Post-translational modifications like ubiquitination determine the fate of many cellular proteins. There are evidences about the role of these ubiquitinated proteins in the antiviral immune response. E3 ubiquitin ligases like RBR (RING between RING) proteins play a crucial role in the regulation of immune signalling. In the present study, two RBR genes (RNF114 and RNF144A) from Asian seabass (Lates calcarifer) were cloned and characterized. Phylogenetic analysis reveals that these genes were closely clustered with their counterparts from teleost origin. Structurally they feature multiple domains with RING1 and RING2 domains and an IBR (in between RING) domain. The challenge studies with poly(I:C) and RGNNV virus gave evidence that both Lates RNF114 and RNF144A genes play a crucial role in antiviral immune response. Our studies suggest that RBR is an important gene in innate immune response and shed light on antiviral immune research in Lates calcarifer and its aquaculture.
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