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Tanabe S Yamaguchi M Iijima M Nakajima S Sakata I Miyaki S Takemura T Furuoka H Kobayashi Y Matsui T Uzuka Y Sarashina T 《Veterinary journal (London, England : 1997)》2004,167(3):286-293
We describe here the detection by fluorescence of a new photosensitizer, PAD-S31, in tumours in dogs and cats and the effect of photodynamic therapy (PDT) by using PAD-S31 for skin tumours in two dogs and one cat. PAD-S31 is a hydrophilic photosensitizer that has two peaks at absorption wavelengths 406 and 665 nm in distilled water. In a preliminary experiment in mice transplanted with SCCVII and colon 26, PAD-S31 was retained in tumour tissues rather than in other organs. The tumours resected from dogs and cats after intravenous administration of PAD-S31 at a dose of 15 mg/kg emitted strong red fluorescence under light illumination of 402 nm wavelength. Animals given PAD-S31 showed no cutaneous photosensitivity under room light illumination. Irradiation at laser light 670 nm wavelength (fluence rate 150 mW/cm2 and total light dosage 150 J/cm2) on cutaneous mast cell tumours in dogs ( n=2 ) and a cutaneous basal cell tumour in a cat induced complete remission. These results suggest PAD-S31 could be a promising photosensitizer for use in a small animal veterinary practice. 相似文献
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Skeletal development and deformities in cultured larval and juvenile seven-band grouper, Epinephelus septemfasciatus (Thunberg) 总被引:1,自引:0,他引:1
Naoki Nagano Akiko Hozawa Wataru Fujiki Toshiyuki Yamada Kadoo Miyaki Yoshitaka Sakakura & Atsushi Hagiwara 《Aquaculture Research》2007,38(2):121-130
The seven‐band grouper, Epinephelus septemfasciatus (Thunberg), is currently recognized as a potential new species for aquaculture in Japan. This study describes normal and abnormal skeletal development of the jaw and vertebrae in cultured larvae and early juveniles of E. septemfasciatus. The ontogenetic stages at which skeletal deformities of jaw and vertebra developed were also described for this species. Osteological observations were made using a clearing and staining method for larvae and soft X‐ray photographs for juveniles. A high incidence of skeletal deformities was observed in the jaws and vertebral column during the larval and juvenile stages. Most of the jaw deformities were explained by an abnormal maxilla curvature. Jaw deformities were visually evident from flexion stage after ossification of the deformed elements. Deformities in the vertebral column (mostly lordosis) were observed from the post‐larval stage and became more evident as growth proceeded. The lordosis generally occurred on the positions of the 8–11th vertebra. These types of deformities are compared with those of other species, and possible causative factors of the skeletal deformities are discussed. 相似文献
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A charge-mosaic membrane was prepared from a pentablock copolymer of the BABCB type by selectively introducing anion and cation exchange groups into the microseparated phases. The three-layer lamellar structure of the starting pentablock copolymer film was not disturbed by the modifications. The membrane obtained was highly permeable only to sodium chloride in mixed aqueous solutions of sodium chloride and organic species of low molecular weight, such as sucrose. Marked pH-dependent permeabilities were also observed for amino acids. 相似文献
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The afferent veins to the liver of 325 adult fowls belonging to ten breeds were investigated by celluloid and neoprene latex casts, and venography. The author elucidates macroscopically the basic pattern of the hepatic portal vein. The right and left portal veins ramify the main branches to the liver, therefore, the intrahepatic distribution is divided into the two portal territories varying in relative size, but this change relates to the development of the medial branch from the right portal vein and the presence of the hepatic fissure. 相似文献
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Shinjiro Kanazawa Ngo Thi Tuong Chau Shintaro Miyaki 《Soil Science and Plant Nutrition》2005,51(5):671-674
From acidic tea soils of Kagoshima Prefecture in Japan, some soil properties were determined and 38 strains of acid tolerant microorganisms were isolated. Different Al3+ concentrations were applied to YG media to estimate Al resistance. Selected microbial strains could grow strongly in the liquid media in the presence of 100 mM Al3+ and survive even in 300 mM Al3+ at pH 3.0. Their base sequences of 28S rDNA-D1/D2 were determined and sequence data were searched using the Basic Local Alignment Search Tool (BLAST) system. The results of sequencing revealed that the isolates belong to two different species, Cryptococcus sp. and Candida palmioleophila. When cultivated with various Al3+ concentrations, the yeast growth was inhibited at a concentration of 200 mM. Pre-cultivation of these strains with 0–30 mM Al3+ did not promote the growth response caused by Al3+ . Inductively-Coupled Plasma-Mass Spectrometry (ICP-MS) was used to assess the elimination of Al. The amount of Al remaining in culture media was decreased considerably after cultivation. Due to a capacity for resistance to significant Al concentrations as well as high Al elimination, these acid tolerant and Al resistant yeasts may have potential applications in the bio- and phyto-remediation of Al and acid-contaminated soils. 相似文献
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Ramya Prashant Narendra Kadoo Charushila Desale Prajakta Kore Harcharan Singh Dhaliwal Parveen Chhuneja Vidya Gupta 《Journal of Cereal Science》2012
Wheat kernel size and shape influence its flour yield and market price. A hexaploid wheat population of 185 recombinant inbred lines was evaluated for five kernel morphometric traits namely, 1000-kernel weight, kernel length, width, length–width ratio and factor form density in two diverse agro-climatic regions in India in five to eight year–location combinations. Additive main effects and multiplicative interaction analysis revealed significant contributions from genotype (G) and genotype × environment (G × E) effects for these traits. Quantitative trait locus (QTL) analysis by composite interval mapping (CIM) was performed using a linkage map of 251 SSR markers and 59 QTLs distributed on 16 chromosomes were identified. The majority of the QTLs were located on the D genome (44.07%) and the homeologous chromosomes of Group 2 (38.98%). Stable QTLs detected in three or more year–location combinations were identified for four traits. Multi-trait CIM showed 10 chromosomal regions harboring putative pleiotropic loci. Complexity in the genetic effects was further revealed by QTL analysis based on mixed-linear model that indicated 19 QTLs with significant individual effects (main-effect QTLs) and 14 QTL × QTL interactions. Five of these nineteen main-effect QTLs and one of the fourteen QTL × QTL interactions showed environmental influence. 相似文献
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Shinjiro Kanazawa Ngo Thi Tuong Chau Shintaro Miyaki 《Soil Science and Plant Nutrition》2005,51(4):507-513
From acidic tea soils in Kagoshima Prefecture in Japan, 38 strains of acid-tolerant microorganisms were isolated and some soil properties were determined. Different Al concentrations were applied to YG media to estimate the Al resistance. Selected microbial strains were able to grow actively in liquid media in the presence of 100 mM Al and to survive even in 300 mM Al at pH 3.0. The base sequences of 28S rDNA-D1/D2 were determined and sequence data were searched using the Basic Local Alignment Search Tool (BLAST) system. The results of sequencing revealed that the isolates belonged to two different species, Cryptococcus sp. and Candida palmioleophila . Subsequently, Neighbor-Joining molecular phylogenetic trees were constructed. When the yeasts were cultured at various Al concentrations, the growth was inhibited at a concentration of 200 mM. Pre-culture of these strains with 0–30 mM Al did not promote the growth response induced by Al. Inductively coupled plasma-mass spectrometry (ICP-MS) was used to assess the elimination of Al from YG media. The amount of Al remaining in the culture media decreased considerably after culture. Due to a capacity for resistance to significant Al concentrations as well as high Al elimination, these acid-tolerant and Al-resistant yeasts may offer potential applications for the bio- and phyto-remediation of acid soils with a high level of Al. 相似文献
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We found that the corolla of petunia (Petunia hybrida Vilm.) could be conspicuously enlarged by the separate application of three cytokinins: forchlorfenuron (CPPU), N6-benzylaminopurine (BA), and zeatin. To obtain the same enlargement as that achieved by CPPU, approximately 30 and 900 times the concentration of BA and zeatin, respectively, were required. CPPU at 3.2 μmol/L increased the limb area of the corollas of 15 cultivars to between 1.3 and 2.4 times (1.8 times on average) the size of the control area. The increase was negatively correlated (R = 0.58) with the “genetic” limb area (i.e., that of the untreated plant). The enlargement of the corolla caused by cytokinin application was mainly attributed to an increase in cell number in most cultivars. This increase resulted from a high rate of cell proliferation and from prolongation of the cell proliferation phase during corolla development. This anatomical change caused by cytokinin application was similar to the anatomical difference among cultivars because genetic differences in limb area resulted mainly from differences in cell number. 相似文献