Genetic variations in survival of rohu carp (Labeo rohita, Hamilton) after Aeromonas hydrophila infection in challenge tests

A challenge test against Aeromonas hydrophila was conducted using 2062 rohu carp (Labeo rohita) fingerlings obtained from 52 dams and 87 sires (87 full-sib families) of two year-classes (2003 and 2004). Attempts to establish a cohabitant challenge model were not successful. Therefore, fish were challenged by intraperitoneal injection with A. hydrophila in two replicate tanks per year-class, and dead fish were collected hourly. The mortality reached its peak at 16–22 h after challenge and had almost completely stopped after 58 h. The test was terminated after 382 h at which the average survival was 43.9 and 48.9% in the two 2003 year-class tanks, and 77.1 and 35.8% in the two 2004 year-class tanks. Heritability estimates for survival were obtained from sire and dam threshold models (THR) and sire and dam linear repeatability models (LINR). For both types of models the heritability estimates based on data from single tanks were not consistent. For both year-classes, data from one of the two challenged tanks demonstrated significant additive genetic variation in survival during the A. hydrophila infection, whereas the heritability estimates were not significantly different from zero for the other tank. Further, genetic correlation between survivals in the two replicate tanks in each year-class was not significantly different from zero. The differential results from the replicate tanks demonstrate that additional challenge test experiments are needed before firm conclusions can be drawn about the magnitude of additive genetic variation for survival to aeromonasis in rohu carp. A cohabitant challenge model that allows the testing of important defence mechanisms in the skin and mucous membranes of the fish might have been more appropriate. To establish a valid cohabitant challenge model for rohu carp should be given high priority.     

Classifying tree planters and non planters in a subsistence farming system using a discriminant analytical approach

Since the 1970s tree planting has been promoted to meet the multipurpose needs of subsistence farmers and to arrest deforestation in many developing countries. Financial support and extension systems were developed as tree growing outside the forest became more important. The limited success of the forest extension approaches used in the 1980s has been attributed to the failure to ascertain householders' priorities and attitudes to tree growing. Although the broader theoretical paradigm of factors influencing householders' planting of trees has been discussed household and regional level analyses are rare. This study used discriminant analysis to assess the extent to which social and economic factors affect smallholder farmer tree planting in Orissa, eastern India. The validity of the predictive model to define the different groups was tested by assessing the accuracy of classification. The significance of the factors was probed using the identified variables in the discriminant functions developed for smallholder farmers in the coastal and inland zones of Orissa. The results contradicted some common assumptions that only large landowners with a substantial income are innovators. The likelihood of adopting agroforestry is dependent on the progressive attitude of farmers, membership of village organisations, their wealth status and, more importantly, their perceived risk concerning agricultural production. This approach enables tree planters and non-planters to be characterised and hence give better targetting of planning and social forestry programmes.This revised version was published online in November 2005 with corrections to the Cover Date.     

Bright Spots, Structure, and Magmatism in Southern Tibet from INDEPTH Seismic Reflection Profiling

INDEPTH seismic reflection profiling shows that the decollement beneath which Indian lithosphere underthrusts the Himalaya extends at least 225 kilometers north of the Himalayan deformation front to a depth of approximately 50 kilometers. Prominent reflections appear at depths of 15 to 18 kilometers near where the decollement reflector apparently terminates. These reflections extend north of the Zangbo suture to the Damxung graben of the Tibet Plateau. Some of these reflections have locally anomalous amplitudes (bright spots) and coincident negative polarities implying that they are produced by fluids in the crust. The presence of geothermal activity and high heat flow in the regions of these reflections and the tectonic setting suggest that the bright spots mark granitic magmas derived by partial melting of the tectonically thickened crust.     

Myophosphorylase deficiency (glycogen storage disease Type V) in Charolais cattle

Extract

Myophosphorylase deficiency (glycogen storage disease Type V) has been diagnosed in Charolais calves in New Zealand. It is manifested as exercise intolerance and recumbency, usually at several weeks of age, due to an inability to mobilise glucose, as glucose phosphate, from glycogen. Affected calves may rise and walk after a rest to again become affected on further exercise. There is rhabdomyolysis and after repeated or severe attacks the calf may remain recumbent and need to be euthanised. The mutant phosphorylase gene has been described. We have established a PCR-RFLP test based on a published method, and have confirmed the diagnosis in affected calves and heterozygous status of sires, dams and other related individuals belonging to an extended family. In addition, several other non-related heterozygotes have been identified in that and another herd. There appears to have been importations of the gene from both England and America.     

Myophosphorylase deficiency (glycogen storage disease Type V) in a herd of Charolais cattle in New Zealand: Confirmation by PCR-RFLP testing

AIM: To describe a disease of muscle in Charolais calves and confirm the putative diagnosis of inherited myophosphorylase deficiency.

METHODS: Variously stained paraffin sections of muscle prepared from affected calves were used to describe the lesions. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) test was developed and applied to affected calves, their sires, dams and other individuals.

RESULTS: The lesions were those of rhabdomyolysis of skeletal muscles and sub-sarcolemmal spaces in normal fibres. The PCR-RFLP test confirmed the expected mutation for phosphorylase deficiency of Charolais cattle in two affected calves. In addition, sires, dams and other closely-related individuals of four affected calves tested as heterozygous for the mutation. Other apparently unrelated animals also tested as heterozygous.

CONCLUSIONS: The diagnosis of myophosphorylase deficiency was confirmed. The PCR-RFLP test is suitable for use in controlling this recessively-inherited disorder as it can diagnose heterozygous individuals that are otherwise clinically normal.     

Visible quantum cutting in LiGdF4:Eu3+ through downconversion

For mercury-free fluorescent lamps and plasma display panels, alternative luminescent materials are required for the efficient conversion of vacuum ultraviolet radiation to visible light. Quantum cutting involving the emission of two visible photons for each vacuum ultraviolet photon absorbed is demonstrated in Eu3+-doped LiGdF4 with the concept of downconversion. Upon excitation of Gd3+ with a high-energy photon, two visible photons can be emitted by Eu3+ through an efficient two-step energy transfer from Gd3+ to Eu3+, with a quantum efficiency that approaches 200 percent.     

Impact evaluation of SGSY on socio-economic development of women in aquaculture in Eastern Hills of Orissa

Statistical evaluation of the impact of the SwarnJayanti Gram Swarozgar Yojana (SGSY) on socio-economic development of women in aquaculture in Eastern Hills of Orissa was made. A sample size of 60 women self-help groups (WSHGs) was selected from Koraput and Rayagada districts by multistage stratified random sampling method. The data on socio-economic status of the WSHGs were collected from 2006–2007 to 2008–2009. The members of the WSHGs were interviewed to collect information on their financial contribution and its expenditure pattern. There was a high significant difference between two means in yield (410.9 kg/acre, 306.1 kg/acre), employment generation (387 man-days, 277 man-days), financial contribution by the members to their families (Rs. 3,265/-, Rs. 2,490/-) and expenditure on food (Rs. 953/-, Rs. 538/-) of two groups of WSHGs—the treatment group, i.e., WSHGs under SGSY and the control group. By evaluating the level of development of the WSHGs during 2008–2009, it is revealed that 27.8% of WSHGs under SGSY achieved high level of development in economic sector against 11.9% of WSHGs in the control group. Similarly, in social sector, the percentage of WSHGs in treatment group in the category of high level of development (33%) was higher as compared to the percentage of WSHGs in the control group (11.9%). No significant difference in the development level of empowerment was found between these two groups of WSHGs. The state government should take necessary steps for increasing literacy and technical trainings of the members of the WSHGs in addition to micro-finance for successful implementation of SGSY.