Production of aromatic amino acids and related compounds from p-hydroxyphenylacetic acid by rumen microorganisms in vitro

Suspensions of mixed rumen bacteria (B), protozoa (P), and mixed rumen microorganisms (BP) prepared from rumen contents of fistulated goats were anaerobically incubated with 1 mM p‐hydroxyphenylacetic acid (HPA) at 39°C for 24 h. Tyrosine (Tyr), phenylalanine (Phe), tryptophan (Trp) and other related compounds in both supernatants and hydrolyzates of microbial cells in all incubations were analyzed by HPLC. Large amounts of Tyr (32.1, 42.7 and 36.1% of disappeared HPA in B, P and BP, respectively) were produced from HPA during a 12 h incubation period. The formation of Tyr in P (178.6 µmol/g MN) was 1.5 and 2 times higher than in B and BP, respectively. Phe (7–11% of the disappeared HPA) and Trp (3–6% of the disappeared HPA) were also synthesized from HPA in B, P, and BP. Phe synthesis in P (46.3 µmol/g MN) was 1.7 times higher than in B but, in contrast, Trp synthesis in B, was 1.6 times higher than in P. The metabolites p‐hydroxyphenylpyruvic acid (in the range of 5–14% of disappeared HPA), phenylacetic acid (1–11%), p‐hydroxybenzoic acid (3–7%) and benzoic acid (1–6%) were produced from HPA in B, P and BP. Phenylpropionic acid (6% of the disappeared HPA) was produced only in B and BP.     

Antimicrobial activity of a bovine myeloid antimicrobial peptide (BMAP‐28) against methicillin‐susceptible and methicillin‐resistant Staphylococcus aureus

A bovine myeloid antimicrobial peptide (BMAP‐28) is a member of the cathelicidin family which is included in the innate immune system of mammals. Recently, there have been many studies about antimicrobial peptides. This study aims to clarify whether BMAP‐28 has bactericidal activity against methicillin‐resistant Staphylococcus aureus (MRSA) and compares its activity against methicillin‐susceptible S. aureus (MSSA) and MRSA. We found that the peptide was effective in killing MRSA (minimal inhibitory concentration (MIC) range; 5–20 µg/mL). It was also revealed that MSSA (MIC range; 1.25–20 µg/mL) had two levels of susceptibility to BMAP‐28. We also examined the effect of BMAP‐28 on bacterial shape to visually show its activity. After exposure to the peptide, both MSSA and MRSA cells showed the morphological changes on their surfaces. Our results indicate that BMAP‐28 is a promising candidate for medicine against drug‐resistant bacteria.     

Effect of stand density and humus removal on regeneration of Pinus densiflora seedlings in a mixed conifer and broad-leaved forest

We studied the impact of stand density and forest floor humus removal on regeneration of Pinus densiflora in a mixed conifer and broad-leaved forest on the Daimonji-Yama Mountain,Kyoto,Japan.Three levels of stand density were set,i.e.,a clear-cut site,a heavily thinned site and a lightly thinned site.In each density treatment,comparisons were carried out between humus removal (A 0 -free)and humus intact(A 0 -intact)subplots.We counted the emergence of sown pine seeds and measured survival and growth of pine seedlings over the next four years.In addition,light conditions and the recovery of understory were monitored.Results show that thinning intensity and humus removal promoted the establishment and growth of seedlings.One exception,however,was that seedling growth was minimal in the heavily thinned A 0 -intact subplots.This was due to a strong response of understory vegetation growth on the treatment combination,which hindered the pine seedling growth.Furthermore,we found that the canopy openness measured at 1.3-m height remained at least 35%for the heavily thinned site in 2008.The results suggest that the removal of the A 0 layer after heavy thinning is the most effective and labor-saving operation for P.densiflora regeneration.     

CHARACTERIZATION OF CANINE FOCAL LIVER LESIONS WITH CONTRAST-ENHANCED ULTRASOUND USING A NOVEL CONTRAST AGENT—SONAZOID

Contrast-enhanced ultrasound using Sonazoid, a novel contrast medium with a liver-specific Kupffer phase, was evaluated in canine focal liver lesions Twenty-five dogs with a liver mass were given intravenous Sonazoid, and the enhancement pattern in the arterial, portal, and parenchymal phase was characterized. An enhancement defect in the lesion in the parenchymal phase was observed in all malignant lesions, whereas only one of nine benign lesions had a filling defect. The diagnostic value of the presence of a filling defect for malignancy was statistically significant (100% sensitivity, 88.9% specificity, 94.1% positive predictive value, 100% negative predictive value), and was equal to that of hypoenhancement in the portal or delayed phase. The defect pattern (clear or irregular defect) was dependent ( P <0.05) on the types of malignancy (i.e., hepatocellular carcinoma and other types of malignancies). In the arterial phase, five of the six hepatocellular carcinomas had hypervascularity, whereas no other lesion was characterized by hypervascularity. In some dogs, additional lesions that could not be observed with conventional B-mode ultrasonography were detected in the parenchymal phase. The enhancement pattern of Sonazoid, especially in the parenchymal phase, has potential as a diagnostic tool for canine focal liver lesions.     

Decadal changes in the diet of northern fur seal (Callorhinus ursinus) migrating off the Pacific coast of northeastern Japan

Decadal changes in northern fur seal (Callorhinus ursinus) diet were examined based on the stomach contents data collected off the Pacific coast of northeastern Japan from January to April, 1953–1988. Seventeen families of fish and seven families of squid were identified from the stomach contents. Dominant prey species in terms of percentage of occurrence and wet weight were Japanese sardine (Sardinops melanostictus), chub mackerel (Scomber japonicus), and myctophid fishes. Demersal fishes, sparkling enope squid (Watasenia scintillans), and oceanic squids were also preyed on at low incidences. Decadal‐scale diet composition of northern fur seals revealed shifts in the significance of Japanese sardine and chub mackerel in parallel with the decadal alternation in the dominance of these species within the pelagic fish community off the Pacific coast of northeastern Japan. These results suggest that northern fur seals can use a variety of prey resources in this wintering area by switching the diet according to the distribution and abundance of prey species.     

Total activity of transglutaminase at various temperatures in several fish meats

ABSTRACT: Transglutaminase seems to be related to the setting phenomenon of fish meat paste that occurs at temperatures below 40°C. In many reports on the relationship between transglutaminase and setting phenomenon, the enzyme activity has been measured at 25°C. However, it is known that the setting phenomenon is complicated and the effect of calcium and the 3-D structure of myofibrils, which are sensitive to temperature, play important roles in the reaction. In the present study, total activities of transglutaminase of threadfin bream, white croaker, red sea bream and carp meats were measured at various temperatures. Total transglutaminase activities at 25°C of tested fish meats are arranged in order as follows; white croaker, red sea bream, carp, threadfin bream. In contrast, optimal temperature of carp meat is 30°C, red sea bream 40°C, threadfin bream 50°C and white croaker 50–55°C. In carp meat, the enzyme activity at optimal temperature (30°C) became 8.5-fold higher than at 25°C. The data of the total activity at various temperatures are useful in order to comprehend the details of the reaction.     

利用柽柳改良盐碱地土壤的机制与措施初报

柽柳通过根系从土壤中吸收盐分，通过体内运输，将盐分主要蓄积在叶和细枝当中并排出体外。因此，通过各种生物学、生态学和栽培学措施促进柽柳繁茂地生长，以获得大量的细枝和叶的生物量，可以有效地降低土壤中的盐分含量，达到改良和利用盐碱地的目的。     

Contraceptive effect of a gonadotropin-releasing hormone vaccine on a captive female African Lion (Panthera leo): a case study

Lions (Panthera leo) breed well under captivity, so contraception has been commonly conducted for population management, leading to a demand for a less invasive and reversible contraceptive approach in lions. In this study, we examined the efficacy of a commercial gonadotropin-releasing hormone vaccine as a method of suppressing reproductive activity in a sexually matured female lion. Under behavioral restraint, the vaccine was injected twice (days 0 and 109). After the initial vaccination, ovarian activity is still observed. After the second vaccination, contraceptive effect was confirmed for 246 days until restart of estrous cycles. We confirmed only a slight swelling around the injection site after the second vaccination. This study may suggest an alternative option for a contraceptive method in lions.