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排序方式: 共有387条查询结果,搜索用时 15 毫秒
1.
To develop an appropriate cryopreservation protocol for spermatophores of black tiger shrimp, Penaeus monodon, three cryoprotectants (dimethyl sulphoxide (DMSO), methanol (MeOH) and ethylene glycol (EG)) at two concentrations (5% and 10%) were examined. Artificial implantation of spermatophores was also carried out to assess the fertilizing ability of fresh and post‐thaw spermatophores. Spermatophores were collected during consecutive regenerations (15‐day intervals) and assessed for qualitative and quantitative changes and also for fertilizing ability by implantation. The mean fertilization rate for artificial insemination using post‐thaw spermatophore was 79.9±3.7%, lower than the fertilization rates observed for artificial implantation using fresh spermatophore and natural mating. Mean hatch rates for fresh spermatophore, frozen‐thawed spermatophore and natural mating were 88.8±0.6%, 87.8±0.4% and 88.3±0.5%, respectively; and there was no difference among the three groups. The mean fertilization rate of spermatophores collected during the first stripping was higher (90.6±0.6) than during the second stripping (85.7±2.6), but the mean hatch rate was not different between the two strippings. The highest mean sperm viability (79.7±0.4%) was obtained from DMSO (5%), with no survival observed in the 10% MeOH treatment. Spermatophore weight, total sperm count and percentage of abnormal sperm were not different between spermatophores collected at the first and second stripping. This is the first study to report high fertilization and hatch rates from cryopreserved spermatophore using artificial implantation of spermatophore before spawning. 相似文献
2.
Meghnath Pokharel Anuj Chiluwal Michael Stamm Doohong Min Davina Rhodes S.V. Krishna Jagadish 《Journal of Agronomy and Crop Science》2020,206(5):579-596
Winter canola (Brassica napus L.) is highly sensitive to increasing temperatures during the reproductive and pod-filling stages. Although the impact of high day-time temperature stress on yield and quality has been documented in canola, similar information under high night-time temperature (HNT) stress is not available. Using six hybrids and four open-pollinated cultivars, we observed a marked shift in peak flowering towards earlier, cooler hours of the morning under HNT. Averaged across two independent experiments, the photochemical efficiency of photosystem II was significantly decreased (3%), with a significant increase in thylakoid membrane damage (13%) in the leaves of susceptible cultivars under HNT stress. Similarly, the susceptible cultivars also recorded significant reduction in biomass (34%), pod number (22%), pod weight (37%) and total seed weight (40%) per plant while the same set of agronomic traits were not affected among the tolerant cultivars. Quantitative impact of heat stress was confirmed with increased sensitivity to HNT exposure from gametogenesis until maturity resulting in a significantly higher yield loss compared to stress exposure from post-flowering till maturity. HNT significantly decreased oil concentration, but increased protein concentration and saturated fatty acid levels in seeds of the susceptible cultivars. However, HNT had no impact on the unsaturated fatty acids in both hybrids and the open-pollinated cultivars. Breeding targets based on fatty acid composition for enhancing canola seed quality may not be easily amenable due to the inconsistency documented with the compositional changes under heat stress. In summary, our findings conclude that canola hybrids are better suited to regions experiencing heat stress, compared to open-pollinated cultivars, indicating the possibility of a complete shift to hybrid canola cultivation under predicted hotter climates in the future. 相似文献
3.
Thakur Shikha Verma Subhash Chander Sharma Prem Lal Chandel Rajeshwar Singh Sharma Priyanka Sharma Shubham Sharma Prajjwal 《Phytoparasitica》2023,51(2):189-198
Phytoparasitica - Diaeretiella rapae (McIntosh) (Hymenoptera: Braconidae) is one of the most promising, effective and a common endoparasitoid of different aphid species including cabbage aphid,... 相似文献
4.
Mazumder S Morvan C Thakur S Ray B 《Journal of agricultural and food chemistry》2004,52(11):3556-3562
Pectic polysaccharides were obtained from chalkumra (Benincasa hispida) fruit by sequential extraction with ammonium oxalate (fraction BOX), dilute acid (fraction BHCl), and cold dilute alkali (fraction BOH). The highest yield of polysaccharides was obtained with oxalate and HCl. BOX was enriched in partly methyl-esterified galacturonic acid, whereas BHCl and BOH contained mostly galactose. All of the extracts showed similar elution patterns in size exclusion chromatography although the intrinsic viscosities (eta) were different (132 +/- 6, 100 +/- 5, and 285 + 10 mL/g for BOX, BHCl, and BOH, respectively). From fractionation by anion exchange chromatography, homogalacturonan (as seen from sugar analysis and Fourier transform infrared spectrum) accounted for more than half of BOX and 11% of BHCl. Methylation analyses and hydrolysis of BHCl with endo-beta-(1-->4)-d-galactanase showed the presence of beta-(1-->4)-d-galactan. The neutral galactan represented more than 76% of BHCl and approximately 40% of BOH. The other polysaccharides were complex galactans in BOH and an acidic arabinan (<1%) in BOX and BHCl. 相似文献
5.
Yadav Sudhir Mondal Manoranjan K. Shew Aaron Jagadish S. V. Krishna Khan Zahirul Haque Sutradhar Asish Bhandari Humnath Humphreys Elizabeth Bhattacharya Jayanta Parvin Rokhsana Rahman Mahabubur Chandna Parvesh 《Paddy and Water Environment》2020,18(2):331-343
Paddy and Water Environment - Most of the lands of the southern coastal zone of Bangladesh are protected from tidal flooding and storm surges by embankments constructed during the 1960s and 1970s,... 相似文献
6.
Kwasi Bannor Richard Amarnath Krishna Kumar Gupta Oppong-Kyeremeh Helena Abawiera Wongnaa Camillus 《水稻科学》2020,27(1):56-66
The factors affecting the adoption of modern varieties(MVs) of rice and impact on poverty in Odisha, India were discussed. A total of 363 households from Cuttack and Sambalpur districts of Odisha via multistage sampling technique participated in the survey. The Cragg's Double hurdle model was used to model the determinants of adoption and intensity of adoption of MVs of rice, and the propensity score matching was used to analyze the impact of adoption on poverty. The results showed that age, education, risk aversion, land size, yield, perception of MVs as high yielding, resistant to diseases and availability of MVs positively influenced the decision to adopt. However, variables such as household size, experience of a farmer, off-farm job participation, amount of credit received, cost of seeds, insecticides and fertilizers negatively influenced the adoption of MVs. Intensity of adoption of MVs was negatively influenced by experience of a farmer, cost of fertilizer and marketability of MVs, and positively affected by household size, risk aversion, land size, cost of insecticides, perception of MVs as high yielding and availability of MV seeds. Poverty incidence, gap and severity were high among non-adopters to adopters of MVs. After matching adopters and non-adopters of MV groups using four different algorithms of nearest neighbour matching, stratification matching, radius matching and kernel matching, the impact of MV adoption resulted in higher per capita monthly household expenditure by about US$ 52.82 to US$ 63.17. 相似文献
7.
Emilie Laurin Krishna Thakur Peter G. Mohr Paul Hick Mark St. J. Crane Ian A. Gardner Nicholas J. G. Moody Axel Colling Ingo Ernst 《Journal of fish diseases》2019,42(11):1471-1491
Samples from multiple animals may be pooled and tested to reduce costs of surveillance for infectious agents in aquatic animal populations. The primary advantage of pooling is increased population‐level coverage when prevalence is low (<10%) and the number of tests is fixed, because of increased likelihood of including target analyte from at least one infected animal in a tested pool. Important questions and a priori design considerations need to be addressed. Unfortunately, pooling recommendations in disease‐specific chapters of the 2018 OIE Aquatic Manual are incomplete and, except for amphibian chytrid fungus, are not supported by peer‐reviewed research. A systematic review identified only 12 peer‐reviewed aquatic diagnostic accuracy and surveillance studies using pooled samples. No clear patterns for pooling methods and characteristics were evident across reviewed studies, although most authors agreed there is a negative effect on detection. Therefore, our purpose was to review pooling procedures used in published aquatic infectious disease research, present evidence‐based guidelines, and provide simulated data examples for white spot syndrome virus in shrimp. A decision tree of pooling guidelines was developed for use by peer‐reviewed journals and research institutions for the design, statistical analysis and reporting of comparative accuracy studies of individual and pooled tests for surveillance purposes. 相似文献
8.
Sabyasachi Banerjee Seema Singh Shailesh Pandey Maneesh S. Bhandari Amit Pandey Krishna Giri 《Forest Pathology》2020,50(2)
Wilt disease caused by Fusarium solani is a serious constraint to Dalbergia sissoo (shisham) plantations in northern India. In this study, the antagonistic potential of 40 bacterial isolates recovered from rhizophere soil of healthy shisham trees, and a well‐characterized Trichoderma species (Trichoderma virens) were tested for their possibility as biocontrol agents for F. solani. Two promising isolates (S1 and S15) were identified which inhibited pathogen growth, caused chitin degradation, produced siderophores and solubilized phosphate in vitro. Isolate S15 scored highest for hydrogen cyanide (HCN) production while isolate S1 was a non‐HCN producer. These two isolates were identified as Serratia marcescens (S1) and Pseudomonas azotoformans (S15) following sequence analysis of 16S rDNA. In dual culture assays, T. virens caused 80% inhibition of mycelial growth of the test fungus. The three selected antagonists when tested in planta in the glasshouse completely suppressed production of wilt symptoms on 12‐month‐old shisham plants. Further work is needed to ascertain the potential of these isolates to be used as biocontrol agents to manage shisham wilt under field conditions. 相似文献
9.
A. K. Thakur S. Rath S. Roychowdhury N. Uphoff 《Journal of Agronomy and Crop Science》2010,196(2):146-159
The System of Rice Intensification (SRI) reportedly enhances the yields of rice (Oryza sativa L.) through synergy among several agronomic management practices. This study was conducted to investigate the effects on rice plant characteristics and yield by comparing the plants grown with different methods of cultivation – SRI vs. recommended management practices (RMP) focusing on the impact of different plant spacings. Performance of individual hills was significantly improved with wider spacing compared with closer‐spaced hills in terms of root growth and xylem exudation rates, leaf number and leaf sizes, canopy angle, tiller and panicle number, panicle length and grain number per panicle, grain filling and 1000‐grain weight and straw weight, irrespective of whether SRI or RMP was employed. Both sets of practices gave their highest grain yield with the spacing of 20 × 20 cm; however, SRI yielded 40 % more than the recommended practice. At this spacing, canopies also had the highest leaf area index (LAI) and light interception during flowering stage. The lowest yield was recorded at 30 × 30 cm spacing under both the practices, as a result of less plant population (11 m?2), despite improved hill performance. During the ripening stage, hills with wider spacing had larger root dry weight, produced greater xylem exudates, and transported these towards shoot at faster rates. These features contributed to the maintenance of higher chlorophyll levels, enhanced fluorescence and photosynthesis rates of leaves and supported more favourable yield attributes and grain yield in individual hills than in closely‐spaced plants. Moreover, these parameters further improved in SRI, apart from the enhanced percentage of effective tillers and showed substantial and positive impacts on grain yield (17 %) compared with recommended practice. In conclusion, wide spacing beyond optimum plant density, however, does not give higher grain yield on an area basis and for achieving this, a combination of improved hills with optimum plant population must be worked out for SRI. 相似文献
10.
Bordoloi GN Kumari B Guha A Thakur D Bordoloi M Roy MK Bora TC 《Pest management science》2002,58(3):297-302
Screening for newer bioactive compounds from microbial metabolites resulted in the isolation of a novel antibiotic from the culture filtrate, Streptomyces sp 201, of a soil. The bioactive compound, with antifungal and antibacterial activity, was identified as 2-methylheptyl isonicotinate. The antifungal activity of live culture, culture broth and the isolated bioactive compound showed marked inhibition against dominant soil-borne phytopathogens such as Fusarium oxysporum Schlect, F moniliforme Sheldon, F semitectum Berkeley & Ravenel, F solani (Martius) Sacc and Rhizoctonia solani Kuehn. The compound had no effect on seed germination and seedling development as displayed by root and stem growth of the test plant species. In pot experiments with seedlings of cruciferous plants such as Raphanus sativus L (radish), Brassica campestris L (yellow mustard), Brassica oleracea var botrytis L (cauliflower), the antibiotic compound showed promising protective activity of 92% when seeds of the test plants were treated at a dose of 50 micrograms ml-1 prior to sowing. Seed treatment with a spore suspension (3 x 10(8) spores ml-1) of the Streptomyces sp 201 displayed protective activity in the range of 56-60%. Seeds coated with 2.5% methyl cellulose-amended spores of the antagonist showed protective activity in the range of 64-72%. Further, seed treatment with the culture filtrate of the antagonist also showed promising protective activity in the range of 64-84%. 相似文献