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Yuma SAHASHI Masaaki OSHIMA Junjiro YAMAGISHI Chieko MURAMATSU Kaori SHIMIZU Yasuo INOSHIMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(11):1730
Polymerase chain reaction (PCR) combined with restriction fragment length polymorphism (RFLP) is commonly used for genotyping bovine leukemia virus (BLV) in slaughterhouses. However, unclassified BLV genotypes have been sporadically reported. To assess the current status of BLV genetic characterization in cattle, PCR-RFLP was performed on blood samples of 170 cattle (84 Japanese Black, 60 Japanese Black x Holstein, and 26 Holstein) from 17 farms (5 prefectures) at a slaughterhouse in Aichi Prefecture in 2019. A total of 65 samples (38.2%) were BLV positive, and genotype 1 was the most predominant (56/65 samples), followed by genotypes 3 (6 samples) and 5 (1 sample), and two unclassified samples. No relationship between the genotypes and breeds was observed. Sequence and phylogenetic analyses demonstrated that unclassified BLV genotypes clustered with genotype 1 sequences were, therefore, not new genotypes. 相似文献
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Mikiko FUKUDA Mayuko INOUE Daisuke MURAMATSU Hitoshi MIYACHI Yoichi SHINKAI 《The Journal of reproduction and development》2016,62(1):121-125
Production of knockout mice using targeted embryonic stem cells (ESCs) is a powerful approach for
investigating the function of specific genes in vivo. Although the protocol for gene
targeting via homologous recombination (HR) in ESCs is already well established, the targeting efficiency
varies at different target loci and is sometimes too low. It is known that knockdown of the Bloom syndrome
gene, BLM, enhances HR-mediated gene targeting efficiencies in various cell lines. However,
it has not yet been investigated whether this approach in ESCs is applicable for successful knockout mouse
production. Therefore, we attempted to answer this question. Consistent with previous reports,
Blm knockdown enhanced gene targeting efficiencies for three gene loci that we examined by
2.3–4.1-fold. Furthermore, the targeted ESC clones generated good chimeras and were successful in germline
transmission. These data suggest that Blm knockdown provides a general benefit for efficient
ESC-based and HR-mediated knockout mouse production. 相似文献
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Kanako ISHIHARA Mieko SAITO Natsumi SHIMOKUBO Yasukazu MURAMATSU Shigeki MAETANI Yutaka TAMURA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(12):1627-1629
Veterinary staff carrying
methicillin-resistant Staphylococcus aureus(MRSA) can be a source of MRSA
infection in animals. To identify risk factors of MRSA carriage among veterinary staff,
MRSA carriage and epidemiological information (sex, career, contact with MRSA-identified
animal patients and others) were analyzed from 96 veterinarians and 70 veterinary
technicians working at 71 private veterinary clinics in Japan. Univariate analysis
determined sex (percentage of MRSA carriage, male (29.2%) vs. female (10%);
P=0.002) and career (veterinarians (22.9%) vs. veterinary technicians
(10%); P=0.030) as risk factors. Multivariable analysis revealed that sex
was independently associated with MRSA carriage (adjusted odds ratio, 3.717; 95%
confidence interval, 1.555–8.889; P=0.003). Therefore, male veterinary
staff had a higher risk of MRSA carriage than female staff. 相似文献
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