indispensable for utilizing charged particle therapy in veterinary medicine. However, the availability of such information is very limited. This study aimed to compare the radiosensitivity of feline T lymphocytes to gamma-rays (0.2 keV/microm) and 4 different types of charged particles with LET values ranging from 2.8 to 114 keV/microm. It was observed that the relative biological effectiveness, inactivation cross-section, and isodose-induced apoptosis increased in an LET-dependent manner. On the other hand, no difference in apoptosis frequency was observed in the cells exposed to an isosurvival dose of all the radiation types tested. This is the first study that demonstrates the LET dependence of cell killing and apoptosis induction in feline T lymphocytes. Our results suggest that lymphocytes can be effectively used to predict the prognosis of charged-particle therapy in cat patients. 相似文献