全文获取类型
收费全文 | 433篇 |
免费 | 27篇 |
专业分类
林业 | 16篇 |
农学 | 2篇 |
基础科学 | 1篇 |
95篇 | |
综合类 | 50篇 |
农作物 | 69篇 |
水产渔业 | 43篇 |
畜牧兽医 | 168篇 |
园艺 | 4篇 |
植物保护 | 12篇 |
出版年
2022年 | 3篇 |
2021年 | 3篇 |
2020年 | 3篇 |
2019年 | 5篇 |
2018年 | 4篇 |
2017年 | 7篇 |
2016年 | 7篇 |
2015年 | 6篇 |
2014年 | 9篇 |
2013年 | 14篇 |
2012年 | 10篇 |
2011年 | 30篇 |
2010年 | 11篇 |
2009年 | 11篇 |
2008年 | 13篇 |
2007年 | 17篇 |
2006年 | 20篇 |
2005年 | 23篇 |
2004年 | 20篇 |
2003年 | 22篇 |
2002年 | 42篇 |
2001年 | 12篇 |
2000年 | 8篇 |
1999年 | 11篇 |
1998年 | 3篇 |
1997年 | 8篇 |
1996年 | 9篇 |
1995年 | 4篇 |
1994年 | 3篇 |
1993年 | 7篇 |
1992年 | 4篇 |
1991年 | 3篇 |
1990年 | 3篇 |
1989年 | 6篇 |
1988年 | 5篇 |
1985年 | 4篇 |
1981年 | 4篇 |
1975年 | 3篇 |
1948年 | 4篇 |
1947年 | 8篇 |
1946年 | 4篇 |
1945年 | 3篇 |
1943年 | 2篇 |
1937年 | 5篇 |
1935年 | 3篇 |
1934年 | 3篇 |
1930年 | 4篇 |
1926年 | 2篇 |
1925年 | 2篇 |
1924年 | 2篇 |
排序方式: 共有460条查询结果,搜索用时 15 毫秒
1.
2.
Daniel J. Burba DVM Michael A. Collier DVM Lawrence E. Default PhD Olivia Hanson-Painton PhD Harold C. Thompson Jr. Claude L. Holder 《Journal of Equine Veterinary Science》1993,13(12):696-703
The uptake and distribution of intramuscularly (IM) administered tritium-labeled polysulfated glycosaminoglycan (3H-PSGAG) in serum, synovial fluid, and articular cartilage of eight horses was quantitated, and hyaluronic acid (HA) concentration of the middle carpal joint was evaluated in a pharmacokinetic study. A full-thickness articular cartilage defect, created on the distal articular surface of the left radial carpal bone of each horse served as an osteochondral defect model. 3H-PSGAG (500 mg) was injected IM, between 14 and 35 days after creation of the defects. Scintillation analysis of serum and synovial fluid, collected from both middle carpal joints at specific predetermined times up to 96 hours post-injection, revealed mean 3H-PSGAG concentrations peaked at 2 hours post-injection. 3H-PSGAG was detected in cartilage and subchondral bone 96 hours post-injection in samples from all eight horses. There were no statistically significant differences in 3H-PSGAG concentration of synovial fluid or cartilage between cartilage defect and control (right middle carpal) joints.
HA assay of synovial fluid revealed concentrations significantly increased at 24, 48, and 96 hours post-injection in both joints. The concentration nearly doubled 48 hours post-injection. However, no statistically significant differences were found between synovial concentrations of HA in cartilage defect and control joints.
3H-PSGAG administered IM to horses, was distributed in the blood, synovial fluid, and articular cartilage. HA concentrations in synovial fluid increased after IM administration of polysulfated glycosaminoglycan. 相似文献
3.
Walter GL McGraw P Tvedten HW 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1992,21(1):23-27
An enzymatic, kinetic method for determining serum lipase activity was evaluated and compared to a standard manual method for use in dogs. The kinetic method was a commercial kit adapted for use on a tandem access clinical chemistry analyzer and utilized a series of coupled enzymatic reactions based on the hydrolysis of 1,2-diglyceride by lipase. The manual method was the Cherry-Crandall technique based on the titration of base against the acid formed by hydrolysis of an olive oil substrate by lipase. The correlation between the two methods was very good (r = 0.94). The reference range for 56 clinically healthy dogs assayed by the kinetic method was 90 to 527 U/L. Diseases associated with a greater than twofold elevation in serum lipase activity as determined by the kinetic method included pancreatitis, gastritis with liver disease, and oliguric renal failure with metabolic acidosis. In some cases, pancreatitis was seen with other clinical problems, such as gastroenteritis, diabetic ketoacidosis, duodenal mass, disseminated intravascular coagulation, and septic peritonitis. Diseases associated with serum lipase activity within the reference range or elevated less than twofold included gastritis, gastric ulcer, cholestasis, phenobarbital-induced hepatopathy, colitis, copper hepatopathy, abdominal hematoma, apocrine gland adenocarcinoma, and thrombocytopenia with pneumonia. 相似文献
4.
5.
6.
Krishnan HB Jang S Kim WS Kerley MS Oliver MJ Trick HN 《Journal of agricultural and food chemistry》2011,59(4):1223-1228
Legumes, including soybeans ( Glycine max ), are deficient in sulfur-containing amino acids, which are required for the optimal growth of monogastric animals. This deficiency can be overcome by expressing heterologous proteins rich in sulfur-containing amino acids in soybean seeds. A maize 27 kDa γ-zein, a cysteine-rich protein, has been successfully expressed in several crops including soybean, barley, and alfalfa with the intent to biofortify these crops for animal feed. Previous work has shown that the maize 27 kDa zein can withstand digestion by pepsin and elicit an immunogenic response in young pigs. By use of sera from patients who tested positive by ImmunoCAP assay for elevated IgE to maize proteins, specific IgE binding to the 27 kDa γ-zein is demonstrated. Bioinformatic analysis using the full-length and 80 amino acid sliding window FASTA searches identified significant sequence homology of the 27 kDa γ-zein with several known allergens. Immunoblot analysis using human serum that cross-reacts with maize seed proteins also revealed specific IgE-binding to the 27 kDa γ-zein in soybean seed protein extracts containing the 27 kDa zein. This study demonstrates for the first time the allergenicity potential of the 27 kDa γ-zein and the potential that this protein has to limit livestock performance when used in soybeans that serve as a biofortified feed supplement. 相似文献
7.
8.
9.
10.