排序方式: 共有2条查询结果,搜索用时 0 毫秒
1
1.
Miaomiao Xin Jan Sterba Anna Shaliutina-Kolesova Borys Dzyuba Jaroslava Lieskovska Serhii Boryshpolets Mohammad Abdul Momin Siddique Vitaliy Kholodnyy Ievgen Lebeda Otomar Linhart 《Fish physiology and biochemistry》2018,44(6):1527-1533
The loss of sperm quality in sterlet (Acipenser ruthenus) due to freeze-thaw process in cryopreservation was investigated in the present study. Two antifreeze proteins (AFPI or AFPIII) were used at different concentrations of 0.1, 1, 10, and 100 μg/mL. We compared motility, curvilinear velocity, and plasma membrane integrity of fresh, cryopreserved sperm, and sperm cryopreserved in the presence of antifreeze proteins. Fresh sperm (control) had 85?±?4% motility and 160?±?2 μm/s curvilinear velocity, respectively. After cryopreservation, the motility of frozen-thawed sperm without addition of antifreeze proteins significantly decreased (44?±?9%), compared to the control. The highest motility of frozen-thawed sperm was obtained in cryopreserved sperm with addition of 1 μg/mL of AFPIII (58?±?14%). No significant differences were observed in curvilinear velocity between fresh sperm and cryopreserved sperm with/without addition of AFPI or AFPIII. The flow cytometry analysis revealed that fresh sperm contained 94.5?±?6% live cells, while the cryopreserved sperm only contained 26.6?±?14% live cells. Supplementation of antifreeze proteins has significantly improved the percentage of live cells in frozen-thawed sperm, except 0.1 μg/ml of AFPI group. No significant difference in percentage of live cells was detected in the sperm cryopreserved with 10 μg/mL of AFPI or AFPIII, compared to fresh sperm. Thus, addition of antifreeze proteins to cryopreservation medium could be considered to improve the post-thawed sperm quality of sterlet. 相似文献
2.
Kholodnyy Vitaliy Dzyuba Borys Gadêlha Hermes Cosson Jacky Boryshpolets Sergii 《Fish physiology and biochemistry》2021,47(3):653-669
Fish Physiology and Biochemistry - Fertilization of freshwater fish occurs in the environment which negatively affects a lifespan of gametes mostly due to the osmotic shock; therefore, male gametes... 相似文献
1