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Beatriz Wiebke-Strohm Annette Droste Giancarlo Pasquali Marina Borges Osorio Lauro Bücker-Neto Luciane Maria Pereira Passaglia Marta Bencke Milena Schenkel Homrich Márcia Margis-Pinheiro Maria Helena Bodanese-Zanettini 《Euphytica》2011,177(3):343-354
An Agrobacterium-mediated transformation procedure for soybean [Glycine max L. Merrill] proliferating somatic embryos is here described. The Agrobacterium tumefaciens LBA4404 strain harboring pTOK233, pCAMBIA1390-olp or pH7WG2Dwrky plasmids was used to mediate gene transfer into the plant genome. Prior to Agrobacterium inoculation, proliferative soybean embryogenic clusters were microwounded by DNA-free tungsten particle bombardment. Three
independent transformation experiments were performed. In Experiment I, 26 transgenic plants were obtained from a unique clone
of cv Bragg, while 580 plants were recovered from 105 clones of cv IAS5. In Experiment II, a single hygromycin-resistant clone
of cv BRSMG68 Vencedora was recovered and gave rise to five plants. In Experiment III, 19 plants of cv Bragg and 48 plants
of IAS5 were recovered, representing five and 14 independent transformation events, respectively. PCR and Southern analyses
confirmed the transgenes’ integration into plant genomes. Transgenic plants were fertile. They flowered, set pods and seeds.
Transgene segregation in two T1 progenies fits the Mendelian pattern (3:1 transgenic:non-transgenic plants). This is the first report of transgenic fertile
soybean plants obtained from somatic embryogenic tissues transformed by the system that combines DNA-free particle bombardment
and Agrobacterium. 相似文献
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Picerno P Mencherini T Lauro MR Barbato F Aquino R 《Journal of agricultural and food chemistry》2003,51(22):6423-6428
An extract of Xanthosoma violaceum leaves was subjected to a polyphenol profile determination, including total polyphenols, and antioxidant activity evaluation. Analysis of the extract resulted in the isolation of a new flavone C-glycoside, apigenin 6-C-beta-D-glucopyranosyl-8-C-beta-D-apiofuranoside (1), as well as known flavone C-glycosides, including vitexin (2), isovitexin (3), isovitexin 4'-O-rhamnopyranoside (4), apigenin 6-C-[beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranoside] (5), and apigenin 6,8-diC-beta-D-glucopyranoside (6). The antioxidant activity of the extract was assessed by means of two different in vitro tests: bleaching of the stable 1,1-diphenyl-2-picrylhydrazyl radical (DPPH test) and peroxidation induced by the water-soluble radical initiator 2,2'-azobis(2-amidinopropane) hydrochloride, on mixed dipalmitoylphosphatidylcholine/linoleic acid unilamellar vesicles (LP-LUV test). In both tests used, the extract and a fraction II showed a significant antioxidant/free-radical scavenging effect (fraction II, EC(50) = 11.6 microg/mL) in comparison to alpha-tocopherol (EC(50) = 10.1 microg/mL). 相似文献
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Guilherme Pugliesi Danilo Zago Bisinotto Barbara Piffero Mello Fbio C. Lahr Catia A. Ferreira Gabriela Dalmaso Melo Michele Ricieri Bastos Ed Hoffmann Madureira 《Reproduction in domestic animals》2019,54(8):1149-1154
We aimed to evaluate the reproductive performance of Nelore lactating cows submitted to a resynchronization 12 days after timed artificial insemination (TAI) with or without a long‐acting progesterone (P4‐LA) treatment. Nelore cows were submitted to a P4/oestradiol‐based TAI protocol (D0 = insemination). On D12, cows in the control group (n = 184) received a new P4 intravaginal device (0.96 g), whereas cows in the P4‐LA group (n = 192) received the P4 device and 75 mg P4‐LA. Cows identified as non‐pregnant (n = 120) by regression of corpus luteum using colour Doppler ultrasonography on D20 had the P4 device removed and received 500ug of sodium cloprostenol, 1 mg of oestradiol cypionate and 300 IU of eCG and were re‐inseminated on D22. There was no difference (p > 0.10) in the pregnancy rate at D20, D30 and D60 after first TAI between the control (69%, 59.7% and 57%, respectively) and P4‐LA (67%, 55.7%, and 55.2%, respectively) groups. Pregnancy losses were similar between both groups (p > 0.1). For cows submitted to the second TAI, the pre‐ovulatory follicle size did not differ (p > 0.1), but the oestrous detection and pregnancy rates were greater (p < 0.05) in the P4‐LA group (92.2% [59/64] and 60.9% [39/64], respectively) than in controls (75% [42/56] and 44.6% [25/56]). The cumulative pregnancy rate after two TAIs did not differ (p > 0.1) between control (73.3% [135/184]) and P4‐LA (76% [146/192]) groups. The use of P4‐LA at 12 days after TAI potentially increases the pregnancy rates for a new early resynchronization strategy associated with the Doppler imaging for pregnancy diagnosis and results in an alternative to perform two TAIs in 22 days in beef cows. 相似文献
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José Maria Villela Pádua Kaio Olímpio Das Graças Dias Maria Marta Pastina João Cândido de Souza Valéria Aparecida Vieira Queiroz Rodrigo Veras da Costa Maria Beatriz Pereira da Silva Carlos Alexandre Gomes Ribeiro Claudia Teixeira Guimarães Salvador Alejandro Gezan Lauro José Moreira Guimarães 《Euphytica》2016,211(3):277-285
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Glauco Vieira Miranda Leandro Vagno de Souza João Carlos Cardoso Galvão Lauro José Moreira Guimarães Aurélio Vaz de Melo Izabel Cristina dos Santos 《Euphytica》2008,162(3):431-440
The development of successful advanced lines and cultivars in maize is dependent on parental selection and assignment to defined
heterotic groups. So, the objectives of this study were to evaluate genetic variability and identify heterotic groups among
Brazilian popcorn varieties. Thus, diallel crosses of advanced generations of the popcorn hybrids, IAC 112 and Zélia, and
of three open-pollinated popcorn varieties, RS 20, Branco, and SAM were performed. Ten hybrid combinations, the five parents,
and five check treatments were arranged in a block design with four replicates in two tropical-zone locations (CWb climate).
Both additive and non-additive effects were important for grain yield, plant height, ear height, and husk cover. For popping
expansion, only the additive effects were important. Hybrid combinations between the local variety (Branco) and F2 populations (IAC 112 and Zelia) resulted in the third and fourth highest values for popping expansion. The best grain yields
were obtained with hybrid combinations involving SAM. Cultivars Zélia, IAC 112, and RS 20 increased popping expansion, whereas
cultivar Branco increased grain yield of hybrid combinations. The following conclusions may be drawn: Brazilian popcorn populations
have reduced heterosis and genetic variability to popping expansion in relation commercial cultivars; there is genetic variability
among Brazilian popcorn populations that allows the exploitation of additive and non-additive effects for grain yield; it
is possible to increase grain yield by using local varieties; but it would be difficult to obtain commercial hybrids from
local varieties because they have poor performance for popping expansion. 相似文献
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C. Montesissa G. Pompa F. M. di Lauro L. Fadini 《Veterinary research communications》1987,11(1):57-64
Fenclor 64 (a PCB's commercial mixture) was administered twice i. p. to virgin and pregnant New Zealand rabbits at the dose of 100 mg/Kg to evaluate its inducing properties on the following hepatic microsomal MFO activities: p-nitro-anisole-O-demethylase, amino-pyrine-N-demethylase, acetanilide-hydroxylase and arylhydrocarbons-hydroxylase.The results indicate that Fenclor 64 evoked a mixed type induction in virgin as well as in pregnant rabbits since it increased liver weight, cytochrome P-450 levels, as well as p-nitro-anisole-O-demethylase and acetanilide-hydroxylase. Pregnancy by itself affects only amino-pyrine-N-demethylase levels.Work supported by a grant from M. P. I. 40% (1982) 相似文献
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Laura?Yá?ez-Espinosa Teresa?TerrazasEmail author Lauro?López-Mata Juan?I.?Valdez-Hernández 《Wood Science and Technology》2004,38(3):217-226
Laguncularia racemosa wood is commonly used in north-western Mexico and harvested without the knowledge of whether environmental conditions affect wood quality.
Laguncularia racemosa anatomy variation was evaluated to identify which cell features, mainly fibre features, are related to an environmental gradient and how this variation affects wood quality. Trees were sampled in six sites along the river where different flooding periods and levels occur. Analyses revealed that in sites with high salinity and flooding levels, there are more abundant vessels and axial parenchyma although the fibres and vessel elements are shorter, suggesting a water stress effect. Correlation analysis confirmed that the higher the percentage of gelatinous fibres, the longer the fibres. This occurred in L. racemosa trees growing in those sites with a high sand particle percentage and a lower flooding level. Anova (analysis of variance) revealed non-significant differences among sites for flexibility, rigidity and Peteri coefficients as well as for Runkel ratio, however the rigidity coefficient is affected by occurrence of gelatinous fibres. L. racemosa wood harvesting should avoid those sites with high flooding level, high sand particle percentage and high salinity that may modify wood quality. 相似文献
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das Araújo Francisca Chagas Bezerra Moura Elisa Ferreira de Rodrigues Simone Miranda de Carvalho Kenny Bonfim Arruda Tavares Eraldo José Madureira Moura Mônika Fecury da Silva Gilvan Ferreira 《Genetic Resources and Crop Evolution》2021,68(5):1809-1824
Genetic Resources and Crop Evolution - The biofortification of cassava (Manihot esculenta Crantz) roots is one of the actual goals of genetic breeding, with the development of roots richer in... 相似文献
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